基于转录组测序技术挖掘大豆蛋白质合成相关基因

doi:10.7668/hbnxb.201751206

华北农学报 ›› 2019, Vol. 34 ›› Issue (1): 61-73. doi: 10.7668/hbnxb.201751206

所属专题： 油料作物；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

基于转录组测序技术挖掘大豆蛋白质合成相关基因

郭静文^1,2, 史晓蕾¹, 刘茜¹, 赵青松¹, 邸锐¹, 刘兵强¹, 闫龙¹, 王凤敏¹, 张孟臣¹, 赵宝华², 杨春燕¹

1. 河北省农林科学院粮油作物研究所, 国家大豆改良中心石家庄分中心, 农业部黄淮海大豆生物学与遗传育种重点实验室, 河北省遗传育种重点实验室, 河北石家庄 050035;
2. 河北师范大学生命科学学院, 河北石家庄 050024

收稿日期:2018-11-01 出版日期:2019-02-28
通讯作者: 杨春燕(1966-),女,河北石家庄人,研究员,硕士,主要从事大豆遗传育种研究;赵宝华(1967-),男,河北石家庄人,教授,博士,博士生导师,主要从事分子细菌学研究。
作者简介:郭静文(1992-),女,河北石家庄人,实习研究员,硕士,主要从事大豆遗传育种研究。
基金资助:
国家自然科学基金项目（31201234；31871652）；河北省青年拔尖人才计划、国家产业技术体系（CARS-004-PS06）；国家重点研发计划（2016YFD0100201）；河北省重点研发计划（16227516D）；现代农业科技创新工程项目（F17R37）

Transcriptome Analysis of Protein Synthesis Related Genes in Soybean

GUO Jingwen^1,2, SHI Xiaolei¹, LIU Qian¹, ZHAO Qingsong¹, DI Rui¹, LIU Bingqiang¹, YAN Long¹, WANG Fengmin¹, ZHANG Mengchen¹, ZHAO Baohua², YANG Chunyan¹

1. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang Branch of National Soybean Improvement Center, Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Hebei Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, China;
2. College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China

Received:2018-11-01 Published:2019-02-28

摘要/Abstract

摘要： 通过转录组测序技术挖掘蛋白质合成相关基因，为解析蛋白质合成机制奠定基础。以遗传背景相近但蛋白含量差异较大的大豆高蛋白品系冀HJ117（蛋白质含量52.99%）及其回交亲本冀豆12（蛋白质含量46.48%）为研究材料，利用转录组测序技术和生物信息学分析，以期挖掘大豆籽粒蛋白质合成相关基因。通过对转录组数据中冀HJ117和冀豆12差异表达基因的分析筛选，共得到336个差异表达基因，其中冀HJ117较冀豆12有195个上调表达基因，141个下调表达基因。通过GO功能富集分析，发现在分子功能类型中注释的差异基因主要与催化和连接等功能相关；KEGG显著富集分析发现差异表达基因主要富集在蛋白质内质网合成途径中，该途径共筛选到34个差异表达基因，其中33个基因在冀HJ117中表达量较高。从该途径中筛选出9个候选基因，采用荧光定量PCR方法检测其表达量，表达趋势与RNA-Seq检测结果基本一致，证实了RNA-Seq数据的可靠性。RNA-Seq测序结果获得了与蛋白质合成相关基因的基本信息，蛋白质内质网合成途径可能是冀HJ117与冀豆12蛋白质含量产生差异的重要通路。

关键词: 大豆, 蛋白质含量, 转录组测序, 差异表达基因, 荧光定量PCR

Abstract: By using transcriptome sequencing technology, the screening of protein synthesis related genes will lay a foundation for further exploring the mechanism of protein synthesis. Ji HJ117 (Protein content of 52.99%) and its backcross parent Jidou 12 (Protein content of 46.48%) with similar genetic backgrounds but different in protein content were used as research materials.Through RNA-sequencing of soybean seeds from Ji HJ117 and Jidou 12 to gain insights into the genes potentially related to protein synthesis. A total of 336 differentially expressed genes (DGEs) were screened from Ji HJ117 and Jidou 12, of which 195 were up-regulated and 141 down-regulated in Ji HJ117. Through the Gene Ontology analysis, DGEs annotated in molecular function types were mainly related to catalysis and ligation. Significant enrichment analysis of KEGG revealed that the DGEs were mainly enriched in protein processing in endoplasmic reticulum. A total of 34 DGEs were screened in this pathway, of which 33 genes were highly expressed in Ji HJ117. Nine candidate genes were selected from this pathway and their expression levels were detected by Real-time fluorescence quantitative PCR. The expression trend was basically consistent with that of RNA-Seq, confirming the reliability of RNA-Seq datas. RNA-Seq analysis obtained basic information about protein synthesis related genes in soybean. The pathway of protein processing in endoplasmic reticulum might be an important pathway that contribute to the difference of protein content in Ji HJ117 and Jidou 12. The accomplishment of these results constitute the first step toward understanding the regulation mechanisms of seed protein synthesis and provide valuable resource for further research.

Key words: Soybean, Protein content, Transcriptome sequencing, Differentially expressed genes, Fluorescent quantitative PCR

中图分类号:

S565.03
Q78

郭静文, 史晓蕾, 刘茜, 赵青松, 邸锐, 刘兵强, 闫龙, 王凤敏, 张孟臣, 赵宝华, 杨春燕. 基于转录组测序技术挖掘大豆蛋白质合成相关基因[J]. 华北农学报, 2019, 34(1): 61-73. doi: 10.7668/hbnxb.201751206.

GUO Jingwen, SHI Xiaolei, LIU Qian, ZHAO Qingsong, DI Rui, LIU Bingqiang, YAN Long, WANG Fengmin, ZHANG Mengchen, ZHAO Baohua, YANG Chunyan. Transcriptome Analysis of Protein Synthesis Related Genes in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 61-73. doi: 10.7668/hbnxb.201751206.

http://www.hbnxb.net/CN/Y2019/V34/I1/61

参考文献

[1] 赵团结, 盖钧镒. 栽培大豆起源与演化研究进展[J]. 中国农业科学, 2004, 37(7):954-962. doi:10.3321/j.issn:0578-1752.2004.07.004. Zhao T J, Gai J Y. The origin and evolution of cultivated soybean[J]. Scientia Agricultura Sinica, 2004, 37(7):954-962. doi:10.3321/j.issn:0578-1752.2004.07.004.
[2] 郑宇宏, 陈亮, 孟凡凡, 范旭红, 张云峰, 孙星邈, 王明亮, 王曙明. 吉林省不同年代大豆育成品种产量与品质性状变化趋势[J]. 东北农业科学, 2016, 41(6):45-49. doi:10.16423/j.cnki.1003-8701.2016.06.010. Zheng Y H, Chen L, Meng F F, Fan X H, ZhangY F, Sun X M, Wang M L, Wang S M. Changes of yield and quality traits of soybean cultivars released during different stages in Jilin Province[J]. Journal of Northeast Agricultural Sciences, 2016, 41(6):45-49. doi:10.16423/j.cnki.1003-8701.2016.06.010.
[3] 张伟, 王曙明, 邱强, 闫晓艳, 彭宝, 张晓霞, 姜海英. 从品种志分析吉林省八十五年来大豆育成品种产量和品质的演变[J]. 大豆科学, 2009, 28(6):970-975. doi:10.11861/j.issn.1000-9841.2009.06.970. Zhang W, Wang S M, Qiu Q, Yan X Y, Peng B, Zhang X X, Jiang H Y. Changes of yield and quality traits of released soybean cultivars during Past 85 Years in Jilin Province[J]. Soybean Science, 2009, 28(6):970-975. doi:10.11861/j.issn.1000-9841.2009.06.970.
[4] Hwang E Y, Song Q J, Jia G F, Specht J E, Hyten D L, Costa1 J, Cregan P B. A genome-wide association study of seed protein and oil content in soybean[J]. BMC Genomics 2014, 15:1. doi:10.1186/1471-2164-15-1.
[5] Bolon Y T, Joseph B, Cannon S B, Graham M A, Diers B W, Farmer A D, May G D, Muehlbauer G J, Specht J E, Tu Z J, Weeks N, Xu W W, Shoemaker R C, Vance C P. Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean[J]. BMC Plant Biology, 2010, 10:41. doi:10.1186/1471-2229-10-41.
[6] Kyujung Van, McHale L K. Meta-Analyses of QTLs associated with protein and oil contents and compositions in soybean9(Glycine max (L.) Merr.) seed[J]. International Journal of Molecular Sciences, 2017, 18(6):1180. doi:10.3390/ijms18061180.
[7] Patil G, Mian R, Vuong T, Pantalone V, Song Q, Chen P, Shannon G J, Carter T C, Nguyen H T. Molecular mapping and genomics of soybean seed protein:a review and perspective for the future[J]. Theoretical and Applied Genetics, 2017, 130(10):1975-1991. doi:10.1007/s00122-017-2955-8.
[8] 魏荷, 王金社, 卢为国. 大豆籽粒蛋白质含量分子遗传研究进展[J]. 中国油料作物学报, 2015, 37(3):394-410. doi:1007-9084.2015.03.021. Wei H, Wang J S, Lu W G. Molecular genetic advances in soybean seed protein[J]. Chinese Journal of Oil Crop Sciences, 2015, 37(3):394-410. doi:1007-9084.2015.03.021.
[9] Cober E R, Voldeng H D. Developing high-protein, high-yield soybean populations and lines[J]. Crop Science, 2000, 40(1):39-42. doi:10.2135/cropsci2000.40139x.
[10] Schmutz J, Cannon S B, Schlueter J, Ma J, Mitros T, Nelson W, Hyten D L, Song Q, Thelen J J, Cheng J, Xu D, Hellsten U, May G D, Yu Y, Sakurai T, Umezawa T, Bhattacharyya M K, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang X C, Shinozaki K, Nguyen H T, Wing R A, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker R C, Jackson S A. Genome sequence of the palaeopolyploid soybean[J]. Nature, 2010, 463(7278):178-183. doi:10.1038/nature08670.
[11] Phansak P, Soonsuwon W, Hyten D L, Song Q J, Cregan P B, Graef G L, Specht J E.Multi-Population selective genotyping to identify soybean[Glycine max (L.) Merr.] seed protein and oil QTLs[J]. G3 Genesgenetics, 2016, 6:1635-1648. doi:10.1534/g3.116.027656.
[12] Nichols D M, Glover K D, Carlson S R, Specht J E, Diers B W. Fine mapping of a seed protein QTL on soybean linkage group I and its correlated effects on agronomic traits[J]. Crop Science, 2006, 46(2):834-839. doi:10.2135/cropsci2005.05-0168.
[13] Chen J Q, Lang C X, Hu Z H. Antisense PEP gene to ratio of protein and lipid content in Brassica napus seeds[J]. Journal of Agricultural Biotechnology, 1999, 7(4):316-320. doi:10.1016/S0140-6736(69)91396-8.
[14] Wang H W, Zhang B, Hao Y J, Tian A G, Liao Y, Zhang J S, Chen S Y. The soybean Dof-type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plants[J]. The Plant Journal, 2007, 52(4):716-729. doi:10.1111/j.1365-313X.2007.03268.x.
[15] Verdier J, Thompson R D. Transcriptional regulation of storage protein synthesis during dicotyledon seed filling[J]. Plant Cell Physiol, 2008, 49(9):1263-1271. doi:10.1093/pcp/pcn116.
[16] Ezcurra I, Wycliffe P, Nehlin L, Ellerstrom M, Rask L. Transactivation of the Brassica napus napin promoter by ABI3 requires interaction of the conserved B2 and B3 domains of ABI3 with different cis-elements:B2 mediates activation through an ABRE, whereas B3 interacts with an RY/G-box[J]. Plant Journal, 2000, 24(1):57-66. doi:10.1046/j.1365-313x.2000.00857.x.
[17] 王楠. 谷子与锈菌互作的转录组和表达谱研究及相关基因表达分析[D].石家庄:河北师范大学, 2015. Wang N. The bac library construction and transcriptome analysis of wheat material which carry YR26 gene[D].Shijiazhuang:Hebei Normal University, 2015.
[18] Luan H Y, Shen H Q, Zhang Y H, Zang H, Qiao H L, Hong T, Chen J, Chen H. Comparative transcriptome analysis of barley(Hordeum vulgare L.) glossy mutant using RNA-Seq[J]. Brazilian Journal of Botany, 2016, 27(11):1-10. doi:10.1007/s40415-016-0328-1.
[19] Wang L, Liu L, Ma Y, Li S, Dong S, Zu W. Transcriptome profilling analysis characterized the gene expression patterns responded to combined drought and heat stresses in soybean[J]. Computation Biology Chemistry, 2018, 77:413-429. doi:10.1016/j.compbiolchem.2018.09.012.
[20] Wang Q Q, Liu F, Chen X S, Ma X J, Zeng H Q, Yang Z M. Transcriptome profiling of early developing cotton fiber by deep-sequencing reveals significantly differential expression of genes in a fuzzless/lintless mutant[J]. Genomics, 2010, 96(6):369-376. doi:10.1016/j.ygeno.2010.08.009.
[21] 和小燕, 胡晓锋, 王允, 张幸果, 李贺敏, 崔党群, 殷冬梅. 花生籽仁不同发育时期的转录组测序分析[J]. 分子植物育种, 2016, 14(11):2930-2943. He X Y, Hu X F, Wang Y, Zhang X G, Li H M, Cui D Q, Yin D M. Sequencing analysis of transcriptome during the different developmental stages in peanut seed[J]. Molecular Plant Breeding, 2016, 14(11):2930-2943.
[22] Julio V, Enrique I, Beatriz J,Martínez O, Vielle-Calzada J P, Herrera-Estrella L, Herrera-Estrella A. Deep sampling of the palomero maize transcriptome by a high throughput strategy of pyrosequencing[J]. BMC Genomics, 2009, 10(1):1-10. doi:10.1186/1471-2164-10-299.
[23] Alagna F, D'Agostino N, Torchia L, Servili M, Rao R, Pietrella M, Giuliano G, Chiusano M L, Baldoni L, Perrotta G. Comparative 454 pyrosequencing of transcriptsfrom two olive genotypes during fruit development[J]. BMC Genomics, 2009, 10(1):399-414. doi:10.1186/1471-2164-10-399.
[24] 栾海业, 臧慧, 沈会权,张英虎,乔海龙,陶红,申玉香. 大麦白化颖壳突变体的转录组学分析[J]. 核农学报, 2017, 31(12):2332-2339. Luan H Y, Zang H, Shen H Q, Zhang Y H, Qiao H L, Tao H, Shen Y X. Transcriptome analysis of albino lemma mutant in barley[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(12):2332-2339.
[25] 陈静. 花生种子休眠解除过程中相关基因转录组学研究[D].南京:南京农业大学,2004. Chen J. Analysis on transcriptome of genes involved in peanut seed dormancy release[D].Nanjing:Nanjing Agricultural University, 2004.
[26] 李海燕, 王芳, 段玉玺, 陈立杰. 大豆胞囊线虫侵染诱导五寨黑豆早期的转录组分析[J]. 中国油料作物学报, 2015, 37(2):194-200. doi:10.7505/j.issn.1007-9084.2015.02.011. Li H Y, Wang F, Duan Y X, Chen L J. Transcriptome analysis of Wuzhai heidou infected by Heterodera glycine[J]. Chinese Journal of Oil Crop Sciences, 2015, 37(2):194-200. doi:10.7505/j.issn.1007-9084.2015.02.011.
[27] 黄启秀, 曲延英, 姚正培,李梦雨,陈全家. 海岛棉枯萎病抗性与类黄酮代谢途径基因表达量的相关性[J]. 作物学报, 2017, 43(12):1791-1801. doi:10.3724/SP.J.1006.2017.01791. Huang Q X,Qu Y Y, Yao Z P, Li M Y, Chen Q J. Correlation between resistance to Fusarium wilt and expression of flavonoid metabolism related genes in Gossypium barbadense L.[J].Acta Agronimica Sinica,2017, 43(12):1791-1801. doi:10.3724/SP.J.1006.2017.01791.
[28] Cohen S P, Liu H, Argueso C T, Pereira A, Vera Cruz C, Verdier V, Leach J E. RNA-Seq analysis reveals insight into enhanced rice Xa7-mediated bacterial blight resistance at high temperature[J]. PLoS One, 2017,12(11):e0187625. doi:10.1371/journal.pone.0187625.
[29] 李永辉, 陈琳琳, 孙炳剑, 王利民,邢小萍,袁虹霞,丁胜利,李洪连. 假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析[J]. 作物学报, 2017, 43(11):1632-1642. doi:10.3724/SP.J.1006.2017.01632. Li Y H, Chen L L, Sun B J, Wang L M, Xing X P, Yuan H X, Ding S L, Li H L. Differential expression of three plant hormone related genes in wheat in-fected by Fusarium pseudograminearum[J]. Acta Agronmica Sinica,2017, 43(11):1632-1642. doi:10.3724/SP.J.1006.2017.01632.
[30] 曾庆东. 小麦抗条锈病基因Yr26 载体材料BAC文库构建及转录组测序[D].杨凌:西北农林科技大学,2016. Zeng Q D. The BAC library construction and transcriptome analysis of wheat material which carry Yr26 gene[J].Yangling:North West Agriculture and Forestry University,2016.
[31] 许家磊. 基于甘薯徐781和徐薯18转录组测序的SNP标记开发[D]. 北京:中国农业科学院, 2015. doi:10.7666/d.Y2787474. Xu J L. SNP marker development based on sequencing of sweet potato Xu 781 and Xushu 18 transcriptome[D]. Beijing:China Academic Journal Electronic Publishing House, 2015.doi:10.7666/d.Y2787474.
[32] 任梦露, 刘卫国, 刘婷, 杜勇利, 邓榆川, 邹俊林, 袁晋, 杨文钰. 荫蔽胁迫下大豆茎秆形态建成的转录组分析[J]. 作物学报, 2016, 42(9):1319-1331. doi:10.3724/SP.J.1006.2016.01319. Ren M L, Liu W G, Liu T, Du Y L, Deng Y C, Zou J L, Yuan J, Yang W Y. Transcriptome analysis of stem morphogenesis under shade stress in soybean[J]. Acta Agtonomica Sinica, 2016, 42(9):1319-1331.doi:10.3724/SP.J.1006.2016.01319.
[33] 孙爱清, 张杰道, 万勇善, 刘风珍, 张昆, 孙利. 花生干旱胁迫响应基因的数字表达谱分析[J]. 作物学报, 2013, 39(6):1045-1053. doi:10.3724/SP.J.1006.2013.01045. Sun A Q, Sun J D, Wan Y S, Liu F Z, Zhang K, Sun L. In silico expression profile of genes in response todrought in peanut[J]. Acta Agronomica Sinica, 2016, 39(6):1045-1053. doi:10.3724/SP.J.1006.2013.01045.
[34] Mortazavi A, Williams B A, Mccue K, Schaeffer L, Wold W. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods, 2008, 5(7):621-628. doi:10.1038/nmeth.1226.
[35] Audic S, Claverie J M. The significance of digital gene expression profiles[J]. Genome Research, 1997, 7(10):986-995. doi:10.1101/gr.7.10.986.
[36] 陈欢. 大豆籽粒不同发育时期基因表达谱的研究[D]. 长春:吉林农业大学, 2012. Chen H. Gene expression profile of developing soybean seed[D]. Changchun:Jilin Agricultural University, 2012.
[37] 杜若琛. 大豆籽粒发育过程中ABI3-like与贮藏蛋白基因的关系及调控机制的研究[D].晋中:山西农业大学,2016. Du R C. Study on the relationship between ABI3-like and storage protein genes during the development of soybean seeds and its regulation mechanism[D]. Jinzhong:Shanxi Agricultural University, 2016.
[38] Fuji K, Shimada T, Takahashi H, Tamura K, Koumoto Y, Utsumi S, Nishizawa K, Maruyama N, Hara-Nishimura I. Arabidopsis vacuolar sorting mutants(green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds[J]. Plant Cell, 2007, 19(2):597-609. doi:10.1105/tpc.106.045997.
[39] 韩宝达, 李立新. 植物种子贮藏蛋白质及其细胞内转运与加工[J]. 植物学报, 2010, 45(4):492-505. doi:10.3969/j.issn.1674-3466.2010.04.013. Han B D, Li L X. Seed storage proteins and their intracellular transport and processing[J]. Chinese Bulletin of Botany, 2010, 45(4):492-505.doi:10.3969/j.issn.1674-3466.2010.04.013.
[40] 张宁, 姜晶. 植物中小分子热激蛋白基因家族(sHSPs)研究进展[J]. 植物生理学报, 2017(6):943-948. doi:CNKI:SUN:ZWSL.0.2017-06-006. Zhang N, Jiang J. Research advances of small heat shock protein gene family (sHSPs) in plants[J]. Plant Physiology Journal, 2017, 53(6):943-948. doi:CNKI:SUN:ZWSL.0.2017-06-006.
[41] 王义菊. 过量表达小分子热激蛋白对番茄耐热性的影响[D]. 济南:山东师范大学, 2005. Wang Y J. The effects of overexpression of small shock proteins on the thermotolerence in tomato[D]. Jinan:Shandong Normal University, 2005.
[42] Molinari M. N-glycan structure dictates extension of protein folding or onset of disposal[J]. Nature Chemical Biology, 2007, 3(6):313-320. doi:10.1038/nchembio880.
[43] Soldà T, Galli C, Kaufman R J, Molinari M. Substrate-specific requirements for UGT1-dependent release from calnexin[J]. Molecular Cell, 2007, 27(2):238-249. doi:10.1016/j.molcel.2007.05.032.
[44] Hebert D N, Foellmer B, Helenius A. Glucose trimming and reglucosylation determine glycoprotein association with calnexin in the endoplasmic reticulum[J]. Cell, 1995, 81(3):425-433. doi:10.1016/0092-8674(95)90395-x.
[45] Hirsch C, Gauss R, Horn S C, Neuber O, Sommer T. The ubiquitylation machinery of the endoplasmic reticulum[J]. Nature, 2009, 458(7237):453-460. doi:10.1038/nature07962.
[46] Gurkan C, Stagg S M, Lapointe P, Balch W E. The COPⅡ cage:unifying principles of vesicle coat assembly[J]. Nature Reviews Molecular Cell Biology, 2006, 7(10):727-738. doi:10.1038/nrm2025.
[47] Younger J M, Chen L, Ren H Y, Rosser M F, Turnbull E L, Fan C Y, Patterson C, Cyr D M. Sequential quality-control checkpoints triage misfolded cystic fibrosis transmembrane conductance regulator[J]. Cell, 2006, 126(3):571-582. doi:10.1016/j.cell.2006.06.041.
[48] 吴迪. 内质网中FKBP23与BiP结合后对BiP的ATPase酶活性的影响[D]. 天津:南开大学, 2005. doi:10.7666/d.y804786. Wu D. Effect of binding of FKBP23 to BiP on ATPase activity of BiP in endoplasmic reticulum[D].Tianjin:Nankai University, 2005. doi:10.7666/d.y804786.
[49] Li P S, Yu T F, He G H, Chen M, Zhou Y B, Chai S H, Xu Z S, Ma Y Z. Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf34 involvement in drought and heat stresses[D].BMC Genomics, 2014,15(1):1-16. doi:10.1186/1471-2164-15-1009.
[50] Chauhan H, Khurana N, Agarwal P, Khurana J P, Khurana P. A seeds preferential heat shock transcription factor from wheat provides abiotic stress tolerance and yield enhancement in transgenic Arabidopsis under heat stress environment[J]. PloS One, 2013, 8(11):e79577. doi:10.1371/journal.pone.0079577.

选择文件类型/文献管理软件名称

选择包含的内容

基于转录组测序技术挖掘大豆蛋白质合成相关基因

Transcriptome Analysis of Protein Synthesis Related Genes in Soybean

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	贾建平, 李金鸿, 于敬文, 余曦玥, 彭德良, 李惠霞, 黄文坤. 氮肥对控制大豆孢囊线虫病危害的影响[J]. 华北农学报, 2022, 37(4): 198-205.
[2]	张斌. *大豆GmPP2C89基因在盐胁迫中的功能分析*[J]. 华北农学报, 2022, 37(4): 20-27.
[3]	张晋玉, 徐新娟, 晁毛妮, 张晓红, 吴向远, 高际涛, 黄中文. *大豆GmZAT12基因的克隆和表达分析*[J]. 华北农学报, 2022, 37(3): 1-7.
[4]	吉颖, 孙枫, 吴淑华, 李硕, 涂丽琴, 高丹娜, 崔晓艳, 陈新, 季英华, 郭青云. 江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析[J]. 华北农学报, 2022, 37(3): 200-205.
[5]	安清明, 王星, 吴震洋, 孟金柱, 赵园园, 宋兴超. 基于转录组挖掘不同性别贵州白山羊肌肉生长发育的关键基因及PI3K/ATK信号通路分析[J]. 华北农学报, 2022, 37(3): 213-222.
[6]	张敬敬, 李冰, 史宇凡, 高秀瑞, 潘秀清, 宋雪, 武彦荣. 不同硬度西瓜果皮的转录组测序及相关基因表达分析[J]. 华北农学报, 2022, 37(3): 44-52.
[7]	李艳燕, 赵彩桐, 齐阳阳, 刘明, 张书瑞, 刘志华, 李文滨, 姜振峰. 大豆主茎木质素积累规律分析[J]. 华北农学报, 2022, 37(3): 77-85.
[8]	史建磊, 熊自立, 苏世闻, 王克磊, 宰文珊. 基于RNA-seq的番茄青枯病响应基因鉴定与表达分析[J]. 华北农学报, 2022, 37(2): 171-182.
[9]	魏亚蕊, 赵曙良, 成晓华, 闫琦, 刘娜, 张玉星. 水杨酸诱导鸭梨抗黑斑病效果及机理的探讨[J]. 华北农学报, 2022, 37(2): 183-191.
[10]	韩晓勇, 蒋璐, 殷剑美, 金林, 张培通. 山药品种苏蓣8号抗炭疽病基因的转录组测序与表达分析[J]. 华北农学报, 2022, 37(2): 211-222.
[11]	王晓丽, 王敏, 岳爱琴, 郭数进, 王鹏, 王利祥, 杨婷婷, 张海生, 张永坡, 高春艳, 张武霞, 牛景萍, 杜维俊, 赵晋忠. 氮素营养和根瘤菌接种对大豆结瘤固氮和生长的影响[J]. 华北农学报, 2022, 37(1): 95-102.
[12]	李曼, 史晓蕾, 邸锐, 刘志芳, 孟庆民, 付才, 杨春燕, 王冬梅, 张孟臣, 张洁, 闫龙. 大豆长片段插入/缺失标记的开发与应用[J]. 华北农学报, 2022, 37(1): 18-26.
[13]	韦锦坚, 覃潇敏, 农玉琴, 骆妍妃, 陆金梅, 陈远权, 韦持章. 茶与大豆间作对土壤微生物群落代谢功能多样性的影响[J]. 华北农学报, 2021, 36(S1): 289-296.
[14]	王大刚, 陈圣男, 黄志平, 李杰坤, 吴倩, 胡国玉, 王维虎, 杨永庆. 大豆新品系抗SMV鉴定及分析[J]. 华北农学报, 2021, 36(S1): 326-332.
[15]	高倩, 冯燕, 杨雅华, 赵青松, 雷雅坤, 刘兵强, 张孟臣, 史晓蕾, 杨春燕. 基于全基因组关联分析挖掘野生大豆蛋白含量QTL[J]. 华北农学报, 2021, 36(S1): 23-30.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

基于转录组测序技术挖掘大豆蛋白质合成相关基因

Transcriptome Analysis of Protein Synthesis Related Genes in Soybean

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价