Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2008, Vol. 23 ›› Issue (5): 147-150. doi: 10.7668/hbnxb.2008.05.032

Special Issue: Biotechnology；

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Cloning and Analysis ofIE-1Promoter from Nuclear Polyhedrosis Virus of Antherea pernyi

LENG Chun-ling1, LI Wen-li2   

  1. 1. Department of Biology, School of Agriculture, Eastern Liaoning University, Dandong 118003, China;
    2. College of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China
  • Received:2008-06-28 Published:2008-10-28

Abstract: A 2.7 kb fragment of IE promoter(IE-1)was got from ApNPV by PCR and subcloned into pMD18-T and sequenced.An expression vector ofIE-1/pGFP-N2 was constructed after 1.6 kb fragment of 3' flanking region of IE promoter was inserted into pGFP-N2 atEcoRI andBglII sites.GFP was expressed in Hela cells after transfected,which proved thatIE-1can active the expression of foreign gene.

Key words: IE promoter, ApNPV, pGFP-N2 expression vector

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Cite this article

LENG Chun-ling, LI Wen-li. Cloning and Analysis ofIE-1Promoter from Nuclear Polyhedrosis Virus of Antherea pernyi[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 147-150. doi: 10.7668/hbnxb.2008.05.032.

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