扁秆荆三棱不同组织混合样品的转录组分析

doi:10.7668/hbnxb.201751606

摘要/Abstract

摘要： 扁秆荆三棱是一种常见的稻田杂草，旨在补充其转录组信息，为相关防治工作提供支持。基于高通量测序技术，在Illumina Solexa HiSeq 2000平台上对扁秆荆三棱的茎、叶、根茎和球茎的混合样品进行转录组分析。经拼接组装共获得了59 788个Unigene，序列的平均长度842 bp，N50为1 402 bp。将获得的Unigene与5个通用公共数据库（NR、Swiss-Prot、KEGG、GO、KOG）进行比对（Evalue<1e-5），35 221条Unigene获得了基因注释，占总Unigene的58.91%。在12 823个Unigene中共搜索到9 698个SSR位点，其中二核苷酸和三核苷酸的重复类型占所有SSR位点的95.93%。通过KEGG pathways分析，共有13 141个Unigene参与了291个代谢通路，获得了扁秆荆三棱淀粉合成功能相关Unigene 60个，根茎生长功能相关Unigene 14个。其中赤霉素相关基因、核糖体代谢通路等反映了地下根茎网络系统的扩张趋势以及活跃的能量需求，淀粉合成基因则说明其球茎中的营养储备开始于地下系统扩张的早期，这些发现为深入研究地下根茎网络调控机制，或杂草防治或湿地保护等实践工作提供了参考。

关键词: 扁秆荆三棱, 高通量测序, 转录组, 基因注释, SSR

Abstract: Bolboschoenus planiculmis is a typical rice paddy weed.This research aims to supplement the transcriptome information of this species. Based on high-throughput sequencing technology, we acquired and analyzed the transcriptome of B. planiculmis on Illumina Solexa HiSeq 2000 platform, using a compounded sample of stem, leave, rhizome and tuber. In total, 59 788 Unigene with an average length of 842 bp and N50 of 1 402 bp were obtained by de novo assembly. Aquired Unigene sequences were compared with five main gene data base(NR, Swiss-Prot, KEGG, GO, KOG). 35 221 Unigene have been annotated, 58.91% of the total. 9 698 SSR site were obtained from 12 823 Unigene, of which 2 base and 3 base motif compose 95.93% of total amount. According to KEGG analysis, 13 141 Unigenes were involved in 291 metabolic pathways. 60 Unigene were found to be functional in starch synthesis while 14 Unigene were found to related to rhizome elongation. The gibberellin related genes, together with ribosome pathway, reflect the expanding trend of the below ground rhizome system and the urgent demands of energy. The genes involved in starch synthesis indicate that nutrients deposition begins at the early stage of the expansion. These findings provide basis for further research on the regulating mechanism of below ground network, or provide reference for practices such as weed management and wetland protection.

Key words: Bolboschoenus planiculmis, High-throughput sequencing, Transcriptome, Gene annotation, SSR

中图分类号:

Q78
S54.03

宁宇, 郑彦超, 武高洁, 王义飞, 张曼胤. 扁秆荆三棱不同组织混合样品的转录组分析[J]. 华北农学报, 2019, 34(6): 74-81. doi: 10.7668/hbnxb.201751606.

NING Yu, ZHENG Yanchao, WU Gaojie, WANG Yifei, ZHANG Manyin. Transcriptome Analysis of Bolboschoenus planiculmis Using Mixed Samples from Different Tissues[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 74-81. doi: 10.7668/hbnxb.201751606.

http://www.hbnxb.net/CN/Y2019/V34/I6/74

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