Identification of BAC Clones Related with Verticillium Wilt Resistance Geneand Construction of Sub-clone Library

doi:10.3321/j.issn:1000-7091.2006.z1.035

Abstract

Abstract: In the study, the fragment PR8 related with Verticillium wilt resistance was used as a probe to screen Bacterial Artificial Chromosome (BAC) library of a Sea Island cotton, Pima90-53, with good quality and high Verticillium wilt resistance. Four positive BAC clones, 127K13, 128D14, 169J3 and 178C5, were detected from 30336 BAC clones. The BAC clone, 169J3, was digested with Sau3AI. After digestion, 2-4 kb fragments were collected and ligated with pUC118BamHI2BAP to construct a sub-clone library. The library contained 4224 clones, and inserts DNA ranged from 1 kb to 3 kb, averaged 2 kb. The sub-clone library could be used for obtaining the complete sequence of Verticillium wilt resistance gene.

Key words: Cotton, Verticillium wilt, BAC, Sub-clone library, Gene cloning

CLC Number:

S562.01

WANG Wen-sheng, WANG Xing-fen, MA Zhi-ying, ZHANG Gui-yin. Identification of BAC Clones Related with Verticillium Wilt Resistance Geneand Construction of Sub-clone Library[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 147-150. doi: 10.3321/j.issn:1000-7091.2006.z1.035.

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References

[1] 马峙英, 王省芬, 张桂寅, 等. 不同来源海岛棉品种黄萎病抗性遗传研究[J]. 作物学报, 2000, (26): 315-321.
[2] 房卫平, 祝水金, 季道藩. 陆地棉和海岛棉的黄萎病抗性遗传研究[J]. 棉花学报, 2003, 15(1): 3-7.
[3] 王晓萍, 温玉琴, 郭东林. 植物抗病基因研究进展[J]. 黑龙江农业科学, 2003, (4): 42-45.
[4] Giraudat J, Hauge B, Valon C, et al. Isolation of the Arabidopsis ABI3 gene by positional cloning[J]. Plant Cell, 1992, 4: 1251-1261.
[5] Mozo T, Dewar K, Dunn P, et al. A complete BAC-based physical map of the Arabidopsis thaliana genome[J]. Nat Genet., 1999, 22: 271-275.
[6] Wu C, Sun S, Nimmakayala P, et al. A BAC-and BIBAC-based physical map of the soybean genome[J]. Genome Research, 2004, 14 (2): 319-326.
[7] Wu C, Nimmakayala P, Santos F A, et al. Construction and characterization of a soybean bacterial artificial chromosome library and use of multiple complementary libraries for genome physical mapping[J]. Theor Appl Genet., 2004, 108: 1432-2242.
[8] Osoegawa K, Mammoser A G, WU Chen-yan, et al. A bacterial artificial chromosome library for sequencing the complete human genome[J]. Genome Research, 2001, 11 (3): 483-496.
[9] Lichtenzveig J, Scheuring C, Dodge J, et al. Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea, Cicer arietinum L[J]. Theor Appl Genet, 2005, 110 (3): 492-510.
[10] 王省芬, 郑拥民, 张桂寅, 等. 棉花细菌人工染色体文库构建的方法优化[J]. 棉花学报, 2004, 16 (3): 170-174.
[11] Tao Q, Zhao H, Qiu L, et al. Construction of a full bacterial artificial chromosome (BAC) library of Oryza sativa genome[J]. Cell Res, 1994, 4: 127-133.
[12] Zhang H B, Choi S, Woo S S, et al. Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population[J]. Mol Breeding, 1996, 2: 11-24.
[13] 王世全, 李平, 翟文学, 等. 长片段水稻细菌人工染色体DNA的亚克隆文库的构建[J]. 四川农业大学学报, 2000, 18 (3): 197-200.

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