摘要： 为了解新疆阿勒泰地区的捕食线虫性真菌分布情况,采用玉米粉琼脂培养基(CMA)培养分离阿勒泰地区土壤中的捕食线虫性真菌,通过对纯化后菌株的形态学观察和分子生物学分析,鉴定出5株少孢节丛孢属真菌和1株圆锥节丛孢菌。另外用质量浓度分别为0.5,0.05,0.005 g/L的L-苯丙氨酸、L-色氨酸、L-蛋氨酸和L-缬氨酸对已分离的圆锥节丛孢菌AS3株进行捕食器生成的诱导反应,发现各组氨基酸对捕食器的产生均有诱导效应,但质量浓度为0.05 g/L的L-苯丙氨酸诱导产生的捕食器总数最多。为利用捕食线虫性真菌进行生物防控奠定了理论基础。

关键词: 捕食线虫性真菌, 分离鉴定, 氨基酸, 捕食器官形成

Abstract: Nematode-trapping fungi were isolated with corn meal agar(CMA) from soils samples of Altay region in Xinjiang Province. 6 strains were successfully isolated and five strains identified as Arthrobotrys oligospora and the other one is Arthrobotrys conoides through morphological characteristics and molecular biology analysis. In addition, four amino acids(L-phenylalanine,L-tryptophan,L-methionine and L-valine) in different concentrations(0. 5, 0. 05,0. 005 g / L) were used as revulsive to induce the nematode-trapping organs forming of AS3 strain. The results showed that all the amino acids had effects on the traps forming,but the L-phenylalanine in concentration of 0. 05 g / L induced maximum number of trapping devices. This study laid a good foundation for biological prevention and control by nematode-trapping fungi.

Key words: Nematode-trapping fungus, Isolation and identification, Amino acids, Nematode-trapping devices forming

中图分类号: 

• S432.4