摘要： 利用RAPD分子标记方法对我国46个骨干玉米自交系进行了鉴定研究，结果表明，仅用A6，C6，D2，F₁0，H19，N19等6个引物的指纹组合即可将这46个自交系相互区分开来，不需要针对每个自交系筛选特异性标记，克服了利用特异性标记鉴定玉米自交系的局限性；研究出基于WINDOWS98操作平台的玉米DNA指纹数据库和指纹分析软件。并且该数据库和分析软件对自交系的数量和引物数量都是开放性的，如果要区分开m个自交系，最多只用n个引物即可，二者的关系是：ⁿ≥m。

关键词: 引物组合法, 玉米, DNA指纹, 鉴定

Abstract: Forty-six important inbred lines widely used in China were identified by molecular marker. It is needless to select special molecular marker for each inbred. They were distinguished from each other just by the combination of amplification products of six primers A6,C6,D2,F₁0,H19 and N19. It resolved the limitation of identification of maize inbred lines using special markers. The database of DNA fingerprint of each inbred was established, and made out the software of DNA fingerprint analysis for Windows 98. With the increase of the inbred number, the software will play more and more impotrant role in the inbred identification works. If the number of inbred lines and primers is respectively m and n, the relationship between m and n is 2ⁿ≥m.

Key words: Primer combination, Maize, DNA fingerprint, Identification

中图分类号: 

• S513.033