早熟油桃紫金红1号亲本的SSR鉴定

doi:10.7668/hbnxb.2009.06.042

华北农学报 ›› 2009, Vol. 24 ›› Issue (6): 205-209. doi: 10.7668/hbnxb.2009.06.042

早熟油桃紫金红1号亲本的SSR鉴定

沈志军, 马瑞娟, 俞明亮, 宋宏峰, 蔡志翔

江苏省农业科学院, 园艺研究所,江苏南京 210014

收稿日期:2009-09-03 出版日期:2009-12-28
通讯作者: 俞明亮(1965-),男,江苏姜堰人,研究员,博士,主要从事桃种质资源和育种研究。
作者简介:沈志军(1978-),男,江苏如皋人,助研,硕士,主要从事桃种质资源收集整理和育种研究.
基金资助:
国家自然基金(30871681);国家"863"项目(2006AA100108);国家科技基础条件平台项目(2005DKA21002-21);江苏省科技基础设施建设计划(BM2008008);现代农业产业技术体系建设资金(nycytx-31).

Parental Analysis of New Nectarine Variety Zijinhong1 by SSR

SHEN Zhi-jun, MA Rui-juan, YU Ming-liang, SONG Hong-feng, CAI Zhi-xiang

Institute of Horticulture, Jiangsu Academy of Agriculture Science, Nanjing 210014, China

Received:2009-09-03 Published:2009-12-28

摘要/Abstract

摘要： 油桃新品种紫金红1号是从早熟油桃中实生选育的,果实经济性状优良.以23个疑似亲本的油桃品种为试材,利用SSR标记共显性的特点,进行了紫金红1号亲本鉴定.SSR-PCR扩增结果表明,163对SSR引物中有48对能在试材中扩增出多态性良好、谱带清晰的产物,其中17对SSR引物扩增出的20个SSR位点呈现共显性带型.基于18个SSR等位基因信息的亲缘关系分析发现,紫金红1号与早红2号之间的亲缘关系最近,相似系数为0.881,在树状图上也聚为一类.经共显性SSR位点遗传规律分析,使用3个SSR引物(CPPCT1、BPPCT031、UDP96-018)的扩增位点,可排除早红2号以外的其他品种作为紫金红1号亲本的可能.认为早红2号自交获得紫金红1号可能性很大,或至少是其亲本之一.

关键词: 油桃, SSR, 亲本鉴定

Abstract: New nectarine variety Zijinhong1 was selected from naturally pollinated seedlings of early matured nec tarine because of its superior quality in economic traits.However,its parents were still unknown.Twenty three nectarine varieties which were suspectedto be its parents,aswell as Zijinhong1 itself,were used as plant material,and SSRmarkers for its codominant characteristic were used to analyze the probable parents.The results of SSRPCR showed that 48 primersout of 163 could produce polymorphism and legible alleles.Twenty loci amplified by 17 primers performed co dominant pattern.Genetic relationship based on 185 alleles showed that EarlyRed 2was the most similar variety to Zijin hong1 with a SM coefficient of 0.881,and the two varieties were clustered into one group in dendrograme.According to the statistic of inheritance of codominant loci,three loci amplified by CPPCT15,BPPCT031 andUDP96018,respective ly,could dispose all tested varieties possibly to be the parents of Zijinhong1 except EarlyRed 2.So Zijinhong1 may most probably selected from self cross seedlings of EarlyRed 2,or EarlyRed was at least one parent of Zijinhong1.

Key words: Nectarine, SSR, Parental analysis

中图分类号:

S662.03

沈志军, 马瑞娟, 俞明亮, 宋宏峰, 蔡志翔. 早熟油桃紫金红1号亲本的SSR鉴定[J]. 华北农学报, 2009, 24(6): 205-209. doi: 10.7668/hbnxb.2009.06.042.

SHEN Zhi-jun, MA Rui-juan, YU Ming-liang, SONG Hong-feng, CAI Zhi-xiang. Parental Analysis of New Nectarine Variety Zijinhong1 by SSR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 205-209. doi: 10.7668/hbnxb.2009.06.042.

http://www.hbnxb.net/CN/Y2009/V24/I6/205

参考文献

[1] 俞明亮,马瑞娟,杜平,等.早熟油桃新品种--紫金红1号的选育[J].果树学报,2008,25(1):134-135.
[2] Aranzana M J,Arus P,Carbo J.AFLP and SSR markers for genetic diversity analysis and cultivar identification in peach[Prunus persica (L.) Batsch[J].Acta Horticulture,2001,546:367-370.
[3] Arus P,Aranzana M J,Carbo J.SSR and AFLP markers for germplasm evaluation and cultivar identification in peach[J].Acta Horticulturae,2003,606:35-40.
[4] Aranzana M J,Carbo J,Arus P.Microsatellite variability in peach[Prunus persica (L.) Batsch]:cultivar identification,marker mutation,pedigree inferences and population structure[J].Theor Appl Genet,2003,106(8):1341-1352.
[5] Cheng H Y,Yang W C,Hsiao J Y.Genetic diversity and relationship among peach cultivars based on Random Amplified Microsatellite Polymorphism (RAMP)[J].Botanical Bulletin of Academia Sinica,2001,42(3):201-206.
[6] Martinez Gomez P,Arulsekar S,Potter D.Relationships among peach,almond,and related species as detected by simple sequence repeat markers[J].Journal of the American Society for Horticultural Science,2003,128(5):667-671.
[7] Lu Zhenxiang,Sosinski B,Reghard G L,et al.Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks[J].Genome,1998,41:199-207.
[8] Bielenberg D G,Wang Y,Fan S,et al.A deletion affecting several gene candidates is present in the Evergrowing peach mutant[J].Journal of Heredity,2004,95(5):436-444.
[9] Yamamoto T,Shimada T,Imai T,et al.Characterization of morphological traits based on a genetic linkage map in peach[J].Breeding Science,2001,51(4):271-278.
[10] Pancaldi M,Kacer Y,Kuden A B,et al.Using microsatellite sequence from peach for fingerprinting and genealogical analysis of almond[J].Rivista di Frutticolturae di Ortofloricoltura,1999,161(11),70-73.
[11] Yamamoto T,Mochida K,Hayas hi T.Shanghai suimitsuto,one of the origins of Japanese peach cultivar[J].Journal of the Japanese Society for Horticultural Science,2003,72(2):116-121.
[12] Yamamoto T,Mochida K,Imai T,et al.Parentage analysis in Japanese peaches using SSR markers[J].Breeding Science,2003,53(1):35-40.
[13] Dirlewanger E,Cosson P,Tavaud M,et al.Development of microsatellite markers in peach[Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry[J].Theor Appl Genet,2002,105:127-138.
[14] 俞明亮,马瑞娟,沈志军,等.桃果肉颜色、果皮茸毛和花粉育性性状的分子标记[J].园艺学报,2006,33 (3):511-517.
[15] Aranzana M J,Garica Mas J,Carbo J,et al.Development and variability analysis of microsatellite markers in peach[J].Plant Breeding,2002,121:1,87-92.
[16] Cipriani G,Lot G,Huang Wg,et al.AC/GT and AG/CT microsatellite repeat in peach[Prunus persica (L) Batsch]:isolation,characterization and cross-species amplification in Prunus[J].Theor Appl Genet,1999,99:65-72.
[17] Sosinski B,Gannavarapu M,Hager L D,et al.Characterization of microsatellite markers in peach[Prunus persica (L.) Batsch] [J].Theor Appl Genet,2000,101:421-428.
[18] 沈志军,马瑞娟,俞明亮,等.桃SSR-PCR主要因子对扩增产物的影响[J].江苏农业学报,2006,22(1):76-78.
[19] Hormaza J I.Molecular characterization and similarity relationships among apricot (Prunus Armeniaca L.) genotypes using simple sequence repeats[J].Theor Appl Genet,2002,104:321-328.
[20] Marilyn L,Warburton,and Fredrick A B.Genetic diversity in peach (Prunus persica L.Batch) revealed by Randomly Ampilfied Polymorphic DNA(RAPD) markers and compared to inbreeding coefficients[J].J Amer Soc HORT SCI,1996,12(6):1012-1019.

选择文件类型/文献管理软件名称

选择包含的内容

早熟油桃紫金红1号亲本的SSR鉴定

Parental Analysis of New Nectarine Variety Zijinhong1 by SSR

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	伊六喜, 邬阳, 曹彦, 贾海滨, 斯钦巴特尔, 高凤云, 周宇, 贾霄云. 亚麻主要产量相关性状的SSR关联分析[J]. 华北农学报, 2022, 37(5): 52-61.
[2]	凌晨, 赵洪, 颜军, 马莹雪, 冯艳芳, 张靖立, 李波, 邓超, 徐振江. 基于SSR标记的莴苣品种鉴定体系的建立及应用[J]. 华北农学报, 2022, 37(4): 34-44.
[3]	张馨方, 张树航, 李颖, 郭燕, 王广鹏. 基于SSR标记构建燕山板栗核心种质[J]. 华北农学报, 2021, 36(S1): 31-38.
[4]	董丽, 石海春, 赵长云, 余学杰, 柯永培. 玉米矮秆突变体K718d的遗传鉴定[J]. 华北农学报, 2021, 36(6): 71-77.
[5]	李靓, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇, 刘宇飞. 6个马铃薯新品系的细胞学及SSR分析[J]. 华北农学报, 2021, 36(2): 74-80.
[6]	伊六喜, 萨如拉, 王树彦, 李志伟, 斯钦巴特尔, 李强. 亚麻木酚素含量的SSR关联分析[J]. 华北农学报, 2021, 36(1): 63-70.
[7]	张久坤, 齐阳阳, 李立竹, 宁哓霜, 刘志华, 姜振峰, 李文滨. 利用BSA法定位大豆全基因组株高QTL及候选基因分析[J]. 华北农学报, 2020, 35(S1): 1-10.
[8]	吕冰冰, 代畅, 彭正松, YAMAMOTO Naoki, 吴一超, 魏淑红, 杨在君. 一组新的小麦EST-SSR标记开发及其在遗传图谱构建中的应用[J]. 华北农学报, 2020, 35(4): 57-63.
[9]	易红梅, 任洁, 王璐, 王蕊, 葛建镕, 王凤格, 赵久然, 徐明良. 2014-2019年国家玉米区域试验参试组合DNA指纹检测及遗传多样性分析[J]. 华北农学报, 2020, 35(3): 87-93.
[10]	李祥栋, 石明, 陆秀娟, 潘虹, 魏心元, 陆平. 利用叶绿体基因组SSR标记揭示薏苡属种质资源的遗传多样性[J]. 华北农学报, 2019, 34(S1): 6-14.
[11]	宁宇, 郑彦超, 武高洁, 王义飞, 张曼胤. 扁秆荆三棱不同组织混合样品的转录组分析[J]. 华北农学报, 2019, 34(6): 74-81.
[12]	郭慧娟, 贾举庆, 李欣, 乔麟轶, 阎晓涛, 任永康, 常利芳, 张树伟, 畅志坚, 张晓军. 小麦种质CH7015中抗白粉病基因的SSR定位[J]. 华北农学报, 2019, 34(6): 203-208.
[13]	李小军, 徐鑫, 张自阳, 刘明久, 茹振钢. 小麦2B染色体新SSR标记开发[J]. 华北农学报, 2019, 34(3): 68-74.
[14]	贾小平, 张博, 李剑峰, 袁玺垒, 陆平, 戴凌峰. 谷子SSR标记与光周期敏感性的关联分析[J]. 华北农学报, 2019, 34(1): 89-96.
[15]	张素君, 唐丽媛, 李兴河, 王海涛, 刘存敬, 张香云, 张建宏. SSR标记与陆地棉田间黄萎病抗性的关联分析[J]. 华北农学报, 2018, 33(6): 152-159.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

早熟油桃紫金红1号亲本的SSR鉴定

Parental Analysis of New Nectarine Variety Zijinhong1 by SSR

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价