华北农学报 ›› 2011, Vol. 26 ›› Issue (1): 112-126. doi: 10.7668/hbnxb.2011.01.023

所属专题: 番茄 盐碱胁迫

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利用野生契斯曼尼高代回交群体定位番茄发芽期耐盐性QTLs

黄亚杰1,2, 杜永臣2, 侯喜林1, 王孝宣2, 高建昌2, 国艳梅2   

  1. 1. 南京农业人学园艺学院,江苏南京210095;
    2. 中国农业科学院蔬菜花卉研究所,北京100081
  • 收稿日期:2011-12-10 出版日期:2011-02-28
  • 通讯作者: 杜永臣(1955- ),男,河北昌黎人,研究员,博士生导师,主要从事番茄遗传育种研究。侯喜林(1960- ),男,河北张北人,教授,博士生导师,主要从事不结球自菜遗传育种及分子生物学研究。
  • 作者简介:黄亚杰(1985- ),女,河北沧州人,硕士研究生,主要从事番茄遗传育种研究。
  • 基金资助:
    国家自然科学基金(3077147430900988);国家“863”项目(2007AA10Z178);北京市自然科学基金项目(6102020)

QTLs Mapping of Salt Tolerance during Germination in a BCZS3 Population:Solanum lycopersicum 9706 Solanum cheesmanii

HUANG Ya-jie1,2, DU Yong-chen2, HOU Xi-lin1, WANG Xiao-xuan2, GAO Jian-chang2, GUO Yan-mei2   

  1. 1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-12-10 Published:2011-02-28

摘要: 以耐盐性差异显著的野生契斯曼尼番茄LA0317与栽培番茄9706为亲本材料,建立了包含130个BC2S3单株的高代回交群体,利用55个SSR标记和26个CAPS标记构建了番茄的遗传连锁图谱。采用多QTL区间分析法,检测到与发芽期耐盐性相关的9个QTLs。其中,利用24d发芽指数在第2,7,10,11染色体上检测到5个耐盐QTLs,利用24d发芽率在第1,3,8,12染色体上检测到4个QTLs。此外,研究获得了14个发芽期表型和QTLs分析均耐盐的株系,为利用野生契斯曼尼番茄创造优良耐盐种质资源奠定基础。

关键词: 番茄, 遗传连锁图谱, 发芽期, 发芽指数, 发芽率, QTL定位

Abstract: An advanced backross BCz S3 population consisted of 130 individuals was constructed from the crossbetween Solauum opersicum 9706 and Solauum cheesmauii LA0317.A total of 55 SSR and 26 CAPS markers wereused to construct a linkage map. Using MQM mapping method 9 QTLs of salt tolerance were detected during germina-tion stage totally among which 5 QTLs conferring germination index located nn chromosome 2,7,10,11 respectivelyand 4 QTLs conferring germination rate located nn chromosome 1 3 8,12 respectively. Fourteen families with high salttolerance were obtained which contained at least one of the major effect QTLs thus they could be used as breedingmaterial.

Key words: Tomato, Genetic map, Germination stake, Germination index, Germination rate, QTLs mapping

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引用本文

黄亚杰, 杜永臣, 侯喜林, 王孝宣, 高建昌, 国艳梅. 利用野生契斯曼尼高代回交群体定位番茄发芽期耐盐性QTLs[J]. 华北农学报, 2011, 26(1): 112-126. doi: 10.7668/hbnxb.2011.01.023.

HUANG Ya-jie, DU Yong-chen, HOU Xi-lin, WANG Xiao-xuan, GAO Jian-chang, GUO Yan-mei. QTLs Mapping of Salt Tolerance during Germination in a BCZS3 Population:Solanum lycopersicum 9706 Solanum cheesmanii[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 112-126. doi: 10.7668/hbnxb.2011.01.023.

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