摘要： 利用黑麦基因组特异SCAR标记对小麦抗叶锈病近等基因系材料TcLr45和其背景材料Thatcher进行PCR扩增,在TcLr45中获得了单一条带,而在Thatcher中则扩增出2条与其大小不同的条带。进一步利用49个小麦抗叶锈近等基因系进行扩增,经5%聚丙烯酰胺凝胶电泳分析,该片段为TcLr45特有片段。经TcLr45×Thatcher F2分离群体验证,该标记与Lr45基因连锁距离较远。对该片段进行克隆测序,片段长度为650 bp,与该引物在黑麦中的扩增序列有差异(643 bp),进一步经BLAST2对这2个序列分析,相似性为97%,表明该片段为由黑麦向小麦转移的外源片段。100个小麦品种检测中有16个品种扩增出与TcLr45相同的条带。
关键词:
黑麦,
抗叶锈基因,
Lr45,
SCAR标记
Abstract: A SCAR marker of rye genome was used to amplified the TcLr45 and the background material Thatcher． One unique band in TcLr45，and two different bands in Thatcher were implified． In addition， 49 wheat leaf rust resistance near isogenic gene lines( NILs) were detected by the marker， the marker is specific for TcLr45 among these genes． F2 segregate groups screening results showed a long distance genetic linkage between Lr45 and the marker． The band was cloned and sequenced， the full length 650 bp，which was different from the sequence amplified in rye ( 643 bp) ，compared the two sequences by BLAST2 soft， the homology is 97%． 16 of 100 wheat varieties were amplified the same bands as TcLr45
Key words:
Rye,
Leaf Rust resistance gene,
Lr45,
SCAR marker
中图分类号:
刘春燕, 郭楠, 闫红飞, 刘大群. 黑麦基因组特异SCAR标记检测抗叶锈相关黑麦染色质向小麦的转移[J]. 华北农学报, 2013, 28(3): 189-192. doi: 10.3969/j.issn.1000-7091.2013.03.034.
Liu Chunyan, GUO Nan, Yan Hongfei, Liu Daqun. Rye Genomic Specific SCAR Marker Detected Leaf Rust Resistance Related Gene in Rye Transferred to Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 189-192. doi: 10.3969/j.issn.1000-7091.2013.03.034.