摘要: 为了研究球孢白僵菌HFW-05菌株在土壤中的宿存情况,采用土壤拌菌法进行了初步探索.以初始土壤拌菌量1.0×107个孢子/mL浓度,连续观察60 d,结果表明,球孢白僵菌在土壤中宿存2个月内,成菌落数呈波动下降趋势,下降速度较慢,在释放的30~60 d,白僵菌处于平稳波动状态,成菌落数持续在106cfu/g,同时球孢白僵菌释放前后,对小菜蛾的毒力无显著差异,对土壤微生物种群数量亦无明显影响,不会破坏原有的生态平衡.
关键词:
球孢白僵菌,
土壤,
宿存
Abstract: In order to research the Beauveria bassiana HFW-05 strain in soil in persistent storage,the soil mix bacteria method had carried on the preliminary exploration.Initial bacterial biomass was 1.0×107spore/mL concentrations.Continuous observation 60 d,the results showed that Beauveria bassiana in soil persistent within two months,a number of colonies showed a downward trend involatility,slower rate of decline,in the release of 30-60 d, Beauveria bassiana in a stable state of fluctuation,CFU continued in the 106 cfu/g.At the same time,the number of soil microorganism population had little effect.There was no significant difference before and after the release,the virulence of Beauveria bassiana against Plutella xylostella,didn't destroy the original ecological balance.
Key words:
Beauveria bassiana,
Soil,
Persistent
中图分类号:
宋健, 曹伟平, 张海剑, 王广君, 杜立新. 球孢白僵菌HFW-05菌株在土壤中宿存研究初探[J]. 华北农学报, 2015, 30(S1): 333-335. doi: 10.7668/hbnxb.2015.S1.060.
SONG Jian, CAO Wei-ping, ZHANG Hai-jian, WANG Guang-jun, DU Li-xin. The Preliminary Study of Beauveria bassiana Survived in Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 333-335. doi: 10.7668/hbnxb.2015.S1.060.