摘要： 以186个花椰菜杂交品种及组合为试材, 进行了游离小孢子培养研究。结果表明: 基因型是胚状体发生的决定因素; 24 h 32℃的高温前处理是胚状体发生的必须条件; 单核靠边期到双核期的小孢子是进行胚状体诱导的最佳时期, 胚状体发生频率最高; 培养基中添加6-BA 0.1 mg/L, NAA 0.005 mg/L可以促进胚状体发育。将20 d的胚状体进行脱分化处理, 可以大幅度提高胚状体的成活率, 植株再生率为53%, 再生植株中双单倍体占83.5%。

关键词: 花椰菜, 游离小孢子培养, 植株再生, 双单倍体

Abstract: The cultivation of isolated microspores with 186 caulfflowers cross cultivate and combinations were studieb. The results showed that genotype was a critical factor affecting embryogenesis in isolated microspore culture. The heat shock pretreatmern at 32 ℃ for 24 h was necessary for embryo formation; the stage from uninuclear to binuclear was the optimum period for embryo inocanent and the highest frequency of anbryogenesis could be attained; it also indicated that the addition of 0.1 mg/ L CUBA and 0.005 mg/L NAA to culture medium could promote embryoid development. The survival rate of 20-day embryo was greatly enhanced when treatebwith dedifferentiation. Consequernly, the ratio of plant re-generation was 53%, the ratio of DHin regenerate plarn was 83.5%.

Key words: Cauliflower, Isolated-microspore culture, Plant regeneration, Double haploid

中图分类号: 

• S634