娘亚牦牛<i>BACE2</i>基因多态性与生长性状的关联性分析

doi:10.7668/hbnxb.20195661

摘要/Abstract

摘要：

旨在探究BACE2基因中的单核苷酸多态性(SNPs)与娘亚牦牛生长性状之间的相关性,为改善牦牛的生长性能提供潜在的分子标记位点。基于重测序数据,在娘亚牦牛BACE2基因中检测到2个SNP位点(g.47,411T>C和g.81,108T>C),对上述的2个SNPs进行遗传多态性分析,并且分析这2个SNP位点与牦牛生长性状之间的相关性。遗传多态性分析发现,g.47,411T>C和g.81,108T>C位点为低度多态位点。关联结果表明,g.47,411T>C的TT基因型牦牛个体的体质量和胸围性状显著高于CC型牦牛个体,且TT基因型牦牛个体的体长显著高于TC和CC型牦牛个体。g.81,108T>C位点的TT基因型牦牛个体的体质量和胸围显著高于CC型牦牛个体,TT基因型的牦牛个体的体长性状显著高于TC型牦牛个体,且TC型牦牛个体的体长显著高于CC型牦牛个体。综上所述,BACE2基因g.47,411T>C和g.81,108T>C位点对娘亚牦牛的生长性状具有显著影响。

关键词: 娘亚牦牛, BACE2基因, SNP, 基因多态性, 生长性状

Abstract:

This study aimed to investigate the association between single-nucleotide polymorphisms(SNPs)in BACE2 gene and growth traits of Niangya yak,and to provide potential molecular markers for improving the growth performance of yak.Based on the re-sequencing data,two SNP sites(g.47,411T>C and g.81,108T>C)were detected in the BACE2 gene of Niangya yak.The genetic polymorphism of the two SNPs and the correlation between the two SNPs and growth traits of yaks were analyzed.Genetic polymorphism analysis showed that g.47,411T>C and g.81,108T>C loci were low polymorphic loci.The correlation results showed that the body weight and chest circumference traits of TT yaks with g.47,411T>C were significantly higher than those of CC yaks,and the body length of TT yaks was significantly higher than that of TC and CC yaks.The body weight and chest circumference of TT yaks with g.81,108T>C locus were significantly higher than those of CC yaks.The body length of TT yaks was significantly higher than that of TC yaks,and the body length of TC yaks was significantly higher than that of CC yaks.In conclusion,the g.47,411T>C and g.81,108T>C loci of the BACE2 gene had significant effects on growth traits of Niangya yak.

Key words: Niangya yak, BACE2 gene, SNP, Genetic polymorphism, Growth trait

中图分类号:

S823.85

张娟香, 任稳稳, 余道宁, 徐业芬, 欧杰次仁, 梁春年. 娘亚牦牛BACE2基因多态性与生长性状的关联性分析[J]. 华北农学报, 2025, 40(S1): 307-312. doi: 10.7668/hbnxb.20195661.

ZHANG Juanxiang, REN Wenwen, YU Daoning, XU Yefen, OJIE Ciren, LIANG Chunnian. Correlation Analysis Between BACE2 Gene Polymorphism and Growth Traits in Niangya Yak[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 307-312. doi: 10.7668/hbnxb.20195661.

http://www.hbnxb.net/CN/Y2025/V40/IS1/307

图/表 5

参考文献 29

[1]	倪彬, 朱莉, 高乐程, 尼玛仓决, 普布曲珍, 苟潇, 孔小艳, 巴桑珠扎. 牦牛高原适应的生理与遗传分子机制[J]. 黑龙江畜牧兽医, 2023(23):13-20,130.doi:10.13881/j.cnki.hljxmsy.2023.02.0052.
	Ni B, Zhu L, Gao L C, Ni M C J, Pu B Q Z, Gou X, Kong X Y, Kong X Y, Ba S Z Z. Physiological and genetic molecular mechanisms of plateau adaptation in the yak[J]. Heilongjiang Animal Science and Veterinary Medicine, 2023(23):13-20,130.
[2]	张小建, 王蕊, 张重庆, 李键. 论牦牛的产业化与民族经济发展[J]. 四川畜牧兽医, 2007, 34(4):31-32,36.doi:10.3969/j.issn.1001-8964.2007.04.015.
	Zhang X J, Wang R, Zhang C Q, Li J. On yak industrialization and national economic development[J]. Sichuan Animal & Veterinary Sciences, 2007, 34(4):31-32,36.
[3]	Guo X S, Long R J, Kreuzer M, Ding L M, Shang Z H, Zhang Y, Yang Y, Cui G X. Importance of functional ingredients in yak milk-derived food on health of Tibetan nomads living under high-altitude stress:a review[J]. Critical Reviews in Food Science and Nutrition, 2014, 54(3):292-302.doi:10.1080/10408398.2011.584134. URL
[4]	Garcia A O, Otto P I, Glatzl L A Jr, de Fátima Bretanha Rocha R, dos Santos M G, de Oliveira D A, da Silva M V G B, do Carmo Panetto J C, Machado M A, da Silva Verneque R, Guimarães S E F. Pedigree reconstruction and population structure using SNP markers in Gir cattle[J]. Journal of Applied Genetics, 2023, 64(2):329-340.doi:10.1007/s13353-023-00747-x.
[5]	王可, 祝超智, 赵改名, 郝婉名, 田玮, 参木友. 中国牦牛的品种与分布[J]. 中国畜牧杂志, 2019, 55(10):168-171.doi:10.19556/j.0258-7033.20190225-02.
	Wang K, Zhu C Z, Zhao G M, Hao W M, Tian W, Can M Y. Variety and distribution of yak in China[J]. Chinese Journal of Animal Science, 2019, 55(10):168-171.
[6]	Johnsson M. Genomics in animal breeding from the perspectives of matrices and molecules[J]. Hereditas, 2023, 160(1):20.doi:10.1186/s41065-023-00285-w. pmid: 37149663
[7]	张振宇, 梁春年. 分子育种技术在牦牛育种中的应用[J]. 中国畜禽种业, 2024, 20(10):31-41.doi:10.3969/j.issn.1673-4556.2024.10.005.
	Zhang Z Y, Liang C N. Application of molecular breeding technology in yak breeding[J]. The Chinese Livestock and Poultry Breeding, 2024, 20(10):31-41.
[8]	徐宇辉, 吴志鹏, 盛欣, 李瑞哲, 马志杰. 牦牛全基因组关联研究进展[J]. 中国畜牧杂志, 2024, 60(12):41-47.doi:10.19556/j.0258-7033.20231221-10.
	Xu Y H, Wu Z P, Sheng X, Li R Z, Ma Z J. Research progress on genome-wide association study(GWAS)in yak[J]. Chinese Journal of Animal Science, 2024, 60(12):41-47.
[9]	La Y F, Ma F L, Ma X M, Bao P J, Chu M, Liang C N, Guo X, Yin M C, Li J Y, Yan P. Different expression of LHR,PRLR,GH and IGF1 during testicular development of yak[J]. Reproduction in Domestic Animals, 2022, 57(2):221-227.doi:10.1111/rda.14044. URL
[10]	Li X M, Hong L, Coughlan K, Wang L, Cao L, Tang J. Structure-activity relationship of memapsin 2:implications on physiological functions and Alzheimer's disease[J]. Acta Biochimica et Biophysica Sinica, 2013, 45(8):613-621.doi:10.1093/abbs/gmt050. URL
[11]	Vassar R, Bennett B D, Babu-Khan S, Kahn S, Mendiaz E A, Denis P, Teplow D B, Ross S, Amarante P, Loeloff R, Luo Y, Fisher S, Fuller J, Edenson S, Lile J, Jarosinski M A, Biere A L, Curran E, Burgess T, Louis J C, Collins F, Treanor J, Rogers G, Citron M. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE[J]. Science, 1999, 286(5440):735-741.doi:10.1126/science.286.5440.735. pmid: 10531052
[12]	Díaz-Catalán D, Alcarraz-Vizán G, Castaño C, de Pablo S, Rodríguez-Comas J, Fernández-Pérez A, Vallejo M, Ramírez S, Claret M, Parrizas M, Novials A, Servitja J M. BACE2 suppression in mice aggravates the adverse metabolic consequences of an obesogenic diet[J]. Molecular Metabolism, 2021, 53:101251.doi:10.1016/j.molmet.2021.101251.
[13]	Walter J. Fishing for function distinct roles of Bace1 and Bace2 in Zebrafish development[J]. Journal of Neurochemistry, 2013, 127(4):435-437.doi:10.1111/jnc.12200. URL
[14]	Guan D F, Sun S Y, Song L Y, Zhao P P, Nie Y G, Huang X, Zhou W L, Yan L, Lei Y H, Hu Y B, Wei F W. Taking a color photo:a homozygous 25-bp deletion in Bace2 may cause brown-and-white coat color in giant pandas[J]. Proceedings of the National Academy of Sciences of the United States of America, 2024, 121(11):e2317430121.doi:10.1073/pnas.2317430121.
[15]	黄彩霞, 高媛, 孙宝忠, 卢凌. 牦牛品种品质研究进展[J]. 肉类研究, 2012, 26(9):30-34.
	Huang C X, Gao Y, Sun B Z, Lu L. Research progress in meat quality of different yak breeds[J]. Meat Research, 2012, 26(9):30-34.
[16]	杨树猛, 赵光平, 牟永娟, 张海滨, 才让闹日, 李鹏霞, 赵元芳, 宫玉霞, 祁红霞. 甘南牦牛产业高质量发展存在的问题及建议[J]. 甘肃畜牧兽医, 2023, 53(6):93-96.doi:10.15979/j.cnki.cn62-1064/s.2023.06.028.
	Yang S M, Zhao G P, Mu Y J, Zhang H B, Cai R, Li P X, Zhao Y F, Gong Y X, Qi H X. Problems and suggestions on high-quality development of Gannan yak industry[J]. Gansu Animal Husbandry and Veterinary Medicine, 2023, 53(6):93-96.
[17]	郑菲, 黄志, 王婧尊, 祁高展, 徐红伟, 高丹丹, 郭鹏辉, 曹忻, 杨具田, 蔡勇. 绵羊MTPN基因多态性与生长性状的关联分析[J]. 中国畜禽种业, 2024, 20(4):39-47.doi:10.3969/j.issn.1673-4556.2024.04.006.
	Zheng F, Huang Z, Wang J Z, Qi G Z, Xu H W, Gao D D, Guo P H, Cao X, Yang J T, Cai Y. Association between MTPN gene polymorphism and growth traits in sheep[J]. The Chinese Livestock and Poultry Breeding, 2024, 20(4):39-47.
[18]	Ren W W, Ma X M, Yu D N, Wu X Y, La Y F, Guo X, Chu M, Yan P, Lan X Y, Liang C N. Polymorphisms of KCNJ6 gene and their correlation with immune indicators in yaks(Bos grunniens)[J]. Biomolecules, 2024, 14(12):1576.doi:10.3390/biom14121576. URL
[19]	杨旻奇, 黄巧艳, 韩笑, 伍柏仰, 乌兰·合得尔, 邢凤. 多浪羊ESR1基因多态性与产羔性状的关联性分析[J]. 中国草食动物科学, 2025, 45(4):96-101.doi:10.3969/j.issn.2095-3887.2024.00.111.
	Yang M Q, Huang Q Y, Han X, Wu B Y, Wulan H D E, Xing F. Association analysis of ESR1 gene polymorphisms and lambing traits in Duolang sheep[J]. China Herbivore Science, 2025, 45(4):96-101.
[20]	马小燕, 王文文, 郑凤玲, 刘汇鑫, 许飞, 陈伟, 曾勇庆, 唐辉. 江泉黑猪PLAG1基因多态性及其与生长性能的关联分析[J]. 农业生物技术学报, 2024, 32(7):1564-1576.doi:10.3969/j.issn.1674-7968.2024.07.009.
	Ma X Y, Wang W W, Zheng F L, Liu H X, Xu F, Chen W, Zeng Y Q, Tang H. PLAG1 gene polymorphism and its association analysis with growth performance in Jiangquan black pigs(Sus scrofa)[J]. Journal of Agricultural Biotechnology, 2024, 32(7):1564-1576.
[21]	黄纯, 阎萍, 梁春年. 中国牦牛种业现状与发展方向[J]. 中国畜禽种业, 2023, 19(7):121-127.doi:10.3969/j.issn.1673-4556.2023.07.019.
	Huang C, Yan P, Liang C N. Present situation and development direction of yak seed industry in China[J]. The Chinese Livestock and Poultry Breeding, 2023, 19(7):121-127.
[22]	邢生炎, 黄永震, 吕世杰, 王二耀, 雷初朝, 梁莎. 生物育种技术及其在畜禽育种中的应用研究进展[J]. 中国畜牧杂志, 2024, 60(3):57-65.doi:10.19556/j.0258-7033.20230505-01.
	Xing S Y, Huang Y Z, Lyu S J, Wang E Y, Lei C Z, Liang S. Research progress on biological breeding technology and its application in livestock and poultry breeding[J]. Chinese Journal of Animal Science, 2024, 60(3):57-65.
[23]	丁维芹, 祁增源, 刘亚倩, 韩银仓, 李文浩, 孙永刚. 青海高原型牦牛GHR基因多态性与生长发育的关联性[J]. 西北农林科技大学学报(自然科学版), 2024, 52(1):9-15,35.doi:10.13207/j.cnki.jnwafu.2024.01.002.
	Ding W Q, Qi Z Y, Liu Y Q, Han Y C, Li W H, Sun Y G. Correlation between GHR gene polymorphism and growth traits in Qinghai plateau yaks[J]. Journal of Northwest A & F University(Natural Science Edition), 2024, 52(1):9-15,35.
[24]	周建强, 司思, 祁增源, 韩银仓, 刘秀, 孙永刚. 牦牛SMAD1基因第1外显子SNPs检测及与生长性状的关联分析[J]. 西北农业学报, 2023, 32(2):179-186.doi:10.7606/j.issn.1004-1389.2023.02.002.
	Zhou J Q, Si S, Qi Z Y, Han Y C, Liu X, Sun Y G. Detection of SNPs in exon 1 of SMAD1 gene and its association with growth traits in yak[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2023, 32(2):179-186.
[25]	孟光耀. 阿什旦牦牛生长曲线的拟合及生长性状与FOXO1和THRSP基因的关联分析[D]. 兰州: 中国农业科学院, 2023.doi:10.27630/d,cnki.gznky.2023.000678.
	Meng G Y. Growth curve fitting and correlation analysis of growth traits with FOXO1 and THRSP genes in Ashidan yak[D]. Lanzhou: Chinese Academy of Agricultural Sciences, 2023.
[26]	黄兴, 柴志欣, 王会, 姬秋梅, 信金伟, 钟金城. 西藏牦牛MyoD1基因多态性及与生长性状的关联性分析[J]. 西南农业学报, 2019, 32(2):439-445.doi:10.16213/j.cnki.scjas.2019.2.035.
	Huang X, Chai Z X, Wang H, Ji Q M, Xin J W, Zhong J C. Association analysis between polymorphism of MyoD1 gene and growth traits in Tibetan yak[J]. Southwest China Journal of Agricultural Sciences, 2019, 32(2):439-445.
[27]	Ghafouri F, Bahrami A, Sadeghi M, Miraei-Ashtiani S R, Bakherad M, Barkema H W, Larose S. Omics multi-layers networks provide novel mechanistic and functional insights into fat storage and lipid metabolism in poultry[J]. Frontiers in Genetics, 2021, 12:646297.doi:10.3389/fgene.2021.646297.
[28]	Orozco G, Hinks A, Eyre S, Ke X Y, Gibbons L J, Bowes J, Flynn E, Martin P, Wilson A G, Bax D E, Morgan A W, Emery P, Steer S, Hocking L, Reid D M, Wordsworth P, Harrison P, Thomson W, Barton A, Worthington J. Combined effects of three independent SNPs greatly increase the risk estimate for RA at 6q23[J]. Human Molecular Genetics, 2009, 18(14):2693-2699.doi:10.1093/hmg/ddp193. pmid: 19417005
[29]	Zhong J L, Xu J W, Wang J, Wen Y F, Niu H, Zheng L, He H, Peng K, He P, Shi S Y, Huang Y Q, Lei C Z, Dang R H, Lan X Y, Qi X L, Chen H, Huang Y Z. A novel SNP of PLAG1 gene and its association with growth traits in Chinese cattle[J]. Gene, 2019, 689:166-171.doi:10.1016/j.gene.2018.12.018. URL

位点 Locus	引物序列(5'-3') Primer sequences (5'-3')	产物长度/bp Product length	退火温度/℃ Annealing temperature
SNP g.47,411T>C	F: GGTAATAGGTGTCAGTGAGATG	501	58.7
	R: GGAGGTTCTGGCTTCTTCT
SNP g.81,108T>C	F: TGCTTACCTGCCTTATCTGTTC	539	54.0
	R:AAGAGGTTGATTGGAAGGAAGAG

位点 Locus	引物序列(5'-3') Primer sequences (5'-3')	产物长度/bp Product length	退火温度/℃ Annealing temperature
SNP g.47,411T>C	F: GGTAATAGGTGTCAGTGAGATG	501	58.7
	R: GGAGGTTCTGGCTTCTTCT
SNP g.81,108T>C	F: TGCTTACCTGCCTTATCTGTTC	539	54.0
	R:AAGAGGTTGATTGGAAGGAAGAG

位点 Locus	基因型频率(数量) Genotype frequency (quantity)			等位基因频率 Allele frequency		P	PIC	He	Ne
位点 Locus	TT	TC	CC	T	C	P	PIC	He	Ne
g.47,411T>C	0.021 (4)	0.090(17)	0.889(168)	0.063	0.927	P<0.01	0.11	0.12	1.13
g.81,108T>C	0.020 (4)	0.086 (17)	0.894(177)	0.063	0.937	P<0.01	0.11	0.12	1.13

位点 Locus	基因型频率(数量) Genotype frequency (quantity)			等位基因频率 Allele frequency		P	PIC	He	Ne
位点 Locus	TT	TC	CC	T	C	P	PIC	He	Ne
g.47,411T>C	0.021 (4)	0.090(17)	0.889(168)	0.063	0.927	P<0.01	0.11	0.12	1.13
g.81,108T>C	0.020 (4)	0.086 (17)	0.894(177)	0.063	0.937	P<0.01	0.11	0.12	1.13

SNPs	基因型 Genotype	体质量/cm Body weight	体高/cm Body height	体长/cm Body slanting length	胸围/cm Chest circumference
g.47,411T>C	TT	247.11±19.21a	112.50±4.04a	131.00±4.69a	167.75±3.3a
	TC	223.22±43.15ab	111.29±5.14a	122.59±6.75b	160.65±13.72ab
	CC	187.56±42.07b	108.03±9.26a	116.01±9.52b	153.50±12.99b
g.81,108T>C	TT	247.31±19.58a	112.00±4.24a	131.50±5.45a	167.50±3.11a
	TC	225.49±45.08ab	111.41±5.14a	122.71±6.75b	161.29±14.11ab
	CC	190.37±42.77b	106.42±6.14a	114.74±8.14c	155.27±13.29b

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娘亚牦牛BACE2基因多态性与生长性状的关联性分析

Correlation Analysis Between BACE2 Gene Polymorphism and Growth Traits in Niangya Yak

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娘亚牦牛BACE2基因多态性与生长性状的关联性分析

Correlation Analysis Between BACE2 Gene Polymorphism and Growth Traits in Niangya Yak

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