氨基酸透性酶基因<i>OsAAP8</i>超表达对水稻生长发育及其稻米品质的影响

doi:10.7668/hbnxb.20195799

华北农学报 ›› 2025, Vol. 40 ›› Issue (5): 26-34. doi: 10.7668/hbnxb.20195799

所属专题： 水稻；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响

彭波¹, 刘岩¹, 徐茹欣¹, 徐海芳¹, 彭娟², 赵强³, 张巧玉⁴, 黄雅琴⁴, 张志国⁵, 申关望⁶, 祁玉良⁶, 汪全秀¹, 孙艳芳¹

¹ 信阳师范大学生命科学学院, 河南信阳 464000
² 信阳市植保植检站, 河南信阳 464000
³ 河南省科研平台服务中心, 河南郑州 450003
⁴ 信阳农林学院, 河南信阳 464100
⁵ 河南羚锐制药股份有限公司, 河南信阳 465550
⁶ 信阳市农业科学院, 河南信阳 464000

收稿日期:2025-01-21 出版日期:2025-11-03
作者简介:
彭波(1980—),男,河南信阳人,副教授,博士,主要从事水稻遗传育种研究。
基金资助:
国家自然科学基金项目(U2004141); 国家自然科学基金项目(12305399); 河南省科技研发计划联合基金(应用攻关)项目(242103810019); 河南省科技攻关项目(242102110266); 河南省科技攻关项目(242102110270); 河南省科技攻关项目(231111110500); 信阳市科技攻关项目(20230025); 信阳师范大学大学生科研基金项目(202410477040)

Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality

PENG Bo¹, LIU Yan¹, XU Ruxin¹, XU Haifang¹, PENG Juan², ZHAO Qiang³, ZHANG Qiaoyu⁴, HUANG Yaqin⁴, ZHANG Zhiguo⁵, SHEN Guanwang⁶, QI Yuliang⁶, WANG Quanxiu¹, SUN Yanfang¹

¹ College of Life Sciences, Xinyang Normal University, Xinyang 464000, China
² Xinyang Plant Protection and Inspection Station, Xinyang 464000, China
³ Henan Scientific Research Platform Service Center, Zhengzhou 450003, China
⁴ Xinyang Agriculture and Forestry University, Xinyang 464100, China
⁵ Henan Lingrui Pharmaceutical Company Limited, Xinyang 465550, China
⁶ Xinyang City Academy of Agricultural Sciences, Xinyang 464000, China

Received:2025-01-21 Published:2025-11-03

摘要/Abstract

摘要：

以粳稻品种中花11为背景,构建OsAAP8超表达转基因株系,通过对其农艺性状和品质性状进行检测分析,探究OsAAP8超表达对水稻生长发育和稻米品质的影响,为利用OsAAP8进行优质高产水稻新品种的分子设计育种提供理论依据。结果显示,与转基因阴性对照植株相比,OsAAP8超表达转基因阳性植株的株高显著降低、分蘖数显著减少,单株产量显著降低。品质性状检测结果显示,OsAAP8超表达转基因阳性稻米中谷氨酸含量、苏氨酸含量、必需氨基酸含量、总氨基酸含量、蛋白质含量、直链淀粉含量、胶稠度和糊化温度均显著升高,总淀粉含量和糙米率没有显著变化,游离脂肪酸含量、食味值、精米率和整精米率显著降低。光学显微镜和扫描电镜观察结果显示,OsAAP8超表达转基因阳性稻米的垩白率和垩白度显著升高,垩白面积没有显著变化,淀粉粒的形状和排列方式没有发生明显改变。粒型检测结果显示,OsAAP8超表达转基因阳性稻米的粒长、粒宽和粒厚均未发生显著变化。以上结果表明,OsAAP8超表达不利于水稻生长发育,但能够显著改善稻米的营养品质,这为高品质水稻新品种培育提供重要信息。

关键词: 水稻, 氨基酸透性酶, OsAAP8, 稻米品质, 超表达

Abstract:

OsAAP8 overexpressed transgenic lines were constructed based on the japonica rice variety Zhonghua 11.By detecting and analyzing its agronomic and quality traits,it explored the effects of OsAAP8 overexpression on rice growth and development,as well as rice quality,to provide theoretical basis for molecular design breeding by utilizing OsAAP8 gene to improve rice quality and yield.The results showed that compared with the transgenic negative plants,OsAAP8 overexpressed transgenic positive plants had significantly reduced plant height,tiller number,and single plant yield.The quality trait testing results showed that the content of glutamic acid,threonine,essential amino acids,total amino acids,protein,amylose,gel consistency,and gelatinization temperature in OsAAP8 overexpressed transgenic positive rice were significantly increased.The content of total starch and brown rice rate did not change significantly.The content of free fatty acids,taste value,polished rice rate,and whole polished rice rate were significantly decreased.The observation results of optical microscope and scanning electron microscope showed that the chalkiness rate and chalkiness degree of OsAAP8 overexpressed transgenic positive rice were significantly increased,but the chalkiness area did not change significantly,and the shape and arrangement of starch granules did not change.The grain size detection results showed no significant changes in grain length,width,and thickness of OsAAP8 overexpressed transgenic positive rice.The above results indicate that overexpression of OsAAP8 is not conducive to the growth and development of rice,but can significantly improve the nutritional quality of rice,which provides important information for the cultivation of new high-quality rice varieties.

Key words: Rice, Amino acid permease, OsAAP8, Rice quality, Overexpression

中图分类号:

S511稻

彭波, 刘岩, 徐茹欣, 徐海芳, 彭娟, 赵强, 张巧玉, 黄雅琴, 张志国, 申关望, 祁玉良, 汪全秀, 孙艳芳. 氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响[J]. 华北农学报, 2025, 40(5): 26-34. doi: 10.7668/hbnxb.20195799.

PENG Bo, LIU Yan, XU Ruxin, XU Haifang, PENG Juan, ZHAO Qiang, ZHANG Qiaoyu, HUANG Yaqin, ZHANG Zhiguo, SHEN Guanwang, QI Yuliang, WANG Quanxiu, SUN Yanfang. Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 26-34. doi: 10.7668/hbnxb.20195799.

http://www.hbnxb.net/CN/Y2025/V40/I5/26

图/表 7

表1 引物序列

Tab.1 Primer sequences

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
hpt-F	ACACTACATGGCGTGATTTCAT
hpt-R	TCCACTATCGGCGAGTACTTCT
β-actin-F	TGCTATGTACGTCGCCATCCAG
β-actin-R	AATGAGTAACCACGCTCCGTCA
OsAAP8-F	CAACGTCTCCAGCGTGCCCTAC
OsAAP8-R	GGCTTCCCTTCAACCCTCC

图1 OsAAP8超表达转基因阳性植株鉴定 A.OsAAP8超表达植株阳性鉴定:Marker.2 000 bp;B.OsAAP8表达水平检测。不同字母表示差异显著(P<0.05)。图2—6同。

Fig.1 Identification of OsAAP8 overexpressed transgenic positive plants A.Positive identification of OsAAP8 overexpressed plants:Marker.2 000 bp; B.OsAAP8 expression level detection. Different letters indicate significant difference(P<0.05).The same as Fig.2—6.

图2 OsAAP8超表达转基因植株农艺性状检测分析 A.OsAAP8超表达转基因株系的表型观察,标尺为15 cm。

Fig.2 Detection and analysis of agronomic traits in OsAAP8 overexpressed transgenic plants A.Phenotypic observation of OsAAP8 overexpressed transgenic lines,the scale is 15 cm.

图3 OsAAP8超表达转基因稻米营养品质性状检测分析

Fig.3 Detection and analysis of nutritional quality traits of OsAAP8 overexpressed transgenic rice

图4 OsAAP8超表达转基因稻米垩白性状检测分析 A.垩白性状统计分析;B.垩白的光学显微镜观察(40×);C.垩白部位扫描电镜观察;D.非垩白部位扫描电镜观察。

Fig.4 Detection and analysis of chalkiness traits in OsAAP8 overexpressed transgenic rice A.Statistical analysis of chalkiness traits;B.Observation of chalkiness under optical microscope(40×);C.Scanning electron microscopy observation of chalky areas;D.Scanning electron microscopy observation of non chalky areas.

图5 OsAAP8超表达转基因稻米粒型检测分析

Fig.5 Detection and analysis of grain size in OsAAP8 overexpressed transgenic rice

图6 OsAAP8超表达转基因稻米的蒸煮食味品质和加工品质检测分析

Fig.6 Detection and analysis of cooking taste quality and processing quality of OsAAP8 overexpressed transgenic rice

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氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响

Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality

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氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响

Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality

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