Molecular Cloning of Plastidial Large Subunit Ⅱ Gene and Its Constructions of Antisense and RNAi Expression Vectors

doi:10.7668/hbnxb.2009.04.001

Abstract

Abstract: Partial cDNA fragment (535 bp) of plastidial large subunit of AGPase (LSU II) from grains of common wheat variety Yujiao 2 was cloned (GenBank No. EF378944). The sequence analysis demonstrated that the cloned LSU II shared 88 % - 98 % homogeneity with the sequences of DQ409820,U66876,AK069296,DQ406819 genes. The antisense expression vector (pWM101LSU IIA) was constructed with pWM101 plasmids,and RNAi vector (pFGC5941LSU IISA) was also constructed with pFGC5941. The constructed vectors provided a good background for studying the function of LSU II.

Key words: Wheat ( Triticum aestivum), AGPase, LSU II, Plant expressing vectors

CLC Number:

Q785

LIU Chao, KANG Guo-zhang, GUO Tian-cai, YUE Cai-feng, SHEN Bing-quan. Molecular Cloning of Plastidial Large Subunit Ⅱ Gene and Its Constructions of Antisense and RNAi Expression Vectors[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 1-6. doi: 10.7668/hbnxb.2009.04.001.

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