华北农学报 ›› 2015, Vol. 30 ›› Issue (6): 1-7. doi: 10.7668/hbnxb.2015.06.001

所属专题: 水稻

• 论文 • 上一篇    下一篇

水稻苗期耐低氮相关性状的QTL定位

赵春芳, 赵凌, 张亚东, 陈涛, 赵庆勇, 朱镇, 周丽慧, 姚姝, 王才林   

  1. 江苏省农业科学院 粮食作物研究所, 江苏省优质水稻工程技术研究中心, 国家水稻改良中心南京分中心, 江苏 南京 210014
  • 收稿日期:2015-09-26 出版日期:2015-12-28
  • 通讯作者: 王才林(1959-),男,江苏无锡人,研究员,博士,博士生导师,主要从事水稻遗传育种研究.
  • 作者简介:赵春芳(1981-),女,山东聊城人,副研究员,博士,主要从事水稻遗传育种研究.
  • 基金资助:
    国家自然科学基金项目(31200144);现代农业产业技术体系建设专项基金项目(CARS-01-47);江苏省科技支撑计划项目(BE2013301)

QTL Mapping for Seedling Traits Related to Low Nitrogen Tolerance in Rice

ZHAO Chun-fang, ZHAO Ling, ZHANG Ya-dong, CHEN Tao, ZHAO Qing-yong, ZHU Zhen, ZHOU Li-hui, YAO Shu, WANG Cai-lin   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2015-09-26 Published:2015-12-28

摘要: 水稻低氮相关性状的遗传分析对研究氮素吸收和利用效率及培养耐性品种具有重要意义.缺氮是限制作物生长和产量提高的重要因素.利用一套以9311为遗传背景携带日本晴置换片段的染色体片段置换系为材料,对低氮胁迫下水稻苗期性状进行了QTL定位.共定位到高氮和低氮下株高、主根长、根干质量、苗干质量和总干质量及其相对性状的44个QTLs,其中2种氮水平下检测到28个QTLs,低氮与高氮相对性状检测到16个QTLs.检测到2个QTL热点区,聚集了不同氮水平及其相对性状的多个QTLs,通过搜索预测基因,发现这2个区域包含氮代谢的关键基因,说明极有可能是氮吸收和利用相关基因控制了这些QTLs的表达.以上结果将为利用分子标记辅助选育耐低氮品种提供依据.

关键词: 水稻, 低氮胁迫, 根长, 根干质量, QTL

Abstract: Genetic analysis of low nitrogen related traits has important significance for the study of nitrogen uptake and use efficiency and the breeding of novel varieties with low nitrogen tolerance in rice.In this study,a set of chromosome segment substitution lines (CSSLs) developed from two sequenced rice cultivars:the indica cultivar 9311 as recipient and the japonica cultivar Nipponbare as donor were used to detect for QTLs of rice seedling traits under low nitrogen stress.44 QTLs including 28 QTLs under two N levels and 16 QTLs of their relative traits were mapped.Two QTL hotspots gathered with multiple QTLs under different N levels and the relative traits were found.By searching the predicted genes,the two regions were found to contain several N metabolism genes.Therefore,genes related to N absorption and utilization probably control the expression of these QTLs.This result will be useful in breeding rice varieties with low N tolerance by molecular assisted marker.

Key words: Oryza sativa L., Low nitrogen stress, Root elongation, Root dry weight, QTL

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引用本文

赵春芳, 赵凌, 张亚东, 陈涛, 赵庆勇, 朱镇, 周丽慧, 姚姝, 王才林. 水稻苗期耐低氮相关性状的QTL定位[J]. 华北农学报, 2015, 30(6): 1-7. doi: 10.7668/hbnxb.2015.06.001.

ZHAO Chun-fang, ZHAO Ling, ZHANG Ya-dong, CHEN Tao, ZHAO Qing-yong, ZHU Zhen, ZHOU Li-hui, YAO Shu, WANG Cai-lin. QTL Mapping for Seedling Traits Related to Low Nitrogen Tolerance in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 1-7. doi: 10.7668/hbnxb.2015.06.001.

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