玉米花丝花青苷显色性状的QTL定位

doi:10.7668/hbnxb.20194658

摘要/Abstract

摘要：

玉米花丝颜色是玉米特异性和一致性判定的重要农艺性状。为解析玉米花丝花青苷显色性状的遗传机制,以绿色花丝自交系WL134和紫色花丝自交系D7杂交构建的213份DH系群体为研究材料,在2022年和2023年2 a环境下分别进行QTL定位分析。结果表明,花丝花青苷显色性状在不同家系、不同年份之间均存在显著差异,且广义遗传力为0.864。2 a的花丝颜色数据共检测到9个QTLs,分布于玉米的第2,3,5,6,8,9号染色体上,单个QTL的表型贡献率在4.83%~9.26%。在第5染色体上定位到一个稳定的在2 a数据中可重复检测到的位点qSC5,位于19.15~19.80 Mb,2022年检测的LOD值为4.65,贡献率7.22%,2023年检测的LOD值为5.76,贡献率7.17%,为主效QTL位点。与前人的研究比对发现,该位点为调控花丝花青苷显色的新位点。基于qSC5两侧的SNP标记,DH群体中极端紫色花丝家系中90.91%的基因型为CCCC,而绿色花丝家系中该基因型仅占44.00%。该标记与玉米花丝颜色显著相关,可能与调控花丝花青苷显色性状的关键基因连锁。

关键词: 玉米, 花丝颜色, QTL定位, SNP标记

Abstract:

Silk color is an important agronomic trait for determining distinctiveness and uniformity of maize variety.In order to analyze the genetic mechanism of silk coloring characteristics of anthocyanins in maize,a doubled haploid(DH)population with 213 lines derived from green silk inbred line WL134 and purple silk inbred line D7 was used for QTL mapping analysis under the environment of 2022 and 2023,respectively.The results showed that there were significant differences in silk coloring characteristics of anthocyanins among different lines and years,the heritability was 0.864.A total of 9 QTLs were detected in two years.These QTLs with phenotypic variation explained(PVE)ranging from 4.83% to 9.26% were detected on chromosomes 2,3,5,6,8,and 9 of maize.A stable and repeatable site qSC5 located between 19.15 Mb and 19.80 Mb was detected on chromosome 5 in two-year data.The LOD scores of the major QTLs were 4.65 and 5.76 in 2022 and 2023 respectively,with phenotypic variation explained(PVE)of 7.22% and 7.17%.It was a new site for regulating silk coloring characteristics of anthocyanins compared with previous studies in maize.Based on SNP markers on both sides of qSC5,90.91% of the genotypes in extreme purple silk DH lines were CCCC,while only 44.00% of the genotypes in green silk DH lines.This marker was significantly correlated with silk color in maize and might link to key genes regulating silk color characteristics of anthocyanins.

Key words: Maize, Silk color, QTL mapping, SNP marker

黄缓缓, 安洪周, 李魁英, 王延兵, 谷亦, 乔亚科, 高增玉. 玉米花丝花青苷显色性状的QTL定位[J]. 华北农学报, 2024, 39(4): 31-36. doi: 10.7668/hbnxb.20194658.

HUANG Huanhuan, AN Hongzhou, LI Kuiying, WANG Yanbing, GU Yi, QIAO Yake, GAO Zengyu. QTL Mapping of Silk Coloring Characteristics of Anthocyanins in Maize[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 31-36. doi: 10.7668/hbnxb.20194658.

http://www.hbnxb.net/CN/Y2024/V39/I4/31

图/表 7

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差异源 Source of variance	平方和 SS	自由度 df	均方 MS	F值 F-value	P值 P-value	显著性 Significance	遗传力 H²
基因型(G)	623.600	212	2.942	10.426	3.53E-90	^**
年份(Y)	29.719	1	29.719	105.333	3.36E-22	^**
基因型×年份(G×Y)	73.043	208	0.351	1.245	0.032	^*
误差(E)	118.500	420	0.282
总计(T)	847.122	841					0.864

差异源 Source of variance	平方和 SS	自由度 df	均方 MS	F值 F-value	P值 P-value	显著性 Significance	遗传力 H²
基因型(G)	623.600	212	2.942	10.426	3.53E-90	^**
年份(Y)	29.719	1	29.719	105.333	3.36E-22	^**
基因型×年份(G×Y)	73.043	208	0.351	1.245	0.032	^*
误差(E)	118.500	420	0.282
总计(T)	847.122	841					0.864

年份 Year	QTL名称 QTL name	染色体 Chr	位置/Mb Pos	标记区间(左)/bp Left marker	标记区间(右)/bp Right marker	LOD值 LOD	贡献率/% PVE	加性效应 Add
2022	qSC2-1	2	0	chr2-8786937	chr2-8786986	5.88	9.26	0.258 2
2022	qSC5	5	58	chr5-19156257	chr5-19800985	4.65	7.22	0.199 3
2022	qSC6-1	6	81	chr6-141474871	chr6-142274037	3.39	5.35	0.173 2
2022	qSC8	8	86	chr8-140614324	chr8-142202388	4.23	6.63	0.190 4
2022	qSC9	9	108	chr9-142103909	chr9-142308333	4.05	6.31	-0.185 7
2023	qSC2-2	2	23	chr2-8955652	chr2-9007579	4.54	5.59	0.214 7
2023	qSC2-3	2	182	chr2-231587850	chr2-231194135	4.78	5.89	0.221 5
2023	qSC3	3	150	chr3-218266161	chr3-218273050	4.46	5.60	-0.213 0
2023	qSC5	5	58	chr5-19156257	chr5-19800985	5.76	7.17	0.244 4
2023	qSC6-2	6	95	chr6-149567395	chr6-150880011	3.96	4.83	0.204 3

年份 Year	QTL名称 QTL name	染色体 Chr	位置/Mb Pos	标记区间(左)/bp Left marker	标记区间(右)/bp Right marker	LOD值 LOD	贡献率/% PVE	加性效应 Add
2022	qSC2-1	2	0	chr2-8786937	chr2-8786986	5.88	9.26	0.258 2
2022	qSC5	5	58	chr5-19156257	chr5-19800985	4.65	7.22	0.199 3
2022	qSC6-1	6	81	chr6-141474871	chr6-142274037	3.39	5.35	0.173 2
2022	qSC8	8	86	chr8-140614324	chr8-142202388	4.23	6.63	0.190 4
2022	qSC9	9	108	chr9-142103909	chr9-142308333	4.05	6.31	-0.185 7
2023	qSC2-2	2	23	chr2-8955652	chr2-9007579	4.54	5.59	0.214 7
2023	qSC2-3	2	182	chr2-231587850	chr2-231194135	4.78	5.89	0.221 5
2023	qSC3	3	150	chr3-218266161	chr3-218273050	4.46	5.60	-0.213 0
2023	qSC5	5	58	chr5-19156257	chr5-19800985	5.76	7.17	0.244 4
2023	qSC6-2	6	95	chr6-149567395	chr6-150880011	3.96	4.83	0.204 3

极端紫花丝家系 Extreme purple silk lines	基因型 Genotype	花丝颜色分级 Silk color grading		极端绿花丝家系 Extreme green silk lines	基因型 Genotype	花丝颜色分级 Silk color grading
极端紫花丝家系 Extreme purple silk lines	基因型 Genotype	2022	2023	极端绿花丝家系 Extreme green silk lines	基因型 Genotype	2022	2023
L46	CCCC	4	6	L20	TTAA	1	1
L116	CCCC	5	4	L34	TTAA	1	1
L194	CCCC	4	4	L95	TTAA	1	1

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