摘要： 设计简并引物，用RT-PCR法从马铃薯野生种(Solanum pinnatisectum)克隆到了CHS(Chalcone synthase，查尔酮合酶)基因的全长cDNA，并用半定量RT-PCR法进行了表达分析。序列分析表明，CHS基因编码一个389个氨基酸残基的多肽，在氨基酸水平上与其他茄科植物的同源性达到89%～93%，多重比较和系统发育分析均表明该基因为CHS家族中的一个新成员；检测了CHS基因的空间表达模式，该基因在花和匍匐茎中表达量最高，在根和块茎中没有检测到，这与花和匍匐茎呈淡紫色有花色苷合成，而根和块茎呈白色无花色苷合成是相一致的；发现在块茎中CHS受光诱导表达，光照后的第3天表达量最高，而在光照前检测不到，光照前块茎为白色，随着光照时间的延长，其表层颜色因积累花色苷而变成紫红色。

关键词: 查尔酮合酶, 花色苷, cDNA克隆, 表达谱, 马铃薯野生种

Abstract: The degenerate primers were designed based on the published information of sequence of Solanaceae plants.The complete long cDNAs encodingCHSwas isolated from wild potatoes(Solanum pinnatisectum)by RT-PCR with the degenerated primers.The sequence analysis indicated thatCHScDNA encoded an polypeptide composed of 389 amino acid residues,the deduce protein showed 89%-93% identity withCHSof Solanaceae plant,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a member of the correspondingCHS-subfamily enzymes;The spatial expression patterns ofCHSgene in wild potato were examined by semi-quantitative RT-PCR with the specific primers.The expression analysis indicated thatCHSgene were preferentially expressed in flowers and stolons,it's tran-scripts could not detected in roots and tubers,and these were involved in accumulation of anthocyanins in slightly purple flowers and stolons,and no accumulation of anthocyanins in white roots and tubers.The response ofCHSgene expression was investigated by RT-PCR analysis in wild potato tubers to white light.The transcripts ofCHSgene were barely present or non-detected in the tuber skins before light induction,but light treatment increased their expression greatly.Expression ofCHSwas highest at 3 days after light exposure,and did not detected at 0.days after light exposure only,while tubers were only white before light treatment,it was indicated that the transcriptional activity ofCHSbasically correlate to antho-cyanin production in tuber skins.

Key words: Chalcone synthase, Anthocyanins, cDNA cloning, Expression pattern, Solanum pinnatisectum

中图分类号: 

• S532.01