摘要： 为探讨甘薯茎线虫的致病机理,对甘薯茎线虫分泌的蛋白类物质的致病作用进行了初步研究。使用5-methoxy-N,N-dimethyltryptamine和二甲亚砜刺激法刺激分泌腺生长,冰上研磨线虫破碎得到分泌颗粒,硫酸铵沉淀法和TCA/丙酮法获得其中的蛋白质进行甘薯片的致病性验证。结果表明,甘薯茎线虫食道腺分泌物尤其蛋白类物质具有明显的致病作用,食道腺中的蛋白类物质经硫酸铵沉淀法得到的粗蛋白比TCA/丙酮法提取的粗蛋白致病作用强,这种致病蛋白不耐高温也不抗紫外线照射,经100℃水浴30 min,或40 W在50 cm距离下紫外灯照射60 min后,几乎完全丧失了致病作用。

关键词: 甘薯茎线虫, 致病性, 分泌物, 蛋白质

Abstract: To explore Ditylenchus destructor Thorne pathogenic mechanism, a preliminary study was conducted withprotein secretions of D. destructor. After nematode glands growth was stimulated by 5 -methoxy -N, N -dimethyltryptamineand dimethyl sulfoxide, nemade was grinded on ice and secretory granules were acquired. The proteins from the granuleswere abstracted by TCA/Acetone and ammonium sulfate precipitation method. Then pathogenicity of protein was testedwith sweet potato slices.The results showed that the secretions from nematode esophageal gland, especially proteins obv- iously played a pathogenic role. Crude proteins abstracted by ammonium sulfate precipitation had the stronger pathogenicitythan that from TCA/acetone method. And the pathogenic crude proteins were heat sensitive and ultraviolet radiation sens- itive, they almost completely lost pathogenicity at 100? for 30min withwater bath and under 40w, 50 cm UV light expo -sure for 60 min.

Key words: Ditylenchus destructor, Pathogenicity, Secretion, Protein

中图分类号: 

• S435.313+.4