摘要： 对普通小麦-Aegilops crassa核质杂种及其核亲本灌浆期旗叶过氧化物酶和酯酶同工酶进行了研究,结果表明:3个测试核亲本的过氧化物酶同工酶的谱带数和带有Aegilops crassa细胞质的3个核质杂种的酶谱数不尽一致,但其中谱带1的酶活性均以弱势表达;核质杂种的酯酶同工酶与相应的核亲本比较酶带数目变化不明显,只是存在活性上的差异,这种差异在不同测试材料间也并未发现规律性的变化,表现了不同的核质杂种灌浆期旗叶过氧化物酶与酯酶同工酶的活性有所不同.说明Aegilops crassa 细胞质与普通小麦的细胞核结合产生的特异核质互作,在灌浆期旗叶的有关同工酶上也有所体现.
关键词:
普通小麦,
Aegilops crassa,
核质杂种,
同工酶
Abstract: Isoenzymes of Peroxidase and Esterase were analyzed in flag leaf from 3 group′s alloplasmic common wheats with Aegilops crassa 6x cytoplasm. The bands number of peroxidase isoenzymes is 8 for Norin 26,7 for Yongliang 4 and 6 for CAU-wu-01,while the number of peroxidase isoenzyme′s bands of 3 NC hybrids with Aegilops crassa 6x cytoplasm were all the same,The isoenzyme activity for band 1 expressed weakly in all the NC hybrids wheats.Compared with nuclear donor parents, the number of esterase bands did not show change obviously but the activity of the enzyme showed a bit different.The esterase activity among different supplied materials did not change regularly.All these results show that the interaction of Aegilops crassa 6x cytoplasm and common wheat nucleus can be expressed by relative activity of isoenzymes in flag leaves at filling stage.
Key words:
Common wheat,
Aegilops crassa,
NC-hybrid,
Isoenzyme
中图分类号:
吴晓华, 张爱民, 李元清, 刘冬成, 于美玲. 普通小麦-Aegilopscrassa核质杂种的同工酶研究[J]. 华北农学报, 2003, 18(4): 5-8. doi: 10.3321/j.issn:1000-7091.2003.04.002.
WU Xiao-hua, ZHANG Ai-min, LI Yuan-qing, LIU Dong-cheng, YU Mei-ling. Study on Isoenzymes of Alloplasmic Common Wheats with Aegilops crassa Cytoplasm[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 5-8. doi: 10.3321/j.issn:1000-7091.2003.04.002.