Cloning and Expression of ZmPP2C6-01 Gene in Maize
YUAN Zhen1, ZHANG Pengyu1,2, WANG Guorui1, CAO Liru1,2,3, KU Lixia1,2, WEI Li1
1. Agricultural College of Henan Agricultural University, National Research Center of Wheat Engineering Technology, Zhengzhou 450046, China; 2. Henan Collaborative Innovation Center for Food Crops, Zhengzhou 450046, China; 3. Henan Academy of Agricultural Sciences, Zhengzhou 450046, China
Abstract：In order to study the response of maize serine/threonine protein phosphatase PP2C gene to abiotic stress and its physiological function, the transcriptome of maize inbred line Yu 882 was analyzed under 20% PEG6000 stress. The PP2C gene strongly expressed under drought stress was screened and cloned, while the bioinformatics analysis and expression pattern analysis were carried out. A PP2C protein gene that was up-regulated significantly by drought stress was found in the 20% PEG6000 stress-treated transcriptome of maize, and was named as ZmPP2C6-01. The open reading frame of ZmPP2C6-01 gene was 1 242 bp, encoding 413 amino acids. The encoded protein had a molecular mass of 43.8 ku and an isoelectric point of 6.6. It was a hydrophilic protein. The ZmPP2C6-01 protein was closely related to the PP2C protein of sorghum, and also had the close relationship with the PP2C protein of winter wheat and Aegilops tauschii. The ZmPP2C6-01 protein was localized in the nucleus. Real-time quantitative PCR analysis showed that ZmPP2C6-01 gene had the highest expression in maize roots, followed by leaves and stems. Under the condition of PEG stress, the expression level of ZmPP2C6-01 gene in roots was higher than that of the control in most of the time, and it was up-regulated in leaves. However, under the stresses of ABA, NaCl and high temperature, the expression level of ZmPP2C6-01 gene in roots was lower than that of the control, and the overall expression showed a downward trend, while the expression level in leaves was higher than that of the control, showing an up-regulated expression pattern. In summary, the ZmPP2C6-01 gene responded to drought and other stresses, but the expression patterns were inconsistent.
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2019, Vol. 34 
