链霉菌TOR3209挥发性有机化合物对烟草生长发育的影响

doi:10.7668/hbnxb.20196009

摘要/Abstract

摘要：

旨在探讨链霉菌TOR3209产生的挥发性有机化合物(VOCs)促进烟草生长发育作用的生物学机制,为开发高效环保的微生物促生产品及开展应用评价提供理论依据。采用二分隔平皿,鉴定菌株发酵液的VOCs的促生特性,并用固相微萃取及气相色谱-质谱联用技术分析VOCs的组分。链霉菌TOR3209在不同浓度下释放的挥发性有机化合物均能促进烟草植株的生长,其中添加5 mL(6×10⁷ CFU/mL)菌株发酵液的处理组效果最显著,与对照组相比增加238%。从链霉菌TOR3209发酵液的VOCs中共提取32种物质,对其中的己二酸二正丁酯物质进行促生功能验证,结果表明,不同质量浓度的己二酸二正丁酯均对烟草的生长均有促进作用,其中质量浓度为1 mol/mL的促生效果最显著。通过转录组分析发现,处理组的烟草与对照组相比上调了漆酶等多种基因。链霉菌TOR3209的VOCs对烟草具有显著的促生作用,己二酸二正丁酯调控了烟草多个促生相关基因。

关键词: 挥发性有机化合物, 链霉菌, 转录组, 烟草

Abstract:

The biological mechanism of Streptomyces TOR3209 volatile organic compounds(VOCs)promoting tobacco growth and development was studied.The growth-promoting properties of VOCs in the fermentation broth were identified by two separate plates,and the VOCs components were analyzed by solid phase microextraction and gas chromatography-mass spectrometry.VOCs of Streptomyces TOR3209 at different concentrations could promote the growth of tobacco,and the effect of adding 5 mL(6×10⁷ CFU/mL)strain fermentation liquid was the most significant in the treatment group,which increased 238% compared with the control group.A total of 32 substances were extracted from the VOCs of Streptomyces TOR3209 fermentation broth,and the growth-promoting function of dinbutyl adipate was verified.The results showed that dinbutyl adipate of different mass concentrations could promote the growth of tobacco,and the growth promoting effect of 1 mol/mL was the most significant.Through transcriptome analysis,it was found that many genes such as laccase were up-regulated in the tobacco treated group compared with the control group.VOCs of Streptomyces TOR3209 had a significant growth promoting effect on tobacco,and dinbutyl adipate regulated several growth promoting genes in tobacco.This study clarified the mechanism of the VOCs of Streptomyces TOR3209 regulating tobacco growth and development,and provided a theoretical basis for the development of efficient and environmentally friendly microbial production promotion products and the application evaluation.

Key words: Volatile organic compounds, Streptomyces, Transcriptome, Tobacco

中图分类号:

S572
Q946

张梦一, 贺羽茜, 彭杰丽, 赵琳琳, 张秀敏, 杨太新, 胡栋. 链霉菌TOR3209挥发性有机化合物对烟草生长发育的影响[J]. 华北农学报, 2025, 40(S1): 277-284. doi: 10.7668/hbnxb.20196009.

ZHANG Mengyi, HE Yuxi, PENG Jieli, ZHAO Linlin, ZHANG Xiumin, YANG Taixin, HU Dong. Effects of Streptomyces TOR3209 Fermentation Liquid VOCs on Tobacco Growth and Development[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 277-284. doi: 10.7668/hbnxb.20196009.

http://www.hbnxb.net/CN/Y2025/V40/IS1/277

图/表 8

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序号 Number	化合物名称 Name of compound	CAS 编号 CAS number	相对含量/% Relative content
1	己二酸二丁酯	000105-99-7	15.98
2	棕榈酸	000057-10-3	11.49
3	2,4-二叔丁基酚	000096-76-4	5.41
4	十五烷酸	001002-84-2	4.95
5	六噻烷	013798-23-7	3.82
6	1 19-二十碳二烯	014811-95-1	3.07
7	肉豆蔻酸	000544-63-8	2.61
8	(Z)-9-十六碳烯醛	056219-04-6	2.18
9	十七烷酸	000506-12-7	2.30
10	α-panasinsanene	056633-28-4	1.27
11	十六醛	000629-80-1	1.05
12	月桂酸	000143-07-7	0.26
13	二十四烷醇	000506-51-4	0.43
14	正十七烷	000629-78-7	0.82
15	9-Eicosene, (9E)-	074685-29-3	0.92
16	环十五烷	000295-48-7	0.38
17	植酮	000502-69-2	0.49
18	环十四烷	000295-17-0	0.47
19	2,4,6-Trimethyl-undecanoic	055955-76-5	0.59
20	2-十四酮	002345-27-9	0.47
21	α-eudesmol	000473-16-5	0.45
22	十七烷酮	002922-51-2	0.29
23	正十七烷	000629-78-7	1.05
24	2,2,4,4-tetramethyl	097455-13-5	0.27
25	4-氨基-3-甲基苯酚	002835-99-6	0.26
26	2-羟基环十五烷酮	004727-18-8	0.33
27	十六酸乙酯	000628-97-7	0.61
28	十三酸乙酯	028267-29-0	0.47
29	环十六烷	000295-65-8	0.57
30	(3E)-3-十八碳烯	007206-19-1	0.29
31	2-环己烯-1-醇	000822-67-3	0.39
32	十七烷酸乙酯	014010-23-2	0.26

序号 Number	化合物名称 Name of compound	CAS 编号 CAS number	相对含量/% Relative content
1	己二酸二丁酯	000105-99-7	15.98
2	棕榈酸	000057-10-3	11.49
3	2,4-二叔丁基酚	000096-76-4	5.41
4	十五烷酸	001002-84-2	4.95
5	六噻烷	013798-23-7	3.82
6	1 19-二十碳二烯	014811-95-1	3.07
7	肉豆蔻酸	000544-63-8	2.61
8	(Z)-9-十六碳烯醛	056219-04-6	2.18
9	十七烷酸	000506-12-7	2.30
10	α-panasinsanene	056633-28-4	1.27
11	十六醛	000629-80-1	1.05
12	月桂酸	000143-07-7	0.26
13	二十四烷醇	000506-51-4	0.43
14	正十七烷	000629-78-7	0.82
15	9-Eicosene, (9E)-	074685-29-3	0.92
16	环十五烷	000295-48-7	0.38
17	植酮	000502-69-2	0.49
18	环十四烷	000295-17-0	0.47
19	2,4,6-Trimethyl-undecanoic	055955-76-5	0.59
20	2-十四酮	002345-27-9	0.47
21	α-eudesmol	000473-16-5	0.45
22	十七烷酮	002922-51-2	0.29
23	正十七烷	000629-78-7	1.05
24	2,2,4,4-tetramethyl	097455-13-5	0.27
25	4-氨基-3-甲基苯酚	002835-99-6	0.26
26	2-羟基环十五烷酮	004727-18-8	0.33
27	十六酸乙酯	000628-97-7	0.61
28	十三酸乙酯	028267-29-0	0.47
29	环十六烷	000295-65-8	0.57
30	(3E)-3-十八碳烯	007206-19-1	0.29
31	2-环己烯-1-醇	000822-67-3	0.39
32	十七烷酸乙酯	014010-23-2	0.26

己二酸二正丁酯 Dibutyl adipate	鲜质量/g Fresh weight
CK	0.021±0.005b
M1	0.028±0.010b
M2	0.022±0.015b
M3	0.049±0.022a

己二酸二正丁酯 Dibutyl adipate	鲜质量/g Fresh weight
CK	0.021±0.005b
M1	0.028±0.010b
M2	0.022±0.015b
M3	0.049±0.022a

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