Genetic Purity Testing of Seed from Two-line Hybrid Rice( Oryza spp.) of Variety Peizaminkezhan

doi:10.7668/hbnxb.2008.03.016

Abstract

Abstract: Ultrathin-layer isoelectric focusing of seed proteins(UTLIEF)test were used to test the genetic purity of hybrid rice seeds.Results showed:Genetic purity determined by UTLIEF was the same as that of growout test and UTLIEF cost less money and fewer hours than grow-out test.

Key words: Ultra-thin layer isoelectric focusing, Genetic purity, Hybrid rice

CLC Number:

S511.04

YAN Min, ZHAO Yan-ming. Genetic Purity Testing of Seed from Two-line Hybrid Rice( Oryza spp.) of Variety Peizaminkezhan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 60-62. doi: 10.7668/hbnxb.2008.03.016.

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References

[1] Radola B J.Ultrathin-layer isoeleetric focusing in 50-100 μm polyacrylamide gels on silanized glass plates or polyester films[J].Electrophoresis,1980,1:43-56.

[2] Berg van den B M.A rapid and economical method for hybrid purity testing of tomato (Lycopersicon esculentum L.) F1hy-brids using ultrathin-layer isoelectric focusing of alcohol dehy-drogenase variants from seeds[J].Eleetrophoresis,1991 (12):64-69.

[3] Wang X F,Knoblauch R,Leist N.Varietal discrimination of tomato(Lycopersicon esculentum L.)by ultrathin-layer isoelec-tric focusing of seed protein[J].Seed Science and Technolo-gy,2000,28:201-213.

[4] Hahn H,Schoberlein W.Characterization and identification of Festulolium hybrids by electrophoresis of seed protein[J].Seed Science and Technology,1999,27(2):525-542.

[5] Lucchese C,Dinelli G,Miggiano A.Identification of pepper (Capsium spp.)cultivars by field and electrophoresis tests[J].Seed Science and Technology,1999 (27):37-47.

[6] 严敏,王晓峰.杂交玉米、水稻和辣椒种子品种真实性和纯度的室内快速鉴定[J].华南农业大学学报:自然科学版,2003,24(2):6-8.

[7] 刘敏轩,王赟文,韩建国等.饲用燕麦(Avenasativa.)品种的种子醇溶蛋白超薄层等电聚焦电泳快速鉴定[J].子,2006,25(9):5-8.

[8] 刘敏轩,韩建国,王赟文,等.超薄层等电聚焦电泳技术检验燕麦种子的真实性[J].草地学报,2007,15(1):7-12.

[9] Kang M,Jiang M X,Huang H W.Genctic diversity in frag-mented populations of Berchemiella wilsonii vat,pubipetiolata (Rhamnaceae)[J].Annals of Botany,2005,95:1145-1151.

[10] 王晓峰,黄惠玲,Knoblanch R,等.超薄等电聚焦电泳技术在水稻品种鉴定上的运用[J].种子,2000,19(4):6-9.

[11] 胡群宝,夏清华,陈森.用等电聚焦技术鉴定杂交稻华优桂99的种子纯度[J].植物生理学通讯,2004,40(5):597-598.

[12] 胡群宝,夏清华,陈森,等.利用等电聚焦技术对杂交稻丰优428种子纯度的快速鉴定[J].杂交水稻,2004,19(3):55-56.

[13] 胡群宝,夏清华,陈森,等.杂交稻博优998种子真实性与纯度的IEF鉴定[J].中国农学通报,2004(4):158-159.

[14] 胡群宝,夏清华,陈森,等.特优系列杂交稻种子纯度的鉴定[J].植物生理学通讯,2005(6):813-814.

[15] Zhao T,Yan M,Lu Y P,et al.Genetic purity testing of two-line hybrid rice seeds by uhrathin-layer isoelectric focusing of proteins[J].Seed Science and Technology,2005,33 (1):45-52.

[16] Yan M,Ye H,Wang X F.Invcstigation into the feasibility of assessing the hybridity of immature rice grains[J].Seed Sci-ence and Technology,2006,34(1):1-8.

[17] Dadlani M,Vashisht V,Singh D P,et al.A comparison of field grow-out and electrophnresis methods for testing genetic purity of cotton hybrid seed[J].Seed Research,1994,22 (2):160-162.

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