Abstract: Anthers of 40 barley varieties were innoculated on MS Medinm Supplemented with 2mg/L 2,4-D and 0.5mg/L KT to exame genotypical difference and select genotypes with better ability of producing calli. There were thirty one varieties showed calli. The highest callus inducing frequeney got to 49%. The green plantlet inducing frequency was as high as 1.4%.Different varieties had various responses on MS, B5 and C17, N6 Media. Our results indicated that B5 and N6 Media were more effective for barley pollen callus formation than MS and C17 Media. It was found that reducing NH3+ contents or increacing N03- contents could promote pollen callus production.Culture temperature could influence the development of microspores in vitro. For some genotypes, the callus production could be promoted when innoculated anthers were treated with high temperature (30℃) for five days, but for others, the callus formation frequency could be enhanced when treated with low temperature (15℃).2, 4 -D was an essential factor in inducing pollen callus formation in barley as showed in our experiment.

Key words: Barley, Anther culture, Genotypes, Culture condition