日粮中添加小黑麦干酒糟对肉牛瘤胃5种代表性菌比例的影响

doi:10.7668/hbnxb.2014.02.019

华北农学报 ›› 2014, Vol. 29 ›› Issue (2): 98-103. doi: 10.7668/hbnxb.2014.02.019

所属专题： 畜牧；

日粮中添加小黑麦干酒糟对肉牛瘤胃5种代表性菌比例的影响

苏蕊¹, 杨钟², 丽春³, 张岩⁴, 赵鹏伟⁴, 武瑞兵⁵, 李金泉¹

1 内蒙古农业大学动物科学学院内蒙古呼和浩特 010018;
2 呼和浩特疾病预防控制中心内蒙古呼和浩特 010070;
3 内蒙古民族大学动物科学技术学院内蒙古通辽 028000;
4 内蒙古医科大学基础医学院内蒙古呼和浩特 010110;
5 内蒙古医科大学生物化学与分子生物学教研室内蒙古呼和浩特 010110

收稿日期:2013-10-12 出版日期:2014-04-28
通讯作者: 武瑞兵(1982-),男,内蒙古呼和浩特人,讲师,博士,主要从事分子遗传学研究。李金泉(1957-),男,内蒙古呼和浩特人,教授,博士生导师,主要从事绒山羊遗传育种研究。
作者简介:苏蕊(1980-),女,内蒙古巴彦淖尔人,讲师,博士,主要从事动物遗传育种研究。
基金资助:
内蒙古农业大学博士科研启动基金(BJ07-50);内蒙古自治区高等学校创新团队发展计划项目(NMGIRT1106)

Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets

SU Rui¹, YANG Zhong², LI Chun³, ZHANG Yan⁴, ZHAO Peng-wei⁴, WU Rui-bing⁵, LI Jin-quan¹

1 College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;
2 Huhhot Center for Control and Prevention, Huhhot 010070, China;
3 College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028000, China;
4 School of Preclinical Medicine, Inner Mongolia Medical University, Huhhot 010110, China;
5 Department of Biochemistry and Molecular Biology, Inner Mongolia Medical University, Huhhot 010110, China

Received:2013-10-12 Published:2014-04-28

摘要/Abstract

摘要： 旨在检测以小黑麦干酒糟及其可溶物(TDDGS)替代肉牛日粮中的所有粗料和部分精料对5种瘤胃代表性菌(Fibrobacter succinogenes、Succinovibrio dextrinosolvens、Selenomonas ruminantium、Megasphaera elsdenii和Prevotella ruminicola)比例的影响。利用Real-time PCR法检测了饲喂TDDGS前后5种瘤胃代表性菌数量百分比的变化。结果表明,30%TDDGS组与对照组相比,瘤胃中F.succinogenes、S.ruminantium和M.elsdenii菌数量百分比均有升高,分别显著升高3倍(P﹤0.05)、10倍(P﹤0.05)和2倍(P﹤0.05),而S.dextrinosolvens和P.ruminicola菌数量百分比却都降低了,但差异均不显著。

关键词: 肉牛, Real-time, PCR, 瘤胃微生物, 小黑麦, 干酒糟, 可溶物

Abstract: The aim of this study was to explore the effects of triticale dried distillers grains with solubles (TD- DGS) incorporation in beef cattle diets on the relative abundance of five rumen bacteria viz., Fibrobacter succinogenes, Succinovibrio dextrinosolvens, Selenomonas ruminantium, Megasphaera elsdenii and Prevotella ruminicola. Real- time PCR was used to detect the gene expression of these five rumen bacteria. As compared with control group, the results showed that the relative abundance of F. succinogenes, S. ruminantium and M. elsdenii in 30% TDDGS group increased, and a 3-fold (P < 0. 05), a 10-fold (P < 0. 05) and a 2-fold (P < 0. 05) significant increase were ob- served respectively, however, the relative abundance of S. dextrinosolvens and P. ruminicola decreased in 30% TD- DGS group but no significant differences were found.

Key words: Cattle, Real-time PCR, Rumen microorganisms, Triticale, Dried distillers grains, Solubles

中图分类号:

S858.23

苏蕊, 杨钟, 丽春, 张岩, 赵鹏伟, 武瑞兵, 李金泉. 日粮中添加小黑麦干酒糟对肉牛瘤胃5种代表性菌比例的影响[J]. 华北农学报, 2014, 29(2): 98-103. doi: 10.7668/hbnxb.2014.02.019.

SU Rui, YANG Zhong, LI Chun, ZHANG Yan, ZHAO Peng-wei, WU Rui-bing, LI Jin-quan. Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 98-103. doi: 10.7668/hbnxb.2014.02.019.

http://www.hbnxb.net/CN/Y2014/V29/I2/98

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日粮中添加小黑麦干酒糟对肉牛瘤胃5种代表性菌比例的影响

Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets

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日粮中添加小黑麦干酒糟对肉牛瘤胃5种代表性菌比例的影响

Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets

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