摘要： 为了研究玉米蛋白磷酸酶2C（PP2C）基因对非生物胁迫的响应及生理功能，以耐旱玉米自交系豫882为试验材料，对玉米20% PEG6000胁迫处理转录组进行分析，筛选受干旱诱导强烈表达的PP2C基因，然后对其进行克隆、生物信息学分析及表达模式分析。结果表明，在玉米20% PEG6000胁迫处理转录组中筛选到一个干旱胁迫下显著上调表达的PP2C蛋白基因，命名为ZmPP2C6-01。ZmPP2C6-01基因的开放阅读框为1 242 bp，编码413个氨基酸，编码蛋白质的分子质量为43.8 ku，等电点为6.6，是一种亲水性蛋白。ZmPP2C6-01蛋白与高粱的PP2C蛋白亲缘关系最近，而与冬小麦、粗山羊草等的PP2C蛋白亲缘关系相对较远。ZmPP2C6-01蛋白定位于细胞核中。利用实时荧光定量PCR分析发现，ZmPP2C6-01基因在玉米根中的表达量最高，其次是叶，茎中最低；PEG胁迫下，ZmPP2C6-01基因在根中的表达量大部分时间点均高于对照，同时在叶中呈上调表达模式；在ABA、NaCl、高温胁迫下，ZmPP2C6-01基因在根中大部分时间点的表达量均低于对照，整体呈下降趋势，而在叶中的表达量均高于对照，呈上调表达模式。综上表明，ZmPP2C6-01基因响应干旱等逆境胁迫，但表达模式不一致。

关键词: 玉米, ZmPP2C6-01, 克隆, 亚细胞定位, 非生物胁迫, 表达分析

Abstract: In order to study the response of maize serine/threonine protein phosphatase PP2C gene to abiotic stress and its physiological function, the transcriptome of maize inbred line Yu 882 was analyzed under 20% PEG6000 stress. The PP2C gene strongly expressed under drought stress was screened and cloned, while the bioinformatics analysis and expression pattern analysis were carried out. A PP2C protein gene that was up-regulated significantly by drought stress was found in the 20% PEG6000 stress-treated transcriptome of maize, and was named as ZmPP2C6-01. The open reading frame of ZmPP2C6-01 gene was 1 242 bp, encoding 413 amino acids. The encoded protein had a molecular mass of 43.8 ku and an isoelectric point of 6.6. It was a hydrophilic protein. The ZmPP2C6-01 protein was closely related to the PP2C protein of sorghum, and also had the close relationship with the PP2C protein of winter wheat and Aegilops tauschii. The ZmPP2C6-01 protein was localized in the nucleus. Real-time quantitative PCR analysis showed that ZmPP2C6-01 gene had the highest expression in maize roots, followed by leaves and stems. Under the condition of PEG stress, the expression level of ZmPP2C6-01 gene in roots was higher than that of the control in most of the time, and it was up-regulated in leaves. However, under the stresses of ABA, NaCl and high temperature, the expression level of ZmPP2C6-01 gene in roots was lower than that of the control, and the overall expression showed a downward trend, while the expression level in leaves was higher than that of the control, showing an up-regulated expression pattern. In summary, the ZmPP2C6-01 gene responded to drought and other stresses, but the expression patterns were inconsistent.

Key words: Zea mays, ZmPP2C6-01, Subcellular localization, Abiotic stress, Expression analysis

中图分类号: 

• S513.03

• Q78