小麦E3泛素连接酶TaSINA101的抗逆相关功能研究

doi:10.7668/hbnxb.20195704

摘要/Abstract

摘要：

泛素化途径是植物响应干旱胁迫的关键信号途径之一。为明确E3泛素连接酶TaSINA101基因响应干旱胁迫的生物学功能,以小麦抗旱优异品种晋麦47为材料,克隆TaSINA61、TaSINA101和TaSINA105基因,利用生物信息学手段分析其序列特征,通过qRT-PCR检测3个基因在PEG-6000、NaCl、低温和ABA处理下小麦根和叶中表达量的变化。通过转基因水稻异源表达TaSINA101,分析TaSINA101响应干旱胁迫过程中的生物学功能。结果表明,TaSINA61、TaSINA101、TaSINA105基因均包含1个内含子和2个外显子,编码的蛋白质均由282个氨基酸组成,启动子区主要包含生长发育调控元件、激素响应元件和逆境胁迫响应元件。qRT-PCR表达分析显示,这3个基因在根和叶中均受干旱胁迫等各种非生物胁迫诱导表达。干旱胁迫处理TaSINA101转基因水稻的表型分析发现,转基因水稻株系OE-1、OE-2和OE-3叶片的鲜质量及干质量、最大根长、根系平均直径和叶片相对含水量各项指标均显著低于野生型,而转基因水稻株系OE-1、OE-2叶片相对电导率则显著高于野生型。因此,TaSINA101负调控水稻的干旱胁迫耐受性,为深入解析小麦TaSINA101基因的生物学功能提供了依据。

关键词: 小麦, 干旱胁迫, E3泛素连接酶, 表达分析, 功能研究, TaSINA101

Abstract:

The ubiquitination pathway is one of the key signaling pathways in response to drought stress.In order to clarify the biological function of E3 ubiquitin ligase TaSINA101 gene in response to drought stress,the TaSINA61,TaSINA101 and TaSINA105 genes were cloned from JM47,an excellent drought-resistant wheat cultivar,and their sequence characteristics were analyzed by bioinformatics methods,and the expression levels of the three genes in wheat roots and leaves were detected by qRT-PCR under PEG-6000,NaCl,low temperature and ABA treatments.The heterologous expression of TaSINA101 in transgenic rice was used to analyze the biological function of TaSINA101 in response to drought stress.The results showed that the TaSINA61,TaSINA101 and TaSINA105 genes contained one intron and two exons,and the encoded proteins were composed of 282 amino acids.The qRT-PCR expression analysis showed that the expression of these three genes was induced by various abiotic stresses such as drought stress in roots and leaves.Phenotypic analysis of TaSINA101 transgenic rice under drought stress showed that the leaf fresh weight and dry weight, maximum root length, average root diameter and leaf relative water content of transgenic rice lines OE-1, OE-2 and OE-3 were significantly lower than those of wild type,while the relative conductivity of leaves of transgenic rice lines OE-1 and OE-2 was significantly higher than that of wild type.Therefore,TaSINA101 negatively regulates drought stress tolerance in rice.This study provides a basis for in-depth analysis of the biological function of TaSINA101 gene in wheat.

Key words: Wheat, Drought stress, E3 ubiquitin ligase, Expression analysis, Functional studies, TaSINA101

中图分类号:

刘雅鑫, 陈涛, 高维东, 郭利建, 车卓, 杨德龙. 小麦E3泛素连接酶TaSINA101的抗逆相关功能研究[J]. 华北农学报, 2025, 40(4): 19-28. doi: 10.7668/hbnxb.20195704.

LIU Yaxin, CHEN Tao, GAO Weidong, GUO Lijian, CHE Zhuo, YANG Delong. Study on Stress-related Function of Wheat E3 Ubiquitin Ligase TaSINA101[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 19-28. doi: 10.7668/hbnxb.20195704.

http://www.hbnxb.net/CN/Y2025/V40/I4/19

图/表 8

表1 基因克隆和荧光定量PCR引物序列

Tab.1 Gene cloning and fluorescent quantitative PCR primer sequence

引物名称 Primer name	引物序列(5'-3') Primer sequence (5'-3')
TaSINA61-F	ATGGACGGGGGCACTACTAG
TaSINA61-R	TACACTGCGTACTGGTTTCAGAC
TaSINA101-F	ATGGATGGTGCTACCTCTAGCAAG
TaSINA101-R	TACACCGCGTATTGGTTTCAG
TaSINA105-F	ATGGATTGTGGTACCTCTAGCAAG
TaSINA105-R	TACACCGCGTACTGGTTTCAG
qRT-TaSINA61-F	GGCAAGAGGCAGAATGCAAC
qRT-TaSINA61-R	CGCTCGGGCATTCCTTCT
qRT-TaSINA101-F	CGAAGCTCATCGTTGTCTGA
qRT-TaSINA101-R	TACACCGCGTATTGGTTTCA
qRT-TaSINA105-F	TTAAGTTCACCCTAGAACCAGG
qRT-TaSINA105-R	GGGCTCATGCACCTTTACTTTAG
TaACTIN-F	GCCGTGCTTTCCCTCTATG
TaACTIN-R	GCTTCTCCTTGATGTCCCTTA

图1 TaSINA61、TaSINA101和TaSINA105基因的克隆 M.DL 2000 DNA Marker;1.TaSINA61目的片段;2.TaSINA101目的片段;3.TaSINA105目的片段。

Fig.1 Cloning of TaSINA61, TaSINA101, and TaSINA105 genes M.DL 2000 DNA Marker; 1. TaSINA61 target fragment;2.TaSINA101 target fragment;3.TaSINA105 target fragment.

图2 TaSINA61、TaSINA101、TaSINA105基因序列比对

Fig.2 Sequence alignment of TaSINA61, TaSINA101 and TaSINA105 genes

图3 TaSINA61、TaSINA101和TaSINA105基因结构及其编码蛋白结构分析 A:TaSINA61、TaSINA101和TaSINA105基因结构示意图;B:TaSINA61、TaSINA101和TaSINA105结构域预测示意图;C:红色矩形框.RING-Ubox superfamily结构域,绿色矩形框.SINA结构域。

Fig.3 Analysis of TaSINA61,TaSINA101 and TaSINA105 gene sequence and protein structure A:Schematic diagram of TaSINA61, TaSINA101 and TaSINA105 gene structures; B:Schematic diagram of TaSINA61,TaSINA101 and TaSINA105 domain prediction; C:The red rectangular box.RING-Ubox superfamily domain, The green rectangular box.SINA domain.

表2 基因信息及其编码蛋白的理化性质分析

Tab.2 Gene information and protein physicochemical properties analysis

基因 Gene	基因号 Gene ID number	物理位置/bp Physical position	分子质量/ku Molecular weight	等电点 pI	蛋白长度/aa Protein length
TaSINA61	TraesCS3A02G030600	16 620 265-16 622 563	31.251 87	8.21	282
TaSINA101	TraesCS3B02G052800	26 746 166-26 748 742	31.435 24	8.03	282
TaSINA105	TraesCS3D02G029000	10 458 906-10 461 617	31.308 19	8.34	282

图4 TaSINA61、TaSINA101和TaSINA105启动子顺式作用元件

Fig.4 The cis-acting element of the TaSINA61,TaSINA101 and TaSINA105 promoter

图5 盐、干旱、低温和ABA处理下TaSINA61、TaSINA101和TaSINA105基因表达模式不同字母表示0.05水平差异显著性(P<0.05)。图5同。

Fig.5 Expression patterns of TaSINA61,TaSINA101 and TaSINA105 genes under salt, drought, low temperature and ABA treatment Different letters represent significant difference at the 0.05 level(P<0.05).The same as Fig.5.

图6 TaSINA101过表达水稻株系干旱胁迫下的表型分析

Fig.6 Phenotype of TaSINA101 overexpressed rice lines under drought stress

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