5个绵羊品种<i>DLK1</i>基因多态性与生长性状的关联性分析

doi:10.7668/hbnxb.20193946

摘要/Abstract

摘要：

为挖掘与绵羊生长性状相关的分子标记基因,探究DLK1多态性与绵羊生长性能之间的关联性。以240头不同品种绵羊(甘肃高山细毛羊、蒙古羊、藏绵羊和小尾寒羊各50头,滩羊40 头)为试验对象,分别测定不同品种各年龄段绵羊生长性能指标;采集绵羊血液,提取DNA,运用PCR-SSCP方法结合DNA测序技术分析不同品种绵羊DLK1基因多态性;SPSS 20.0软件分析不同基因型与各绵羊品种不同年龄段生长性能之间的相关性。结果表明,不同品种绵羊DLK1基因均具有多态性,其中共检测到2个等位基因(A和B)和3种基因型(AA、AB和BB),优势等位基因和基因型分别为B和AB;DLK1基因第5内含子30 412 bp处发生G→A单个碱基突变;G30412A突变位点与绵羊1月龄和3月龄的体质量和胸围具有显著相关性(P<0.05),且BB基因型个体表型优于AA基因型个体,突变位点与绵羊体长和体高无相关性(P>0.05)。总之,不同品种绵羊DLK1基因均存在第5内含子上G30412A突变位点,该位点显著影响绵羊1,3月龄的体质量和胸围,其可作为选择甘肃地方绵羊品种生产性状的候选基因。

关键词: 绵羊, 生长性状, DLK1基因, 多态性, PCR-SSCP

Abstract:

To explore the association between DLK1 polymorphism and growth performance in sheep. A total of 240 sheep of different breeds (Gansu alpine fine wool sheep, Mongolian sheep, Tibetan sheep, Small tailed han sheep, 50 sheep each and Tan sheep, 40 sheep) were selected as the experimental subjects. The growth performance indexes of different breeds of sheep at different ages were measured. Blood samples were collected and DNA was extracted. DLK1 gene polymorphisms were analyzed by PCR-SSCP combined with DNA sequencing. SPSS 20.0 software was used to analyze the correlation between different genotypes and growth performance of each sheep breed at different ages. The results showed that DLK1 gene was polymorphic in different breeds of sheep. Two alleles (A and B) and three genotypes (AA, AB and BB) were detected, and the dominant allele and genotype were B and AB, respectively. A single nucleotide mutation (G→A) was detected at the 30 412 bp site in intron 5 of DLK1 gene. The G30412A mutation was associated with the weight and chest circumference of sheep at 1 and 3 months old. The phenotype of BB genotype sheep was better than that of AA genotype sheep. There was no correlation between the mutation sites and the body length and body height. The results of this study showed that the G30412A mutation in intron 5 of DLK1 gene existed in different breeds of sheep, and this mutation significantly affected the body weight and chest circumference of sheep at the 1 and 3 months old, which could be used as a candidate gene for the production traits of Gansu sheep breeds.

Key words: Sheep, Growth traits, DLK1 gene, Polymorphism, PCR-SSCP

孟泉禄, 张明军, 史金平, 付玲娟, 刘婷, 张全伟, 成述儒. 5个绵羊品种DLK1基因多态性与生长性状的关联性分析[J]. 华北农学报, 2023, 38(4): 233-238. doi: 10.7668/hbnxb.20193946.

MENG Quanlu, ZHANG Mingjun, SHI Jinping, FU Lingjuan, LIU Ting, ZHANG Quanwei, CHENG Shuru. Correlation Analysis Between DLK1 Gene Polymorphism and Growth Traits in Five Sheep Breeds[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4): 233-238. doi: 10.7668/hbnxb.20193946.

http://www.hbnxb.net/CN/Y2023/V38/I4/233

图/表 7

参考文献 24

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品种 Varieties	样本数/头 Number of samples	样本来源地 Source of sample	饲养方式 Feeding way
甘肃高山细毛羊 Gansu alpine fine wool sheep	50	肃南县	放牧为主,冬春季补饲
蒙古羊 Mongolian sheep	50	甘州区平山湖乡	放牧为主,冬春季补饲
滩羊 Tan sheep	40	会宁县	舍饲养殖
藏绵羊 Tibetan sheep	50	夏河县	放牧为主,冬春季不补饲或少量补饲
小尾寒羊 Small tailed han sheep	50	甘州区	舍饲养殖

品种 Varieties	样本数/头 Number of samples	样本来源地 Source of sample	饲养方式 Feeding way
甘肃高山细毛羊 Gansu alpine fine wool sheep	50	肃南县	放牧为主,冬春季补饲
蒙古羊 Mongolian sheep	50	甘州区平山湖乡	放牧为主,冬春季补饲
滩羊 Tan sheep	40	会宁县	舍饲养殖
藏绵羊 Tibetan sheep	50	夏河县	放牧为主,冬春季不补饲或少量补饲
小尾寒羊 Small tailed han sheep	50	甘州区	舍饲养殖

群体 Population	个体数 Number	基因型频率 Genotypic frequency			基因频率 Allelic frequency			遗传多态性 Genetic polymorphism
群体 Population	个体数 Number	AA	AB	BB	A	B	Ho	He	Ne	PIC
滩羊 Tan sheep	40	0.250 0	0.400 0	0.350 0	0.383 5	0.616 5	0.632 4	0.367 6	1.947 5	0.356 7
蒙古羊 Mongolian sheep	50	0.280 0	0.420 0	0.300 0	0.412 6	0.587 4	0.585 6	0.414 4	1.972 6	0.365 2
藏绵羊 Tibetan sheep	50	0.240 0	0.480 0	0.280 0	0.405 6	0.594 4	0.554 8	0.445 2	1.985 2	0.379 8
小尾寒羊 Small tailed han sheep	50	0.240 0	0.440 0	0.320 0	0.398 5	0.601 5	0.568 7	0.431 3	1.976 9	0.369 0
甘肃高山细毛羊 Gansu alpine fine wool sheep	50	0.260 0	0.460 0	0.280 0	0.468 6	0.531 4	0.535 6	0.464 4	1.990 2	0.385 6

群体 Population	个体数 Number	基因型频率 Genotypic frequency			基因频率 Allelic frequency			遗传多态性 Genetic polymorphism
群体 Population	个体数 Number	AA	AB	BB	A	B	Ho	He	Ne	PIC
滩羊 Tan sheep	40	0.250 0	0.400 0	0.350 0	0.383 5	0.616 5	0.632 4	0.367 6	1.947 5	0.356 7
蒙古羊 Mongolian sheep	50	0.280 0	0.420 0	0.300 0	0.412 6	0.587 4	0.585 6	0.414 4	1.972 6	0.365 2
藏绵羊 Tibetan sheep	50	0.240 0	0.480 0	0.280 0	0.405 6	0.594 4	0.554 8	0.445 2	1.985 2	0.379 8
小尾寒羊 Small tailed han sheep	50	0.240 0	0.440 0	0.320 0	0.398 5	0.601 5	0.568 7	0.431 3	1.976 9	0.369 0
甘肃高山细毛羊 Gansu alpine fine wool sheep	50	0.260 0	0.460 0	0.280 0	0.468 6	0.531 4	0.535 6	0.464 4	1.990 2	0.385 6

时间 Time	指标 Indicators	基因型 Genotype			P值 P values
时间 Time	指标 Indicators	AA	AB	BB	P值 P values
1月龄	初生质量/kg	3.04±0.04ns	3.03±0.04ns	3.08±0.04ns	0.61ns
1 month old	体质量/kg	7.08±0.12ns	7.11±0.10ns	7.02±0.11ns	0.83ns
	体长/cm	42.69±0.40a	47.98±0.32ab	49.95±0.34b	0.04^*
	体高/cm	46.32±0.34ns	47.25±0.28ns	48.62±0.30ns	0.64ns
	胸围/cm	43.53±0.42a	48.97±0.35ab	52.57±0.37b	0.04^*
3月龄	体质量/kg	18.03±0.17ns	17.88±0.14ns	18.02±0.15ns	0.73ns
3 months old	体长/cm	53.33±0.32a	56.71±0.26ab	59.99±0.28b	0.03^*
	体高/cm	53.17±0.30ns	53.36±0.24ns	53.13±0.26ns	0.78ns
	胸围/cm	59.99±0.44a	64.21±0.36ab	68.71±0.39b	0.03^*
6月龄	体质量/kg	25.09±0.26ns	25.26±0.20ns	24.95±0.22ns	0.57ns
6 months old	体长/cm	60.92±0.42ns	62.21±0.33ns	65.89±0.35ns	0.68ns
	体高/cm	59.31±0.42ns	59.62±0.33ns	59.82±0.36ns	0.65ns
	胸围/cm	65.52±0.59ns	69.92±0.47ns	70.45±0.50ns	0.13ns
9月龄	体质量/kg	30.24±0.30ns	30.12±0.24ns	29.76±0.26ns	0.42ns
9 months old	体长/cm	67.19±0.61ns	67.21±0.49ns	70.44±0.53ns	0.93ns
	体高/cm	64.37±0.71ns	64.13±0.57ns	64.54±0.61ns	0.88ns
	胸围/cm	72.61±0.79ns	75.05±0.64ns	78.02±0.68ns	0.28ns

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5个绵羊品种DLK1基因多态性与生长性状的关联性分析

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5个绵羊品种DLK1基因多态性与生长性状的关联性分析

Correlation Analysis Between DLK1 Gene Polymorphism and Growth Traits in Five Sheep Breeds

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