转录组及代谢组联合解析玉米响应花粒期高温胁迫机制

doi:10.7668/hbnxb.20190649

摘要/Abstract

摘要： 为了探明我国玉米主要推广品种郑单958、先玉335花粒期高温胁迫下基因表达及代谢物差异，挖掘玉米响应高温胁迫的关键基因及代谢物，利用Illumina HiSeq^TM 2500高通量测序技术分别对郑单958、先玉335高温胁迫7，14 d的穗位叶及对照叶进行高通量转录组测序，利用广泛靶向代谢组测序技术对样品进行高通量代谢物测序。转录组测序共获得214.81 Gb干净序列。郑单958高温胁迫7 d后检测到49个差异表达基因，其中24个为上调基因。继续高温胁迫14 d共检测到306个差异表达基因，其中130个为上调基因。高温胁迫7 d与高温胁迫14 d对比组中检测到1 462个差异表达基因，其中647个为上调基因。先玉335高温胁迫7 d后共检测到381个差异表达基因，164个上调基因。继续高温胁迫14 d后共检测到299个差异表达基因，226个为上调基因。高温胁迫7 d与高温胁迫14 d对比组中共检测到2 481个差异表达基因，其中1 275个上调基因。在郑单958、先玉335高温胁迫7 d后对比组中检测到6 646个差异表达基因，其中3 253个上调基因。在郑单958、先玉335高温胁迫14 d对比组中检测到5 958个差异表达基因，其中3 110个上调基因。代谢物测序共检测到654个代谢物，郑单958高温胁迫7 d后共检测出28个差异代谢物，共注释到5个代谢通路中。高温胁迫14 d后共检测出54个差异代谢物，共注释到13个代谢通路中，其中9个差异代谢物呈上调表达。先玉335高温胁迫7 d后检测出98个差异代谢物，共注释到24个代谢通路中，其中43个差异代谢物呈上调表达。先玉335高温胁迫14 d后共检测出38个差异代谢物，共注释到13个代谢通路中，其中14个上调表达。郑单958高温胁迫7 d与先玉335高温胁迫7 d对比组共检测出144个差异代谢物，共注释到36个代谢通路中，其中81个差异代谢物呈上调表达。郑单958高温胁迫14 d与先玉335高温胁迫14 d对比组共检测出158个差异代谢物，共注释到40个代谢通路中，其中81个差异代谢物呈上调表达。

关键词: 玉米, 高温胁迫, 花粒期, 高通量转录组测序, 代谢组学分析, 差异表达基因

Abstract: The aim of the study was to explore differential expression of genes and metabolites in Zhengdan 958 and Xianyu 335 exposed to high temperature for 7,14 d during grain filling stage and identify the key candidate genes,transcription factors and metabolites,so as to understand molecular mechanism of high temperature tolerance in maize. The ear-leaf samples collected before high temperature treated(CK),7 d high temperature treated,14 d from Zhengdan 958 and Xianyu 335 were used for RNA-sequencing using Illumina HiSeq^TM 2500 high-throughput sequencing technology. Metabolome variations analyzed using liquid-chromatography-mass spectrometry-based metabolomics. A total of 214.81 Gb clean sequence was obtained by transcriptome sequencing. In Zhengdan 958,49 DEGs were detected after 7 days of high temperature treated,of which 24 were up-regulated genes. 306 DEGs were detected after 14 d,130 of which were up-regulated genes. 1 462 DEGs were detected from 7 d samples compared with 14 d samples,of which 647 were up-regulated genes. In Xianyu 335,381 DEGs were detected after 7 days of high temperature treated,of which 164 were up-regulated genes. 299 DEGs were detected after 14 d,226 of which were up-regulated genes. 2 481 DEGs were detected from 7 d treated samples compared with 14 d treated samples,of which 1 275 were up-regulated genes. Comparing Zhengdan 958 samples treated for 7 d with Xianyu 335 samples treated for 7 d,6 646 DEGs were detected,of which 3 253 up-regulated genes. Comparing Zhengdan 958 samples treated for 14 d with Xianyu 335 samples treated for 14 d,5 958 DEGs were detected,of which 3 110 up-regulated genes. A total of 654 metabolites were detected by metabolomics sequencing. In Zhengdan 958,28 DEMs were detected after 7 days of high temperature treated and were annotated into 5 metabolic pathways. 54 DEMs were detected after 14 days,9 of which were up-regulated,and were annotated into 13 metabolic pathways. In Xianyu 335, 98 DEMs were detected after 7 d of high temperature treated,43 of which were up-regulated and were annotated into 24 metabolic pathways. 38 DEMs were detected after 14 d,14 of which were up-regulated,and were annotated into 13 metabolic pathways. Compared Zhengdan 958 with Xianyu 335,144 DEMs were detected after 7 days,81 of which were up-regulated,and were annotated into 36 metabolic pathways.158 DEMs were detected after 14 days,81 of which were up-regulated,and were annotated into 40 metabolic pathways.

Key words: Maize, High temperature stress, Anthesis stage, Illumina RNA-sequencing, Metabolomics profiling, Differentially expressed gene(DEG)

中图分类号:

S513

李川, 乔江方, 黄璐, 张美微, 张盼盼, 牛军, 刘京宝. 转录组及代谢组联合解析玉米响应花粒期高温胁迫机制[J]. 华北农学报, 2020, 35(1): 8-21. doi: 10.7668/hbnxb.20190649.

LI Chuan, QIAO Jiangfang, HUANG Lu, ZHANG Meiwei, ZHANG Panpan, NIU Jun, LIU Jingbao. Transcriptome and Metabolome Analysis to Reveal the Mechanisms Responding to High Temperature Stress in Anthesis Stage of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 8-21. doi: 10.7668/hbnxb.20190649.

http://www.hbnxb.net/CN/Y2020/V35/I1/8

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