摘要： 小果雪兔子分布于新疆帕米尔高原，是一种濒临灭绝植物。利用组织培养的方法研究和保存珍稀的种质资源，是一种有效途径。以小果雪兔子无菌苗下胚轴为外植体，在含有6_BA（0.2 mg/L）和NAA（2 mg/L）的MS固体培养基上，20 d后可诱导出紫红色愈伤组织。当经4℃处理的愈伤组织转移到含6_BA（2 mg/L）+NAA（0.2 mg/L）+水解乳蛋白（250 mg/L）的MS培养基上，25 d后在愈伤组织上即可诱导出芽，出芽率为240%，继代培养后形成丛生苗，增殖率可达3~5倍。将2~3cM长的幼苗，转移到IAA（0.5 mg/L）+活性炭（0.5%）的1/2MS固体培养基上，20 d即可从茎基部生根，生根率74%。

关键词: 小果雪兔子, 组织培养

Abstract: Saussurea simpsoniana was a kind of plant which was close to die out.It is an effective approach that researched and preserved the sources of species by tissue culture.In this experiment,Saussurea simpsoniana callus was induced from the hypocotyls of sterile seedling,which collected from the Pamirs in XinJiang and was cultured on solid MS medium contained 0.2(mg/L) 6-BA and 2(mg/L) NAA for 20 days.Only pink Callus had differentiation capability.Adventitious buds were induced from pink Callus,which were disposed in 4℃,on solid MS medium contained 6-BA 2(mg/L) and NAA 0.2 mg/L and 250 mg/L Lactalbumin Hydrolysate for 25 days,the buding rate was 240%.Cluster shoots were set up through subculturing.The propagation times were up to 3-5.When the shoot developed 2-3cM Length,they were cultured on MS medium supplemented with 0.5 mg/L IAA and 0.5% active carbon for 20 days.Roots were formed from cauline base,then the rate of rooting was up to 74%.

Key words: Saussurea simpsoniana, Tissue culture

中图分类号: 

• S949.783.5