摘要：

密刺蔷薇是现代月季育种工作中宝贵的种质资源,然而其具有较高休眠特性,种子发芽困难,无法进行大规模种子繁殖。通过研究低温沙藏方式下密刺蔷薇种子内含物变化,探究影响其种子萌发的因素,为密刺蔷薇进一步推广应用提供理论基础。通过ELISA法和微量法分别测定材料的激素含量、有机成分和酶含量及活性,并通过单因素分析方差和显著性检验对其内含物进行分析比较。密刺蔷薇沙藏前后种子激素含量差异不显著;低温沙藏处理降低了密刺蔷薇种子可溶性蛋白、淀粉和可溶性糖等有机成分含量;沙藏处理对密刺蔷薇种子的抗氧化酶活性有较大影响,在低温沙藏并播种10个月后其抗氧化酶活性显著提高。低温处理不是限制密刺蔷薇种子萌发的主要原因,反而有利于解除密刺蔷薇种子的休眠。研究为进一步大规模生产繁殖新疆野生蔷薇属资源提供了一定的理论基础及实践指导经验。

关键词: 新疆野生蔷薇, 低温沙藏, 激素, 有机成分, 抗氧化酶, 种子萌发

Abstract:

Rosa spinosissima is valuable germplasm resource for modern rose breeding.R.spinosissima with a wide distribution range has high dormancy characteristics,seed germination is difficult and seed propagation cannot be carried out.It studied the changes of seed contents under different storage methods to explore the factors affecting seed germination.The contents of hormones,organic components and enzymes were respectively determined by ELISA and microassay.The contents were analyzed and compared by one-way analysis of variance and significance test.The results showed that there was no significant difference in the content of seed hormone before and after the accumulation of R.spinosissima.Low temperature sand storage treatment could change the content of organic components and reduce the content of soluble protein,starch and soluble sugar in R.spinosissima seeds.The antioxidant enzyme activities of R.spinosissima seeds were greatly affected by sand storage treatment,and the antioxidant enzyme activities of R.spinosissima seeds were significantly increased after 10 months of low temperature sand storage and sowing.Low temperature treatment was not the main reason to limit the germination of R.spinosissima seeds,but was beneficial to relieve the dormancy of R.spinosissima seeds.It provided some theoretical basis and practical experience for further large-scale production and propagation of wild Rosa resources in Xinjiang.

Key words: Rosa from Xinjiang, Low temperature sand storage, Hormone, Organic composition, Antioxidant enzyme, Seed germination