摘要： TCP家族成员是植物特有的转录因子,在植物生长、代谢活动及逆境应答等关键生物过程中发挥不可或缺的作用。为了解TCP基因家族在花魔芋基因组中的数量、分布和表达等情况,利用生物信息学技术对AkTCP家族基因进行鉴定,分析该家族成员的理化性质、亚细胞定位、共线性、基因结构及进化关系,采用qRT-PCR技术分析响应生物、非生物胁迫的基因表达情况。结果表明,花魔芋全基因组中鉴定出30个TCP家族成员,其蛋白包含135~562个氨基酸残基,分子质量为15.08~57.20 ku,等电点为4.96~11.49,大部分为碱性蛋白,均为不稳定的亲水性蛋白,主要分布在细胞核上,其基因在染色体上不均匀分布。AkTCP共线性基因仅在花魔芋8条染色体中分布,包含4个染色体间共线性事件和5个染色体内共线性事件。AkTCP基因结构较为简单,大部分不含内含子,均含有高度保守的TCP结构域,可分为Class Ⅰ和Class Ⅱ 2个组别,Class Ⅱ又分为CIN和CYC/TB1 2个亚类。启动子顺式作用元件分析结果显示,AkTCP启动子上含有多个生理响应、光响应、植物激素和逆境胁迫响应等元件。qRT-PCR分析结果表明,AkTCP家族成员参与了干旱胁迫、盐胁迫、茉莉酸甲酯和软腐病原菌侵染应答过程并作出积极响应。

关键词: 花魔芋, TCP基因家族, 转录因子, 表达分析

Abstract:

The TCP transcription factor family is unique to plants and plays a crucial role in key biological processes such as plant growth,metabolic activities,and stress responses.To investigate the quantity,distribution and expression patterns of TCP gene family in the Amorphophallus konjac genome,bioinformatics approaches were utilized to identify the AkTCP gene family,followed by a comprehensive analysis of the physicochemical properties,subcellular localization,collinearity,gene structure,and evolutionary relationships.Additionally,gene expression in response to biotic and abiotic stresses was examined by qRT-PCR.The findings were summarized as follows:the A.konjac genome contained 30 AkTCP family genes,with protein lengths ranging from 135 to 562 amino acids.These proteins had molecular weights between 15.08 and 57.20 ku,and isoelectric points between 4.96 and 11.49.Most of these were basic,hydrophilic,and unstable proteins,predominantly localized in the nucleus.These genes were unevenly distributed across the chromosomes.The collinear AkTCP genes were unevenly distributed across eight chromosomes,comprising four inter-chromosomal and five intrachromosomal collinearity events.The AkTCP gene structure was relatively simple,with most genes lacking introns,and all AkTCP transcription factors exhibit a highly conserved TCP domain.Phylogenetic classification separates the 30 AkTCP genes into two major classes,Class Ⅰ and Class Ⅱ,with Class Ⅱ further subdivided into the CIN and CYC/TB1 subclasses.The promoters of AkTCP genes contain cis-elements related to physiological response,light-responsive,phytohormone-responsive and stress-responsive elements.The results of qRT-PCR analysis indicated that AkTCP family members were involved in and responded positively to drought stress, salt stress, methyl jasmonate and soft rot pathogens of A. konjac.

Key words: Amorphophallus konjac, TCP gene family, Transcription factor, Expression analysis

中图分类号: 

• S632.3