摘要： 用基因枪将人工构建的植物雄性不育基因TA29-Barnase及其标记基因bar导入芝麻豫芝4号的离体子叶，在MS+5mg·L^-16-BA+1mg·L^-1IAA+1mg·L^-1ABA+5mg·L^-1AgNO₃的培养基上交培养12天，转入光、暗光替培养，诱导出不定芽。经添加2mg·L^-1除草剂（BASTA）的上述培养基筛选，获得了11个BASTA抗性株系，对其中3个株系进行Southern杂交鉴定分析，证实其中2个为转基因株系，转化率为1.46%.

关键词: 芝麻, 基因枪, 离体子叶, Barnase基因, bar基因, 转基因芝麻植株

Abstract: The engineered male sterile gene barnase together with the mark gene bar were transformed to the isolated cotyledon of a sesane variety Yuzhi 4 by microprojectile bomebardment.The transformed cotyledon was cultured under darkness for 12 days then converted into dark 1ight alternative conditions on the culture media MS+5mg.L ^-1 6-BA+1mg•L^-1 IAA+1mg•L^-1 ABA+5mg•L - AgNO₃ Resistant calli were selected on herbicide Basta 2mg.L^-1 11 anti Basta plants were obtained.Southern blot of 3 well developed green plants confirmed stable integration of both barnase and bar genes into nuclear DNA.The transfomation efficiency reached 1.46%.

Key words: Sesame, Microprojectile bombardment, Cotyledon, Barnase gene, Bar gene, Transgenic sesame plant

中图分类号: 

• S565.3