摘要： 利用聚丙烯酰胺凝胶垂直板电泳技术对18个冬小麦品种4个发育期的POD同工酶进行了研究，发现在不同发育时期冬小麦根和叶POD同工酶酶谱均有不同程度的变化。返青期根POD同工酶谱变化较明显，在A区增加1条RA1酶带，在C区增加1条RC1酶带，而其他发育期无这2条酶带或只有痕迹带；拔节期叶POD同工酶谱C区酶带比其他发育期多1条LC1酶带；同时发现根RC4酶带、叶LC5酶带在4个发育期均较为稳定，表现为一级带；A区的RA2酶带也较为稳定，在不同发育期均表现为二级带；冬小麦18个品种间POD同工酶谱无明显差异。

关键词: 冬小麦, 过氧化物酶, 同工酶, 发育变化

Abstract: POD isoenzymes of 18 varieties of winter wheat were studied by using PAGE.The result showed that the zymogram of POD isoenzymes of roots and leaves of winter wheat varied in different degree.Between returning green stage and jointing stage the zymograms of POD isoenzyme of roots varied instinctively.There increased a band RA 1 in region A and a band RC 1 in region C respectively.The two bands didn't appear in other development stages.In region C the zymogram of POD isoenzymes of leaves of jointing stage increased one band LC 1,which didn′t appear in other development stages.It was found that the band RC 4 of roots and band LC 5 of leaves were stable in four development stages,and they appeared as first grade bands.The band RA 2 of roots,in region A,was stable too,and appeared as second grade band zymogram of POD isoenzymes had no significant difference among 18 varieties of winter wheats.

Key words: Winter wheat, Peroxidase, Isoenzyme, Developmental change

中图分类号: 

• S512.1+1