摘要： 通过对冬小麦花培1代至花培5代的品质测试, 研究了花培品质育种的品质测试的世代程序和有效性。结果表明, 品质性状在花培早代已趋于稳定, 高分子量谷蛋白亚基组成从H1起就稳定遗传, 115对H₁与H₂材料和62对H₂与H₃材料的籽粒蛋白质含量、硬度和沉降值具有高度相关性, 且籽粒蛋白质含量和SDS沉降值H₂与H₃世代间差异不显著。对中选的京单00-594的H₄和H₅世代进一步品质测定结果表明, 通过花培早代选出的优质材料是可靠的, 其优质性状能稳定遗传。研究提出了花培品质育种各世代品质测试的程序, 证明了花药培养结合早代品质测试, 是一条快速、有效的小麦品质育种途径。

关键词: 冬小麦, 花药培养, 品质测试

Abstract: In this paper, we systematically tested the quality characteritics of wheat double haploid(DH)lines from the first generation(H₁) to H₅, with the aim to breed elite winter wheat lines with superior grain quality trait. We found that the composition of high molecular weight glutenin subunits did not change from H₁ to H₅. By contrast, seed protein content varied significantly between H₁ to H₂. Additional analysis revealed the existence of high conelation betweenthe test results of H₂ and H₃ generations with respect to seed protein content, grain hardness and SDS sedimentation volume of dough. Through early tests of quality characteristics, a DH line(Jingdan 00-594) shoving superior grain quality trait was developed. Its grain and dough properties benene stable in as early as H₄ and H₅ genenations. Based on our data, an effective protocol for the early and efficient selection of the DH lines with elite grain quality trait is proposed and discussed.

Key words: Winter wheat, Anther culture, Quality test

中图分类号: 

• S512.1

• S336