Insect Resistant Stock Selected by Introducing Dogbane DNA into Cotton

doi:10.3321/j.issn:1000-7091.1999.04.007

Abstract

Abstract: After self-pollination twenty four hours,the exogenous bagbane(A.Venetum L.)DNA was introduced into ovaries of variety lumian 6(G.hirsutum)by injection method.In high selection stress,the second generation was screened for insect resistant by using natural insect.The stock 115 with resistance to bollworm and aphid was selected.Laboratory bioassay and food utilization of bollworm were used to test 115 and lumian 6.The results showed that leaf square and small boll of 115 highly inhibited food utilization of cotton bollworm,and significantly reduced larval surial,growth and development,whereas the effect was small in lumian 6.Contents of gossypol and tani in leaf of 115 were 90.9% and 136.7% as much as that of Lumian 6.

Key words: Cotton, Dogbane, DNA, Gene transfer, Insect resistant

CLC Number:

S562.034

Shen Fafu, Yu Yuanjie, Zhang Xuekun, Liu Fengzhen, Yin Chengyi. Insect Resistant Stock Selected by Introducing Dogbane DNA into Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(4): 34-38. doi: 10.3321/j.issn:1000-7091.1999.04.007.

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