棉花bHLH转录因子功能研究进展与展望

doi:10.7668/hbnxb.20195187

摘要/Abstract

摘要：

bHLH(Basic helix-loop-helix)转录因子家族是植物中广泛存在的超基因家族,能结合靶基因启动子上的顺式作用元件,从而广泛参与植物生长发育、次生代谢调控、信号转导和胁迫应答等各种生理生化过程。棉花作为世界上最重要的经济作物之一,枯/黄萎病、低温、高温、盐碱、干旱和重金属等胁迫严重影响棉花产量和纤维品质。同时,随着植棉效益的不断下降,棉籽和次生代谢物等副产品的高值化利用对提高棉花综合利用价值更具有关键意义。本研究对bHLH转录因子在棉花纤维、花药、色素腺体等组织器官发育及体细胞胚胎发生、株型调控、生物/非生物胁迫应答等生命活动中功能的研究进展进行了总结,并对深入解析bHLH转录因子生物学功能,提高棉籽油、棉籽蛋白、棉酚、花青素、棉籽维生素、花蜜等棉花副产品利用价值的应用前景进行了展望,这将为深入阐明bHLH转录因子在棉花生长发育等过程中的功能以及提升棉副产品的综合利用价值提供参考。

关键词: 棉花, 转录因子, bHLH, 研究进展

Abstract:

bHLH(Basic helix-loop-helix)gene family is one of the largest plant transcription factor families,binds to cis-acting elements on target gene promoters and plays crucial roles in various physiological processes including growth development,secondary metabolism regulation,signal transduction,and stress response.As one of the world's most important cash crops,cotton is severely affected by stresses such as Verticillium and Fusarium wilt,low temperature,high temperature,salinity,drought,and heavy metal exposure,which significantly impact yield and fiber quality.Meanwhile,with the continuous decline in cotton cultivation benefits,the high-value utilization of by-products such as cottonseeds and cotton secondary metabolites plays a crucial role in improving the comprehensive utilization value of cotton.In this paper,the functions of cotton bHLH transcription factors in cotton fiber development,biotic stress response,abiotic stress response,plant architecture,anther,glandular development,and somatic embryogenesis were summarized.Furthermore,it discusses the application prospects of in-depth analysis of the biological functions of bHLH transcription factors in enhancing the utilization value of cotton by-products,such as cottonseed oil,cottonseed protein,gossypol,anthocyanins,cottonseed vitamins,and nectar,in order to provide theoretical reference for further clarifying the functions of bHLH transcription factors in cotton growth and development,as well as improving the comprehensive utilization value of cotton by-products.

Key words: Cotton, Transcription factors, bHLH, Research progress

中图分类号:

S562
S961.6

甄军波, 刘琳琳, 刘迪, 迟吉娜. 棉花bHLH转录因子功能研究进展与展望[J]. 华北农学报, 2025, 40(S1): 54-60. doi: 10.7668/hbnxb.20195187.

ZHEN Junbo, LIU Linlin, LIU Di, CHI Jina. Research Progress and Prospects on the Function of bHLH Transcription Factor Family in Cotton[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 54-60. doi: 10.7668/hbnxb.20195187.

http://www.hbnxb.net/CN/Y2025/V40/IS1/54

参考文献 64

[1]	Lu R, Zhang J, Wu Y W, Wang Y, Zhang J, Zheng Y, Li Y, Li X B. bHLH transcription factors LP1 and LP2 regulate longitudinal cell elongation[J]. Plant Physiology, 2021, 187(4):2577-2591.doi:10.1093/plphys/kiab387. pmid: 34618066
[2]	Shen T J, Wen X P, Wen Z, Qiu Z L, Hou Q D, Li Z C, Mei L N, Yu H H, Qiao G. Genome-wide identification and expression analysis of bHLH transcription factor family in response to cold stress in sweet cherry(Prunus avium L.)[J]. Scientia Horticulturae, 2021, 279:109905.doi:10.1016/j.scienta.2021.109905. URL
[3]	Lu R, Li Y, Zhang J, Wang Y, Zhang J, Li Y, Zheng Y, Li X B. The bHLH/HLH transcription factors GhFP2 and GhACE1 antagonistically regulate fiber elongation in cotton[J]. Plant Physiology, 2022, 189(2):628-643.doi:10.1093/plphys/kiac088. pmid: 35226094
[4]	Wu H H, Fan L Q, Guo M Z, Liu L, Liu L S, Hou L Y, Zheng L, Qanmber G, Lu L L, Zhang J, Li F G, Yang Z R. GhPRE1A promotes cotton fibre elongation by activating the DNA-binding bHLH factor GhPAS1[J]. Plant Biotechnology Journal, 2023, 21(5):896-898.doi:10.1111/pbi.14005. URL
[5]	Ohashi-Ito K, Fukuda H. Functional mechanism of bHLH complexes during early vascular development[J]. Current Opinion in Plant Biology, 2016, 33:42-47.doi:10.1016/j.pbi.2016.06.003. pmid: 27314622
[6]	Guo X J, Wang J R. Global identification,structural analysis and expression characterization of bHLH transcription factors in wheat[J]. BMC Plant Biology, 2017, 17(1):90.doi:10.1186/s12870-017-1038-y. URL
[7]	Sun X, Wang Y, Sui N. Transcriptional regulation of bHLH during plant response to stress[J]. Biochemical and Biophysical Research Communications, 2018, 503(2):397-401.doi:10.1016/j.bbrc.2018.07.123. pmid: 30057319
[8]	Wang Z, Yang Z R, Li F G. Updates on molecular mechanisms in the development of branched trichome in Arabidopsis and nonbranched in cotton[J]. Plant Biotechnology Journal, 2019, 17(9):1706-1722.doi:10.1111/pbi.13167. pmid: 31111642
[9]	Carey-Fung O, O'Brien M, Beasley J T, Johnson A A T. A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network[J]. International Journal of Molecular Sciences, 2022, 23(3):1635.doi:10.3390/ijms23031635. URL
[10]	Das A K, Hao L. Functional characterization of ZmbHLH121,a bHLH transcription factor,focusing on Zea mays kernel development[J]. Gene Reports, 2022, 28:101645.doi:10.1016/j.genrep.2022.101645. URL
[11]	Zhang T T, Lyu W, Zhang H S, Ma L, Li P H, Ge L, Li G. Genome-wide analysis of the basic Helix-Loop-Helix(bHLH)transcription factor family in maize[J]. BMC Plant Biology, 2018, 18(1):235.doi:10.1186/s12870-018-1441-z.
[12]	Gong W F, Du X M, Jia Y H, Pan Z E. Color cotton and its utilization in China[M]//Cotton Fiber:Physics,Chemistry and Biology. Cham: Springer International Publishing,2018:117-132.doi:10.1007/978-030-00871-0_6.
[13]	Liu B L, Guan X Y, Liang W H, Chen J D, Fang L, Hu Y, Guo W Z, Rong J K, Xu G H, Zhang T Z. Divergence and evolution of cotton bHLH proteins from diploid to allotetraploid[J]. BMC Genomics, 2018, 19(1):162.doi:10.1186/s12864-018-4543-y. pmid: 29471803
[14]	Zhang B P, Chopra D, Schrader A, Hülskamp M. Evolutionary comparison of competitive protein-complex formation of MYB,bHLH,and WDR proteins in plants[J]. Journal of Experimental Botany, 2019, 70(12):3197-3209.doi:10.1093/jxb/erz155. URL
[15]	Yan Q, Liu H S, Yao D, Li X, Chen H, Dou Y, Wang Y, Pei Y, Xiao Y H. The basic/helix-loop-helix protein family in Gossypium:reference genes and their evolution during tetraploidization[J]. PLoS One, 2015, 10(5):e0126558.doi:10.1371/journal.pone.0126558.
[16]	Hu H Y, He X, Tu L L, Zhu L F, Zhu S T, Ge Z H, Zhang X L. GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like[J]. The Plant Journal, 2016, 88(6):921-935.doi:10.1111/tpj.13273. pmid: 27419658
[17]	Xia X C, Hu Q Q, Li W, Chen Y, Han L H, Tao M, Wu W Y, Li X B, Huang G Q. Cotton(Gossypium hirsutum)JAZ3 and SLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation[J]. Plant Cell,Tissue and Organ Culture(PCTOC), 2018, 133(2):249-262.doi:10.1007/s11240-018-1378-9.
[18]	Xiao G H, He P, Zhao P, Liu H, Zhang L, Pang C Y, Yu J N. Genome-wide identification of the GhARF gene family reveals that GhARF2 and GhARF18 are involved in cotton fibre cell initiation[J]. Journal of Experimental Botany, 2018, 69(18):4323-4337.doi:10.1093/jxb/ery219. URL
[19]	Lu R, Zhang J, Liu D, Wei Y L, Wang Y, Li X B. Characterization of bHLH/HLH genes that are involved in brassinosteroid(BR)signaling in fiber development of cotton(Gossypium hirsutum)[J]. BMC Plant Biology, 2018, 18(1):304.doi:10.1186/s12870-018-1523-y.
[20]	Liu Z H, Chen Y, Wang N N, Chen Y H, Wei N, Lu R, Li Y, Li X B. A basic helix loop helix protein(GhFP1)promotes fibre elongation of cotton(Gossypium hirsutum)by modulating brassinosteroid biosynthesis and signalling[J]. New Phytologist, 2020, 225(6):2439-2452.doi:10.1111/nph.16301. URL
[21]	Li M Y, Hao P B, Zhang J J, Yang X, Wu A M, Zhang M, Wei H L, Fu X K, Wang H T, Yu S X. A comprehensive identification and function analysis of the ATBS1 Interacting Factors(AIFs)gene family of Gossypium species in fiber development and under multiple stresses[J]. Industrial Crops and Products, 2021, 171:113853.doi:10.1016/j.indcrop.2021.113853. URL
[22]	MacMillan C P, Birke H, Chuah A, Brill E, Tsuji Y, Ralph J, Dennis E S, Llewellyn D, Pettolino F A. Tissue and cell-specific transcriptomes in cotton reveal the subtleties of gene regulation underlying the diversity of plant secondary cell walls[J]. BMC Genomics, 2017, 18(1):539.doi:10.1186/s12864-017-3902-4. pmid: 28720072
[23]	Gao Z Y, Sun W J, Wang J, Zhao C Y, Zuo K J. GhbHLH18 negatively regulates fiber strength and length by enhancing lignin biosynthesis in cotton fibers[J]. Plant Science, 2019, 286:7-16.doi:10.1016/j.plantsci.2019.05.020. pmid: 31300144
[24]	Sun H R, Hao P B, Gu L J, Cheng S S, Wang H T, Wu A M, Ma L, Wei H L, Yu S X. Pectate lyase-like Gene GhPEL76 regulates organ elongation in Arabidopsis and fiber elongation in cotton[J]. Plant Science, 2020, 293:110395.doi:10.1016/j.plantsci.2019.110395. URL
[25]	Liu W Y, Lyu Y J, Li X Y, Feng Z Q, Wang L C. Comparative transcriptome analysis uncovers cell wall reorganization and repressed cell division during cotton fiber initiation[J]. BMC Developmental Biology, 2021, 21(1):15.doi:10.1186/s12861-021-00247-3. pmid: 34715791
[26]	Zou X Y, Liu A Y, Zhang Z, et al. Co-expression network analysis and hub gene selection for high-quality fiber in upland cotton(Gossypium hirsutum)using RNA sequencing analysis[J]. Genes, 2019, 10(2):119.doi:10.3390/genes10020119. URL
[27]	Shangguan X X, Yang C Q, Zhang X F, Wang L J. Functional characterization of a basic helix-loop-helix(bHLH)transcription factor GhDEL65 from cotton(Gossypium hirsutum)[J]. Physiologia Plantarum, 2016, 158(2):200-212.doi:10.1111/ppl.12450. URL
[28]	Wang G, Zhao G H, Jia Y H, Du X M. Identification and characterization of cotton genes involved in fuzz-fiber development[J]. Journal of Integrative Plant Biology, 2013, 55(7):619-630.doi:10.1111/jipb.12072.
[29]	严倩. 棉花棕色纤维基因Lc1的图位克隆及原花色素合成和纤维棕色呈色的调控[D]. 重庆: 西南大学,2016:1-9.
	Yan Q. Map-based cloning of dark brown fiber gene Lc1,and the regulation of proanthocyanidin biosynthesis and brown fiber coloration[D]. Chongqing: Southwest University,2016:1-9.
[30]	Liu Y J, Hou H, Jiang X L, Wang P Q, Dai X L, Chen W, Gao L P, Xia T. A WD40 repeat protein from Camellia sinensis regulates anthocyanin and proanthocyanidin accumulation through the formation of MYB bHLH WD40 ternary complexes[J]. International Journal of Molecular Sciences, 2018, 19(6):1686.doi:10.3390/ijms19061686. URL
[31]	Shangguan X, Yang Q, Wu X, Cao J. Function analysis of a cotton R2R3 MYB transcription factor GhMYB3 in regulating plant trichome development[J]. Plant Biology, 2021, 23(6):1118-1127.doi:10.1111/plb.13299. pmid: 34396658
[32]	Yan Q, Wang Y, Li Q, Zhang Z S, Ding H, Zhang Y, Liu H S, Luo M, Liu D X, Song W, Liu H F, Yao D, Ouyang X F, Li Y H, Li X, Pei Y, Xiao Y H. Up-regulation of GhTT2-3A in cotton fibres during secondary wall thickening results in brown fibres with improved quality[J]. Plant Biotechnology Journal, 2018, 16(10):1735-1747.doi:10.1111/pbi.12910. URL
[33]	University S P S, Mikhailova A, Strygina K, Khlestkina E. The genes determining synthesis of pigments in cotton[J]. Biological Communications, 2019, 64(2):133-145.doi:10.21638/spbu03.2019.205. URL
[34]	Ke L P, Yu D L, Zheng H L, Xu Y H, Wu Y Q, Jiao J Y, Wang X L, Mei J, Cai F F, Zhao Y Y, Sun J, Zhang X L, Sun Y Q. Function deficiency of GhOMT1 causes anthocyanidins over-accumulation and diversifies fibre colours in cotton(Gossypium hirsutum)[J]. Plant Biotechnology Journal, 2022, 20(8):1546-1560.doi:10.1111/pbi.13832. URL
[35]	刘厚生. 棉花bHLH蛋白基因的克隆、表达及生物信息学分析[D]. 重庆: 西南大学,2014:27-39.
	Liu H S. Cloning,expression and bioinformatic analysis of bHLH protein genes in cotton[D]. Chongqing: Southwest University,2014:27-39.
[36]	Wang Z Z, Rehman A, Jia Y H, Dai P H, He S P, Wang X Y, Li H G, Wang L R, Qayyum A, Peng Z, Du X M. Transcriptome and proteome profiling revealed the key genes and pathways involved in the fiber quality formation in brown cotton[J]. Gene, 2023, 868:147374.doi:10.1016/j.gene.2023.147374. URL
[37]	Zhao B, Cao J F, Hu G J, Chen Z W, Wang L Y, Shangguan X X, Wang L J, Mao Y B, Zhang T Z, Wendel J F, Chen X Y. Core cis-element variation confers subgenome-biased expression of a transcription factor that functions in cotton fiber elongation[J]. New Phytologist, 2018, 218(3):1061-1075.doi:10.1111/nph.15063. pmid: 29465754
[38]	Wu H H, Ren Z Y, Zheng L, Guo M Z, Yang J Y, Hou L Y, Qanmber G, Li F G, Yang Z R. The bHLH transcription factor GhPAS1 mediates BR signaling to regulate plant development and architecture in cotton[J]. The Crop Journal, 2021, 9(5):1049-1059.doi:10.1016/j.cj.2020.10.014. URL
[39]	Meng C M, Zhang T Z, Guo W Z. Molecular cloning and characterization of a novel Gossypium hirsutum L.bHLH gene in response to ABA and drought stresses[J]. Plant Molecular Biology Reporter, 2009, 27(3):381-387.doi:10.1007/s11105-009-0112-5. URL
[40]	孙娜, 王劲, 左开井. 陆地棉GhbHLH4基因的克隆与功能分析[J]. 中国农业科技导报, 2014, 16(3):29-35.doi:10.13304/j.nykjdb.2013.593.
	Sun N, Wang J, Zuo K J. Cloning and functional analysis of GhbHLH4 gene from upland cotton[J]. Journal of Agricultural Science and Technology, 2014, 16(3):29-35.
[41]	光杨其, 宋桂成, 张金凤, 王晓楠, 唐灿明. 1个新棉花bHLH类基因GhbHLH130的克隆及表达分析[J]. 棉花学报, 2014, 26(4):363-370.doi:10.11963/cs140414.
	Guang Y Q, Song G C, Zhang J F, Wang X N, Tang C M. Molecular cloning and expression analysis of GhbHLH130,encoding a novel bHLH transcription factor in upland cotton(Gossypium hirsutum L.)[J]. Cotton science, 2014, 26(4):363-370.
[42]	高丽华, 刘博欣, 李金博, 吴燕民, 唐益雄. 陆地棉bHLH转录因子GhMYC4基因的克隆及功能分析[J], 中国农业科技导报, 2016, 18(5):33-41.doi:10.13304/j.nykjdb.2015.729.
	Gao L H, Liu B X, Li J B, Wu Y M, Tang Y X. Cloning and function analysis of bHLH transcription factor gene GhMYC4 from Gossypium hirsutism L.[J]. Journal of Agricultural Science and Technology, 2016, 18(5):33-41. doi: 10.13304/j.nykjdb.2015.729
[43]	甄军波, 蔡肖, 江振兴, 刘琳琳, 张建宏, 张香云, 迟吉娜. 棉花GhbHLH基因的克隆与表达分析[J]. 分子植物育种, 2017, 15(4):1233-1239.doi:10.13271/j.mpb.015.001233.
	Zhen J B, Cai X, Jiang Z X, Liu L L, Zhang J H, Zhang X Y, Chi J N. Molecular cloning and expression analysis of GhbHLH from upland cotton[J]. Molecular Plant Breeding, 2017, 15(4):1233-1239.
[44]	Ren W, Wang Q, Chen L, Ren Y P. Transcriptome and metabolome analyses of salt stress response in cotton(Gossypium hirsutum)seed pretreated with NaCl[J]. Agronomy, 2022, 12(8):1849.doi:10.3390/agronomy12081849.
[45]	Chen E Y, Yang X B, Liu R E, Zhang M K, Zhang M, Zhou F, Li D X, Hu H Y, Li C W. GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance[J]. Frontiers in Plant Science, 2022, 13:1019146.doi:10.3389/fpls.2022.1019146. URL
[46]	Zhang H C, Zhang W W, Jian G L, Qi F J, Si N. The genes involved in the protective effects of phytohormones in response to Verticillium dahliae infection in Gossypium hirsutum[J]. Journal of Plant Biology, 2016, 59(2):194-202.doi:10.1007/s12374-016-0568-4. URL
[47]	范强. GhCOI1和GhMYC2基因对棉花黄萎病抗性的VIGS分析[D]. 兰州: 甘肃农业大学,2017:45-46.
	Fan Q. Analysis of GhCOI1 and GhMYC2 by VIGS in the cotton resistance against Verticillium wilt[D]. Lanzhou: Gansu Agricultural University,2017:45-46.
[48]	He X, Zhu L F, Wassan G M, Wang Y J, Miao Y H, Shaban M, Hu H Y, Sun H, Zhang X L. GhJAZ2 attenuates cotton resistance to biotic stresses via the inhibition of the transcriptional activity of GhbHLH171[J]. Molecular Plant Pathology, 2018, 19(4):896-908.doi:10.1111/mpp.12575. URL
[49]	Zhou Y, Sun L Q, Wassan G M, He X, Shaban M, Zhang L, Zhu L F, Zhang X L. GbSOBIR1 confers Verticillium wilt resistance by phosphorylating the transcriptional factor GbbHLH171 in Gossypium barbadense[J]. Plant Biotechnology Journal, 2019, 17(1):152-163.doi:10.1111/pbi.12954. pmid: 29797390
[50]	赵光栋. GhbHLH122影响乙烯的生物合成进而调控棉花对枯萎病抗性的分子机制研究[D]. 泰安: 山东农业大学, 2022:38-66.doi:10.27277/d.cnki.gsdnu.2022.000010.
	Zhao G D. Molecular mechanism of GhbHLH122 affecting ethylene biosynthesis and regulating resistance to Fusarium wilt in cotton[D]. Tai'an: Shandong Agricultural University,2022:38-66.
[51]	Zhang J, Guo M Z, Wu H H, Hou L Y, Li S D, Chen G Q, Liu L, Liu Z, Lu L L, Kabir N, Yang Z R. GhPAS1,a bHLH transcription factor in upland cotton(Gossypium hirsutum),positively regulates Verticillium dahlia resistance[J]. Industrial Crops and Products, 2023, 192:116077.doi:10.1016/j.indcrop.2022.116077. URL
[52]	Ju F Y, Liu S D, Zhang S P, Ma H J, Chen J, Ge C W, Shen Q, Zhang X M, Zhao X H, Zhang Y J, Pang C Y. Transcriptome analysis and identification of genes associated with fruiting branch internode elongation in upland cotton[J]. BMC Plant Biology, 2019, 19(1):415.doi:10.1186/s12870-019-2011-8. pmid: 31590649
[53]	朱继杰, 赵红霞, 王士杰, 贾晓昀, 李妙, 王国印. 脱叶催熟剂喷施时间对不同部位棉铃发育和纤维品质的影响[J]. 棉花学报, 2023, 35(2):117-127.doi:10.11963/cs20230001.
	Zhu J J, Zhao H X, Wang S J, Jia X Y, Li M, Wang G Y. Effects of the spraying time of defoliation and ripening agent on cotton boll development and fiber quality at different fruiting branches[J]. Cotton Science, 2023, 35(2):117-127.
[54]	Wang Z J, Li Y J, Zhu Q H, Tian L W, Liu F, Zhang X Y, Sun J. Transcriptome profiling provides new insights into the molecular mechanism underlying the sensitivity of cotton varieties to mepiquat chloride[J]. International Journal of Molecular Sciences, 2022, 23(9):5043.doi:10.3390/ijms23095043. URL
[55]	Li Y Q, Qin T F, Wei C Y, Sun J L, Dong T, Zhou R Y, Chen Q J, Wang Q L. Using transcriptome analysis to screen for key genes and pathways related to cytoplasmic male sterility in cotton(Gossypium hirsutum L.)[J]. International Journal of Molecular Sciences, 2019, 20(20):5120.doi:10.3390/ijms20205120. URL
[56]	Chen E Y, Wang X Q, Gong Q, et al. A novel GhBEE1-Like gene of cotton causes anther indehiscence in transgenic Arabidopsis under uncontrolled transcription level[J]. Gene, 2017, 627:49-56.doi:10.1016/j.gene.2017.06.007. URL
[57]	Ma D, Hu Y, Yang C Q, et al. Genetic basis for glandular trichome formation in cotton[J]. Nature Communications, 2016,7:10456.doi:10.1038/ncomms10456.
[58]	刘永明, 关淑仙, 王文静, 任茂智, 李付广. 棉籽作畜禽饲料的营养价值、脱毒方法及应用研究进展[J]. 中国棉花, 2023, 50(9):35-41.doi:10.11963/cc20230076.
	Liu Y M, Guan S X, Wang W J, Ren M Z, Li F G. Nutritional value,detoxification methods,and application research of cottonseed as animal feed[J]. China Cotton, 2023, 50(9):35-41.
[59]	Min L, Hu Q, Li Y Y, Xu J, Ma Y Z, Zhu L F, Yang X Y, Zhang X L. Leafy cotyledon1-casein kinase i-tcp15-phytochrome interacting factor 4 network regulates somatic embryogenesis by regulating auxin homeostasis[J]. Plant Physiology, 2015, 169(4):2805-2821.doi:10.1104/pp.15.01480. URL
[60]	Guo H H, Guo H X, Zhang L, Fan Y J, Wu J F, Tang Z M, Zhang Y, Fan Y P, Zeng F C. Dynamic transcriptome analysis reveals uncharacterized complex regulatory pathway underlying genotype-recalcitrant somatic embryogenesis transdifferentiation in cotton[J]. Genes, 2020, 11(5):519.doi:10.3390/genes11050519. URL
[61]	Cao A P, Zheng Y Y, Yu Y, Wang X W, Shao D N, Sun J, Cui B M. Comparative transcriptome analysis of SE initial dedifferentiation in cotton of different SE capability[J]. Scientific Reports, 2017, 7(1):8583.doi:10.1038/s41598-017-08763-8. pmid: 28819177
[62]	Sun R B, Tian R P, Ma D, Wang S H, Liu C L. Comparative transcriptome study provides insights into acquisition of embryogenic ability in upland cotton during somatic embryogenesis[J]. Journal of Cotton Research, 2018, 1(1):9.doi:10.1186/s42397-018-0010-1.
[63]	Lu C, Wei Y X, Meng Z G, Liu Y M, Ali A M, Liu Q F, Abbas M, Wang Y N, Liang C Z, Wang Y, Zhang R. Overexpression of LT,an oncoprotein derived from the polyomavirus SV40,promotes somatic embryogenesis in cotton[J]. Genes, 2022, 13(5):853.doi:10.3390/genes13050853. URL
[64]	Deng J W, Sun W N, Zhang B Y, Sun S M, Xia L J, Miao Y H, He L R, Lindsey K, Yang X Y, Zhang X L. GhTCE1-GhTCEE1 dimers regulate transcriptional reprogramming during wound-induced callus formation in cotton[J]. The Plant Cell, 2022, 34(11):4554-4568.doi:10.1093/plcell/koac252. pmid: 35972347

选择文件类型/文献管理软件名称

选择包含的内容