华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 133-140. doi: 10.7668/hbnxb.20195360

所属专题: 棉花 植物保护 生物技术

• 资源环境·植物保护 • 上一篇    下一篇

棉花抗枯萎病基因GhERF14的功能分析

赵龙飞1,2, 张建华3, 赵曾强4, 蔡树东2, 张薇1,   

  1. 1 石河子大学 农学院,新疆 石河子 832000
    2 哈密市畜牧工作站,新疆 哈密 839000
    3 河北科技师范学院 园艺科技学院,河北 昌黎 066600
    4 新疆农垦科学院 棉花研究所,新疆 石河子 832000
  • 收稿日期:2024-11-19 出版日期:2025-06-28
  • 通讯作者:
    张 薇(1969—),女,江苏镇江人,教授,博士,主要从事棉花分子育种研究。
  • 作者简介:

    赵龙飞(1994—),男,河北保定人,畜牧师,硕士,主要从事农业、畜牧业研究。

  • 基金资助:
    国家自然科学基金项目(31260358)

Functional Analysis of Fusarium wilt Resistance Gene GhERF14 in Cotton

ZHAO Longfei1,2, ZHANG Jianhua3, ZHAO Zengqiang4, CAI Shudong2, ZHANG Wei1,   

  1. 1 Agriculture College,Shihezi University,Shihezi 832000,China
    2 Hami Animal Husbandry Station,Hami 839000,China
    3 College of Horticultural Science & Technology,Hebei Normal University of Science & Technology,Changli 066600,China
    4 Cotton Research Institute of Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832000,China
  • Received:2024-11-19 Published:2025-06-28

摘要:

探究棉花GhERF14基因与尖孢镰刀菌致病力的关系,解析尖孢镰刀菌致病分子机制,初步探究棉花GhERF14基因对枯萎病的响应及对相关抗病基因的调控作用,为培育抗枯萎病的棉花新品种提供一定的理论依据。利用基因克隆、病毒诱导基因沉默(VIGS)构建了GhERF14 基因非保守结构域干扰载体pTRV2-GhERF14,通过实时荧光定量(qRT-PCR)技术和VIGS技术,研究枯萎病菌胁迫和激素处理后,GhERF14和下游与木质素(Lignin)、乙烯(ET)、茉莉酸(JA)、水杨酸(SA)相关基因,抗氧化酶基因及病程相关蛋白(PR)基因的表达特征,分析其在棉花抗病过程中的作用,表明抑制GhERF14基因的表达可显著降低茉莉酸、水杨酸以及乙烯合成及信号通路相关基因的表达。利用VIGS 技术将 GhERF14 基因沉默后,棉株对枯萎病菌更敏感,表明GhERF14在尖孢镰刀菌致病及宿主-病原体互作过程中可能发挥着重要的作用。

关键词: 棉花, GhERF14, 枯萎病, VIGS, 尖孢镰刀菌

Abstract:

This study explored the relationship between cotton GhERF14 gene and Fusarium oxysporum pathogenicity,analyzed the molecular mechanism of F.oxysporum pathogenicity,and tentatively explored the response of cotton GhERF14 gene to Fusarium wilt disease and its regulatory effect on related resistance genes,to provides some theoretical basis for breeding new cotton cultivars resistant to wilt.Gene cloning and virus-induced gene silencing(VIGS)were used to construct the non-conserved domain interference vector pTRV2-GhERF14.Using Real-time fluorescence quantification(qRT-PCR)technology and VIGS technology,the expression characteristics of GhERF14 and downstream genes related to lignin,ethylene(ET),jasmonic acid(JA),salicylic acid(SA),antioxidant enzymes and disease progression-related protein(PR)were analyzed after F.oxysporum stress and hormone treatment,and the role of GhERF14 in the process of cotton disease resistance was analyzed.The results indicated that inhibition of GhERF14 gene expression could significantly reduce the synthesis of jasmonic acid(JA),salicylic acid(SA)and ethylene(ET)and the expression of genes related to the signaling pathway.After GhERF14 gene silencing by VIGS technology,cotton plants were more susceptible to Fusarium wilt.These results suggested that GhERF14 may play an important role in the pathogenesis and host-pathogen interaction of F.oxysporum.

Key words: Cotton, GhERF14, Blight, VIGS, Fusarium oxysporum

中图分类号: 

引用本文

赵龙飞, 张建华, 赵曾强, 蔡树东, 张薇. 棉花抗枯萎病基因GhERF14的功能分析[J]. 华北农学报, 2025, 40(3): 133-140. doi: 10.7668/hbnxb.20195360.

ZHAO Longfei, ZHANG Jianhua, ZHAO Zengqiang, CAI Shudong, ZHANG Wei. Functional Analysis of Fusarium wilt Resistance Gene GhERF14 in Cotton[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 133-140. doi: 10.7668/hbnxb.20195360.