茉莉花粉离体培养萌发及花粉管生长特性研究

doi:10.7668/hbnxb.2014.05.018

摘要/Abstract

摘要： 为了揭示茉莉花粉育性并为其活力检测提供一种高效方法,研究了取粉时间、基本培养基种类及不同添加物浓度、pH值、温度、光照、时间等因素对花粉离体培养萌发和花粉管生长的影响,并应用扫描电子显微镜观察了花粉在柱头上的萌发情况,以对检测方法进行验证。结果显示,早上8:00-9:00所取花粉活力最高,适宜萌发的培养基为BK(成分为100 mg/L的H₃BO₃、100 mg/L的KNO₃、200 mg/L的MgSO₄·7H₂O和300 mg/L的Ca(NO₃)₂·4H₂O)+蔗糖80 g/L+PEG4000 120 g/L,适宜的pH值6.0,最佳培养温度为25~30 ℃、光照为35~50 μmol/(m²·s)、时间为3~4 h。相关性分析发现,花粉萌发和花粉管生长均与光照强度和培养时间呈极显著正相关(P<0.01),与培养基pH值呈正相关、与蔗糖和PEG4000浓度均呈负相关、与培养温度分别呈负相关和正相关,但相关性均未达显著水平(P>0.05)。研究表明,茉莉花粉总体上活力较低,离体培养萌发不失为一种简单、快速、可靠的检测方法,但应注意对培养条件的选择和优化以达到最佳效果。

关键词: 茉莉, 花粉活力, 花粉培养, 花粉管生长, 扫描电子显微镜

Abstract: To reveal pollen viability in jasmine and provide an efficient method for the detecting,the effects of sampling stage,medium type,concentrations of different additions,pH value,culture temperature,light intensity and time on the pollen germination and pollen tube growth were investigated.Meanwhile,the method was identified further through observing the pollen germinated on stigmas under scanning electron microscopy.The results showed that pollen of the highest viability should be collected at 8:00-9:00 am,the optimal medium was BK(Containing H₃BO₃ 100 mg/L,KNO₃ 100 mg/L,MgSO₄·7H₂O 200 mg/L and Ca(NO₃)₂·4H₂O 300 mg/L)added with sucrose 80 g/L and PEG4000 120 g/L,with the suitable pH value of 6.0,temperature of 25-30 ℃,light intensity of 35-50 μmol/(m²·s)and culture time of 3-4 h.Correlation analysis showed that pollen germination and pollen tube growth were very significantly positively correlated with the indices of light intensity and culture time(P<0.01),positively with the pH value,negatively with the concentrations of sugar and PEG4000,and negatively and positively with the temperature,respectively,at no significant levels(P>0.05).The research shows that jasmine pollen has a low viability on the whole,and germination in vivo was indeed a simple,quick and reliable method to determine the viability.But much attention should be paid to the selection and optimization of culture conditions to obtain the best effectiveness.

Key words: Jasmine, Pollen viability, Pollen culture, Pollen tube growth, Scanning electron microscopy

中图分类号:

S685.03

邓衍明, 叶晓青, 梁丽建, 贾新平. 茉莉花粉离体培养萌发及花粉管生长特性研究[J]. 华北农学报, 2014, 29(5): 107-113. doi: 10.7668/hbnxb.2014.05.018.

DENG Yan-ming, YE Xiao-qing, LIANG Li-jian, JIA Xin-ping. Pollen Germination in vitro and Pollen Tube Growth of Jasminum sambac Aiton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 107-113. doi: 10.7668/hbnxb.2014.05.018.

http://www.hbnxb.net/CN/Y2014/V29/I5/107

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