华北农学报 ›› 2009, Vol. 24 ›› Issue (6): 210-214. doi: 10.7668/hbnxb.2009.06.043

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八个榆黄蘑菌株同功酶分析

张玉铎, 李明, 张晓倩, 李守勉, 田景花   

  1. 河北农业大学, 园艺学院,河北 保定 071001
  • 收稿日期:2009-08-24 出版日期:2009-12-28
  • 通讯作者: 李明(1961-),男,河北故城人,教授,主要从事食用菌生物技术与遗传育种研究。
  • 作者简介:张玉铎(1982-),男,河北威县人,在读硕士,主要从事食用菌生物技术与遗传育种研究.
  • 基金资助:
    国家自然基金(30871713)

Study on the Isozymes of 8 Different Pleurotus citrinopileatus Strains

ZHANG Yu-duo, LI Ming, ZHANG Xiao-qian, LI Shou-mian, TIAN Jing-hua   

  1. College of Horticulture, Hebei Agricultural University, Baoding 071001, China
  • Received:2009-08-24 Published:2009-12-28

摘要: 为了探讨不同榆黄磨菌株之间的亲缘关系.采用垂直板聚丙烯酰胺凝胶电泳技术,利用酯酶同工酶和过氧化物同工酶酶谱的相似性和差异性,计算了8个榆黄蘑供试菌株间的Pearson相似系数;并采用类间平均法(Between-groups linkage)进行系统聚类,对供试菌株的亲缘关系进行分析.结果表明,所有供试菌株在2个同工酶系统中均有共同特征谱带,同时又体现了一定的多态性.菌株pc5与其他各菌株遗传差异均较大;菌株pc2与菌株pc7的遗传差异很小,可能是源于同一种或为亲缘关系很近的变异类型;其余各菌株都有一定程度的差异性.

关键词: 榆黄蘑, 同工酶, 亲缘关系, 聚类分析

Abstract: The band differences and similarity of esterase isoenzymes(EST) and peroxidase isoenzymes (POD) of 8 pleurotus citrinopileatus strains were analysed via polyacrylamid gel electrophoresis (PAGE)technique.The pearson correlation of the test materials were calculated and cluster dendrogoam for 8 strains was drawn by using betweengroups linkage cluster method.The experimental results showed that the basic characteristics of the isozyme bandswere observed and the polymorphism was also detected with two isozymes.The relationship of strains pc5 with others was remote.The relationship between strains of pc-and pc-7 was very close.

Key words: Pleurotus citrinopileatus, Isoenzyme, Relationship, Cluster analysis

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引用本文

张玉铎, 李明, 张晓倩, 李守勉, 田景花. 八个榆黄蘑菌株同功酶分析[J]. 华北农学报, 2009, 24(6): 210-214. doi: 10.7668/hbnxb.2009.06.043.

ZHANG Yu-duo, LI Ming, ZHANG Xiao-qian, LI Shou-mian, TIAN Jing-hua. Study on the Isozymes of 8 Different Pleurotus citrinopileatus Strains[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 210-214. doi: 10.7668/hbnxb.2009.06.043.

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