摘要： 花粉生活力是评估花粉细胞活性的依据之一,其活力测定结果的准确性决定细胞学试验和杂交育种的成败。本研究以32个百合品种的花粉为试验材料,采用染色法和花粉离体萌发法,探讨了不同百合品种新鲜花粉生活力、萌发率的差异,不同贮藏条件下同品种花粉生活力的差异。结果表明:离体萌发法的测定结果更能反映花粉活力,CasaBlanca的萌发率最高,为87.33%,Yelloween的萌发率最低,为1.33%。对于相同的贮藏条件即室温贮藏来说,因品种和贮藏时间的不同花粉生活力变化有明显差异,总的趋势为随贮藏时间延长,萌发率下降,但不同基因型间下降速度不同。在冷藏条件下,花粉萌发率随贮藏时间的推移总体也呈下降趋势,但下降速度比室温贮藏的明显缓慢,且不同品种的变化亦有所差别。

关键词: 花粉, 贮藏条件, 生活力, 萌发率

Abstract: Pollen viability is to assess the activity of one of the basis of pollen cells ,The activity assay results of cytological experiments and determine the accuracy of breeding success or failure. In this study , 32 lily cultivars as experimental materials,comparison of different varieties of fresh lily pollen viability, germination rate differences and different storage conditions hereinafter pollen viability differences by staining method and in vitro pollen germination method. The results showed that:It is more accurate to pollen viability of in vitro pollen germination method.Germination rate of Casa Blanca is highest namely 87. 33%,Yelloween is lowest namely 1.33 %.To uniform storage dry condition in room, pollen viability of different lily cultivars is obvious different by different storage time. Germination rate decrease as extension as storage times, but rate of descend is different by different cultivars. Pollen germination rate decrease as extension as storage times in cold storage,but rate of descend is slower than in room storage.

Key words: Pollen, Storage conditions, Vitality, Germination rate

中图分类号: 

• S682.2