华北农学报 ›› 2020, Vol. 35 ›› Issue (2): 170-177. doi: 10.7668/hbnxb.20190344

所属专题: 葡萄

• 资源环境·植物保护 • 上一篇    下一篇

荒漠区滴灌施氮量对葡萄叶绿素荧光特性及碳代谢的影响

韩丽娜1, 马宗桓1, 王颖1, 胡紫璟1, 史星雲2, 毛娟1, 陈佰鸿1   

  1. 1. 甘肃农业大学 园艺学院, 甘肃 兰州 730070;
    2. 武威市林业科学研究院, 甘肃 武威 733000
  • 收稿日期:2019-12-16 出版日期:2020-04-28
  • 通讯作者: 陈佰鸿(1969-),男,甘肃武威人,教授,博士,博士生导师,主要从事果树生物技术及栽培生理研究。
  • 作者简介:韩丽娜(1994-),女,甘肃白银人,在读硕士,主要从事果树逆境生理与生长调控研究。
  • 基金资助:
    甘肃省葡萄酒发展专项(039-041006);国家自然科学基金项目(31460499);甘肃省科技重大专项(18ZD2NA006);甘肃省林业科技计划(2015kj023)

Effects of Nitrogen Application Rate in Drip Irrigation on Chlorophyll Fluorescence Characteristics and Carbon Metabolism of Grape in Desert Area

HAN Lina1, MA Zonghuan1, WANG Ying1, HU Zijing1, SHI Xingyun2, MAO Juan1, CHEN Baihong1   

  1. 1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China;
    2. Wuwei Academy of Forestry Science, Wuwei 733000, China
  • Received:2019-12-16 Published:2020-04-28

摘要: 为了研究蛇龙珠葡萄各生育期施氮量对叶片光合特性和同化产物积累的影响,在滴灌条件下,设定4个尿素施用量:150(N1),300(N2),450(N3),600 kg/hm2(N4),对照(CK)设置为0 kg/hm2,分析各生育期(花前5 d DBF5、花后20 d DAF20和花后50 d DAF50)不同氮素营养对葡萄叶片的光合荧光参数及碳代谢相关指标的影响。结果表明:适量增施氮肥提高了叶片叶绿素含量和RuBp羧化酶活性,增强了葡萄树的光合作用,而叶片中PSⅡ有效光化学效率(Fv'/Fm')、光化学淬灭系数(qP)、PSⅡ实际光化学效率(ΦPSⅡ)的提高及非光化学淬灭系数(NPQ)的降低使叶片叶绿素荧光增强,且N2下的Fv'/Fm'、ΦPSⅡ、qP在DAF20和DAF50时比同期CK分别显著升高了16.10%,17.80%;21.49%,21.13%及11.77%,19.73%;适量增施氮肥提高了蔗糖代谢相关酶的活性和可溶性糖含量,减少了淀粉含量,且在整个生育期,SPS、SS以及蔗糖在N3下有最高值,葡萄糖和果糖在N2下有最高值,而在DBF5、DAF20和DAF50时NI和AI分别在N2、N2和N1处理下有最高值。同CK相比,N2处理显著增加了可溶性糖、单宁、花青素的含量和可滴定酸含量,提高了坐果率和糖酸比,产量达14.50 t/hm2。综上,在300~450 kg/hm2的尿素施用范围内明显增强了叶片光合作用,促进可溶性糖的合成,从而为葡萄果实的生长发育供应足够的营养。

关键词: 葡萄, 氮素, 叶绿素荧光, 碳代谢, 产量品质

Abstract: In order to study the effects of nitrogen application rate on the photosynthetic characteristics and assimilation product accumulation of Cabernet gernischet grapes at different growth stages, four urea application rates under drip irrigation conditions were set:150(N1), 300(N2), 450 (N3), 600 kg/ha(N4), 0 kg/ha was set to control(CK), the effects of different nitrogen nutrition on photosynthetic fluorescence parameters and carbon metabolism related indexes of grape leaves at growth period (5 days before flowering,20 days after flowering,50 days after flowering) were analyzed. The results showed that appropriate nitrogen application improved the chlorophyll content and RuBp carboxylase activity of leaves, enhanced the photosynthesis of vines, and increased the chlorophyll fluorescence of leaves which represented as the increase of effective photochemical efficiency (Fv'/Fm'), photochemical quenching coefficient (qP) and actual photochemical efficiency of PSⅡ(ΦPSⅡ) and the decrease of non-photochemical quenching coefficient (NPQ) in leaves. Compared with CK, Fv'/Fm', ΦPSⅡ, and qP under N2 treatment in DAF20 and DAF50 were significantly increased by 16.10%, 17.80%, and 21.49%, 21.13%, and 11.77%, 19.73%, respectively.Appropriate nitrogen application increased the activity of sucrose metabolism-related enzymes and soluble sugar content, but decreased starch content.And throughout the growth period, SPS, SS, and sucrose had the highest values under N3, glucose and fructose had the highest values under N2, while NI and AI had the highest values under N2, N2 and N1 respectively under DBF5, DAF20 and DAF50. Compared with CK, N2 treatment increased the content of soluble sugar, tannin, anthocyanin and the titratable acid content, promoted the fruit setting rate and the ratio of sugar to acid with yield of 14.50 t/ha.In conclusion, the application range of urea from 300 to 450 kg/ha enhanced the photosynthesis of leaves, promoted the synthesis of soluble sugars, thus providing sufficient nutrition for the growth and development of grape fruits.

Key words: Grape, Nitrogen, Chlorophyll fluorescence, Carbon metabolism, Yield quality

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引用本文

韩丽娜, 马宗桓, 王颖, 胡紫璟, 史星雲, 毛娟, 陈佰鸿. 荒漠区滴灌施氮量对葡萄叶绿素荧光特性及碳代谢的影响[J]. 华北农学报, 2020, 35(2): 170-177. doi: 10.7668/hbnxb.20190344.

HAN Lina, MA Zonghuan, WANG Ying, HU Zijing, SHI Xingyun, MAO Juan, CHEN Baihong. Effects of Nitrogen Application Rate in Drip Irrigation on Chlorophyll Fluorescence Characteristics and Carbon Metabolism of Grape in Desert Area[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 170-177. doi: 10.7668/hbnxb.20190344.

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