水稻DUF760基因家族的全基因组鉴定与表达谱分析

doi:10.7668/hbnxb.20193226

摘要/Abstract

摘要：

为了探索DUF760基因家族在水稻生长发育中的潜在功能,对水稻DUF760基因家族进行了全基因组鉴定、分类、启动子序列分析和表达谱分析。通过生物信息学技术在水稻和拟南芥中分别鉴定了6,8个DUF760家族成员。系统进化树分析将这些家族成员分成2个亚家族,二者在蛋白保守基序和基因结构上也存在一些特征差别。水稻DUF760家族基因启动子区域存在多个响应逆境胁迫和植物激素的顺式作用元件,ABRE(脱落酸响应)元件存在于家族所有成员的启动子序列中,OsDUF760-1启动子区域拥有9个脱落酸(ABA)相关的响应元件。在ABA处理水稻后,OsDUF760-1的转录表达水平显著下调,而OsDUF760-3显著上调,二者在干旱胁迫处理水稻后的表达变化模式与ABA处理水稻后的表达变化模式一致,说明这2个基因可能通过ABA信号途径参与水稻干旱胁迫响应,并且扮演不同的角色。除对ABA和干旱胁迫处理具有较强响应外,水稻DUF760家族成员还对JA(茉莉素)、低温及稻瘟菌处理具有较强的响应表达变化。

关键词: 水稻, DUF760基因家族, 逆境, 植物激素, 表达谱分析

Abstract:

To investigate the potential functions of DUF760s in rice growth and development,the genome-wide identification,classification,promoter sequence and expression profile analysis of the DUF760 gene family were performed.This research identified 6 and 8 members of DUF760 gene family in rice and Arabidopsis respectively by bioinformatics analysis.Phylogenetic tree analysis divided these members into two subfamilies,there were also some characteristic differences between the two subfamilies in protein conserved motif and gene structure.Multiple cis-acting elements responding to stresses and phytohormones existed in the promoter regions of rice DUF760 family genes.ABRE(abscisic acid response)element was present in the promoter sequence of all DUF760 family members,the promoter region of OsDUF760-1 possessed 9 abscisic acid(ABA)related response elements.The transcriptional expression level of OsDUF760-1 in rice was significantly down-regulated after ABA treatment,while OsDUF760-3 was significantly up-regulated.The expression change patterns of these two genes in rice after drought stress treatment were consistent with that after ABA treatment,which indicated that these two genes may participate in rice drought stress response through ABA signaling pathway,and play different roles.In addition to strong responses to ABA and drought stress treatments,members of rice DUF760 family also showed relatively strong expression changes in response to JA(Jasmonic acid),low temperature and M.oryzae treatments.

Key words: Rice, DUF760 gene family, Stress, Phytohormone, Expression profile analysis

杜强, 韩玲玲, 肖秀文, 李锦程, 沈梦宇, 王志龙, 陈秋红. 水稻DUF760基因家族的全基因组鉴定与表达谱分析[J]. 华北农学报, 2023, 38(1): 1-8. doi: 10.7668/hbnxb.20193226.

DU Qiang, HAN Lingling, XIAO Xiuwen, LI Jincheng, SHEN Mengyu, WANG Zhilong, CHEN Qiuhong. Genome-Wide Identification and Expression Profile Analysis of DUF760 Gene Family in Rice[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 1-8. doi: 10.7668/hbnxb.20193226.

http://www.hbnxb.net/CN/Y2023/V38/I1/1

图/表 9

表1 OsDUF760家族成员相对表达量检测所用引物

Tab.1 Primers used for relative expression level detection of OsDUF760 family members

基因名称 Gene name	引物序列(5'—3') Primer sequence (5'—3')
OsDUF760-1	F: CCGGCCAAGATAACTGTATTTG
	R: CCAACAATAGTCAGGTTGCAAA
OsDUF760-2	F: CTACATCCGAGGTCTTGAGTAC
	R: TCTTTCTGAGGAGAAATGCAGT
OsDUF760-3	F: AAACGAATTTCTCCTGGCTCTA
	R: GAACTAACAGCTGCACATTAGG
OsDUF760-4	F: GCATGTATTGTTAGTCGCACAA
	R: AAATGCATCCTCCACTAGAACA
OsDUF760-5	F: CTGATTCTTGCTCAGTCACAAG
	R: GGATCAAAGCCCATCATGTAAC
OsDUF760-6	F: CAGCCCCTGATATCTATTCCTC
	R: GGAGATCATGAATGTGCATCAC
Ubiquitin	F: AACCAGCTGAGGCCCAAGA
	R: ACGATTGATTTAACCAGTCCATGA

表2 水稻和拟南芥DUF760家族成员信息

Tab.2 Information of DUF760 family members in rice and arabidopsis

基因名称 Gene name	基因序列号 Gene locus ID	编码序列长度/bp CDS length	氨基酸长度/aa Amino acid length	等电点 pI	分子量/ku MW
OsDUF760-1	Os02g0833400	1 011	336	6.58	36.931
OsDUF760-2	Os03g0607500	1 218	405	4.94	44.787
OsDUF760-3	Os03g0717900	1 368	455	9.24	49.956
OsDUF760-4	Os04g0596300	1 218	405	5.51	45.306
OsDUF760-5	Os11g0456100	1 167	388	8.28	44.084
OsDUF760-6	Os12g0581700	1 182	393	5.10	43.185
AtDUF760-1	At1g32160	1 221	406	5.54	45.868
AtDUF760-2	At1g48450	1 272	423	5.86	47.189
AtDUF760-3	At1g63610	1 023	340	6.26	37.955
AtDUF760-4	At2g14910	1 161	386	5.04	43.396
AtDUF760-5	At3g07310	1 107	368	8.46	41.368
AtDUF760-6	At3g17800	1 266	421	9.20	46.067
AtDUF760-7	At5g14970	1 068	355	5.57	39.570
AtDUF760-8	At5g48590	1 035	344	7.67	38.959

图1 DUF760家族成员的系统进化树和保守基序分析

Fig.1 Phylogenetic tree and motif analysis of DUF760 family members

图2 DUF760家族成员的基因结构分析

Fig.2 Gene structure analysis of DUF760 family members

图3 OsDUF760家族基因启动子中的顺式作用元件鉴定

Fig.3 Cis-acting elements identification in promoters of OsDUF760 family genes

图4 OsDUF760家族基因的组织表达谱分析

Fig.4 Tissue expression profile analysis of OsDUF760 family genes

图5 OsDUF760家族基因的激素响应表达谱分析不同小写字母表示有显著差异(P<0.05)。图6,7同。

Fig.5 Expression profile analysis of OsDUF760 family genes in response to hormone treatments Different lowercase letters showed significantly difference(P<0.05).The same as Fig.6,7.

图6 OsDUF760家族基因的胁迫响应表达谱分析

Fig.6 Expression profile analysis of OsDUF760 family genes in response to abiotic stresses

图7 OsDUF760家族基因的生物胁迫响应表达谱分析

Fig.7 Expression profile analysis of OsDUF760 family genes in response to biotic stress

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