Abstract:
Tw o-stage culture method w as used to study the ef fect of types and concentration of hormone, effect of types of culture medium, and nut rit ion on shikonin format ion. The result s show that both cytokinin and aux in are needed in shikonin formation. 6-BA 110 mg#L-1 and IAA 011 mg#L-1 is suitable in shikonin format ion medium. B5 medium is a better medium for cell growth. B5 w ith 250 mg#L-1 of CaCl2, 500 mg#L-1 of Mg SO4 are bet ter for both growth and shikonin format ion. M9 w ith three t imes of concentration of CuSO4 are bet ter choice for shikonin format ion. Addition of VB2 and more FeSO4 has no posit ive ef fect on shikonin format ion.
Key words:
L i thospermum erythroriz on,
Shikonin,
Cell culture
CLC Number:
YAN Haiyan, CAO Riqiang. Factors on Shikonin Formation in Cell Culture of Lithospermum erythrorizon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(2): 116-120. doi: 10.3321/j.issn:1000-7091.2002.02.025.