基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制

doi:10.7668/hbnxb.20195964

华北农学报 ›› 2025, Vol. 40 ›› Issue (6): 40-49. doi: 10.7668/hbnxb.20195964

所属专题： 盐碱胁迫；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制

刘世森¹^,², 杨以诚³, 冯世纪², 郭珍珠², 张述伟², 郭桂梅², 王雨², 周龙华², 刘成洪², 陈志伟²

¹ 上海海洋大学水产与生命学院,上海 201306
² 上海市农业科学院生物技术研究所, 上海市农业遗传育种重点实验室,上海 201106
³ 上海科学技术交流中心,上海 200235

收稿日期:2025-03-28 出版日期:2025-12-31
通讯作者:
陈志伟(1980—),男,江苏溧阳人,研究员,硕士,硕士生导师,主要从事大麦耐盐性分子机理研究与单倍体育种研究。
作者简介:
刘世森(1998—),男,河南新乡人,在读硕士,主要从事大麦耐盐性鉴定与分子机理研究。
刘世森、杨以诚为同等贡献作者。
基金资助:
农业农村部国家大麦青稞产业技术体系(CARS-05-01A-02); 西藏自治区科技厅西藏自治区科技计划项目(XZ202501ZY0029); 上海市农业农村委上海市科技兴农项目(2019-02-08-00-08-F01109); 上海市农科院卓越团队建设项目(沪农科卓(2022)018)

Molecular Mechanisms of Salt Tolerance in Naked Barley at Seedling Stage Based on Integrated Transcriptomic and Metabolomic Analysis

LIU Shisen¹^,², YANG Yicheng³, FENG Shiji², GUO Zhenzhu², ZHANG Shuwei², GUO Guimei², WANG Yu², ZHOU Longhua², LIU Chenghong², CHEN Zhiwei²

¹ College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China
² Biotechnology Research Institute,Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences,Shanghai 201106,China
³ Shanghai Science and Technology Exchange Center,Shanghai 200235,China

Received:2025-03-28 Published:2025-12-31

摘要/Abstract

摘要： 盐胁迫对作物的产量和品质造成巨大的威胁,而大麦作为耐盐研究的先锋作物之一,开展其耐盐机理的解析,可为作物耐盐育种提供理论依据。以前期获得的盐敏感裸大麦地方品种B87和耐盐裸大麦地方品种B94为供试材料,在三叶期使用200 mmol/L NaCl处理7 d后,对其地上部组织进行了转录组和代谢组测序,并通过联合分析揭示裸大麦的耐盐性分子机理。结果显示,转录组和代谢组分析中,B87鉴定到了2 240个差异表达基因(DEGs)和198个差异丰度代谢物(DAMs),而B94鉴定到了923个DEGs和232个DAMs。经韦恩分析,耐盐裸大麦品种B94中特异DEGs和特异DAMs分别为480,129个。进而,又对DEGs和DAMs分别进行了GO和KEGG分析,其中B94的DEGs特异显著富集到11个通路,而其DAMs则仅特异显著富集到1个通路。另外,还对转录组和代谢组进行了相关性分析,发现基因与代谢物的变化既存在一致性,也存在不一致性。这些工作不仅丰富了对裸大麦耐盐性分子机理的理解,也为未来裸大麦耐盐育种提供了有价值的线索。

关键词: 裸大麦, 耐盐性, 转录组, 代谢组, 定量PCR

Abstract:

Salt stress causes a significant threat to crop yield and quality.As one of the pioneer crop species in salt tolerance research,barley holds critical significance;the exploration of its salt tolerance mechanisms is capable of providing a theoretical foundation for crop salt-tolerance breeding programs.Two naked barley landraces,namely B87 with salt-sensitivity and B94 with salt-tolerance,were employed as experimental materials.At the three-leaf stage,their seedlings were exposed to a 200 mmol/L NaCl treatment for 7 days.Subsequent to the treatment,the above-ground tissues were collected for transcriptomic and metabolomic sequencing.By means of integrated multi-omics analysis,this study was designed to elucidate the molecular mechanisms governing salt tolerance in naked barley.The results demonstrated that 2 240 differentially expressed genes (DEGs) and 198 differentially abundant metabolites (DAMs) were identified in B87 via transcriptomic and metabolomic profiling,whereas 923 DEGs and 232 DAMs were detected in B94.Venn diagram analysis further revealed that the salt-tolerant naked barley B94 contained 480 specific DEGs and 129 specific DAMs.Furthermore, GO and KEGG analyses were separately performed on the DEGs and DAMs. And the DEGs of B94 were significantly enriched in 11 unique pathways, while its DAMs were only significantly enriched in 1 unique pathway. In addition, correlation analysis between the transcriptome and metabolome was conducted, and it was found that the changes in genes and metabolites exhibited both consistency and inconsistency. These research efforts not only enhance the current understanding of the molecular mechanisms underlying salt tolerance in naked barley,but also provide valuable insights and candidate targets for the development of salt-tolerant naked barley cultivars in future breeding.

Key words: Naked barley, Salt tolerance, Transcriptome, Metabolome, qPCR

中图分类号:

S512.3大麦

刘世森, 杨以诚, 冯世纪, 郭珍珠, 张述伟, 郭桂梅, 王雨, 周龙华, 刘成洪, 陈志伟. 基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制[J]. 华北农学报, 2025, 40(6): 40-49. doi: 10.7668/hbnxb.20195964.

LIU Shisen, YANG Yicheng, FENG Shiji, GUO Zhenzhu, ZHANG Shuwei, GUO Guimei, WANG Yu, ZHOU Longhua, LIU Chenghong, CHEN Zhiwei. Molecular Mechanisms of Salt Tolerance in Naked Barley at Seedling Stage Based on Integrated Transcriptomic and Metabolomic Analysis[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 40-49. doi: 10.7668/hbnxb.20195964.

http://www.hbnxb.net/CN/Y2025/V40/I6/40

图/表 9

表1 对照(CK)和盐处理(ST)条件下裸大麦幼苗地上部的RNA-seq数据质量

Tab.1 Quality of RNA-seq data of shoots of naked barley seedlings grown under control (CK) and salt treatment (ST)

样本 Sample	原始碱基数/ Gb Raw bases	有效碱基数/ Gb Clean bases	Q30/ %	GC含量/ % GC content
CK87_1	7.03	6.94	95.50	56.87
CK87_2	7.15	7.04	95.55	56.71
CK87_3	7.09	7.02	95.30	56.96
CK94_1	7.06	6.96	95.36	57.21
CK94_2	6.99	6.91	95.49	56.99
CK94_3	7.07	7.01	95.71	56.50
ST87_1	7.16	7.04	95.46	55.80
ST87_2	6.97	6.89	95.99	57.02
ST87_3	6.96	6.88	95.76	56.23
ST94_1	7.01	6.92	95.71	56.53
ST94_2	6.96	6.89	95.46	56.20
ST94_3	7.00	6.94	95.66	57.17

表2 qPCR分析的引物

Tab.2 Primers used for qPCR analysis

基因名称 Gene name	引物序列(5'—3') Primer sequences(5'—3')	扩增长度/bp Amplicon	扩增效率 PCR efficiency	引物来源 Primer origin
HORVU2Hr1G029900	F:CAACCGTAGGTACTCCTGCC	119	1.867 2	本研究
	R:AGCACCGACAAGCATCACAT
HORVU7Hr1G090410	F:CGCGACGACATCTCCATCTT	269	1.810 3
	R:TTCCCGGACTTGTCAGAACG
HORVU6Hr1G000510	F:GCTGCACGGATTATGCACTG	241	1.812 1
	R:CTCGGGGGTTTCGAACTTGA
HORVU5Hr1G010130	F:TGCAGATCGCTCTCTTCGTC	105	1.860 4
	R:AATCAGGGCGGTTAGTGTCG
HORVU2Hr1G110230	F:GATGGTAGTGTCGCTCCTCG	117	1.873 6
	R:GACCTCCGGAGCGATGTATG
HORVU3Hr1G029350	F:AGGATTCTCCAGTTTGCTGCT	199	1.803 7
	R:ACACGCAACACCTTCTCCTT
HORVU1Hr1G093780	F:TCTGCTCGGGCCTCAAGTT	133	1.833 4
	R:TCGATGTGCTCCGTGTTGTA
U61	F:GAGGAAACGAAACCTGTGC	65	1.828 7	[17]
	R:ACTTCTTAGAGGGTTGTGTTAC
snoR14	F:GATGTTTATGTATGATAGTCTGTC	67	1.811 8
	R:GTCGGGATGTATGCGTGTC

图1 盐胁迫下B87和B94幼苗地上部差异表达基因分析 A.B87差异表达基因聚类分析;B.B94差异表达基因聚类分析;C.不同品种差异表达基因数量的柱状图;D.B87与B94间差异表达基因韦恩图。

Fig.1 Analysis of differentially expressed genes (DEGs) in shoots of B87 and B94 seedlings under salt stress A.Cluster analysis of DEGs in B87;B.Cluster analysis of DEGs in B94;C.The histogram of DEGs in B87 and B94,respectively;D.Venn diagram of DEGs between B87 and B94.

图2 B87和B94差异表达基因的GO富集分析 A.B87的上调基因富集GO条目:1.防卫反应,2.核糖体RNA加工,3.对生物刺激的反应,4.药物跨膜转运,5.氧脂素生物合成过程,6.对水的反应,7.RNA分解代谢过程,8.snRNA假尿嘧啶核苷合成,9.种子发芽的正向调节,10.小亚基核糖体RNA成熟,11.蛋白复合物寡聚化,12.蜀黍苷生物合成过程,13.核糖体RNA假尿嘧啶核苷合成,14.L-天冬酰胺生物合成过程,15.mRNA假尿嘧啶核苷合成,16.精氨酸分解代谢过程,17.叶绿体膜,18.小亚基加工体,19.H/ACA盒snoRNP复合物,20.小核仁核糖核蛋白复合物,21.铁离子结合,22.毒素活性,23.snoRNA 结合,24.核糖核酸内切酶活性,25.亚油酸酯13S-脂氧合酶活性,26.核糖核酸酶T2活性,27.几丁质酶活性,28.核糖体RNA N-糖基化酶活性,29.天冬酰胺合酶(谷氨酰胺水解型)活性,30.镉离子跨膜转运酶活性;B.B94的上调基因富集GO条目:1.防卫反应,2.对镉离子反应,3.对真菌的防卫反应,4.碳水化合物代谢过程,5.对生物刺激的反应,6.脂质运输,7.对损伤的反应,8.氧脂素生物合成过程,9.RNA聚合酶Ⅱ对转录的调控,10.对含砷物质的反应,11.对一氧化氮的反应,12.对水的反应,13.乙烯激活信号通路的调控,14.对铁离子饥饿的反应,15.杀死其他生物的细胞,16.含花青素的化合物生物合成过程,17.种子发芽的正向调节,18.L-哌啶酸生物合成过程,19.胞外区,20.毒素活性,21.单加氧酶活性,22.脂质结合,23.葡聚糖内切-1,3-β-D-葡萄糖苷酶活性,24.东莨菪苷β-葡萄糖苷酶活性,25.β-葡萄糖苷酶活性,26.丝氨酸型肽链内切酶抑制剂活性,27.亚油酸酯 13S-脂氧合酶活性,28.蛋白羧基端结合,29.核糖核酸酶T2活性,30.蔗糖合酶活性;C.B87的下调基因富集GO条目:1.对氧化胁迫的反应,2.过氧化氢分解代谢过程,3.光合作用、光能捕获,4.细胞壁生成过程,5.木聚糖代谢过程,6.基于微管的过程,7.锌离子跨膜转运,8.角质生物合成过程,9.胞间连丝介导的细胞间运输,10.寡肽转运,11.阿拉伯糖分解代谢过程,12.质膜,13.胞外区,14.细胞壁,15.胞间连丝,16.质外体,17.植物型细胞壁,18.膜锚定成分,19.质膜锚定成分,20.DNA结合转录因子活性,21.过氧化物酶活性,22.锰离子结合,23.营养储存库活动,24.木葡聚糖:木糖基转移酶活性,25.细胞骨架的结构成分,26.水解酶活性,水解 O-糖基化合物,27.原叶绿素酸酯还原酶活性,28.锌离子跨膜转运活性,29.硝酸盐跨膜转运酶活性,30.α-L-阿拉伯呋喃糖苷酶活性;D.B94的下调基因富集GO条目:1.细胞壁结构,2.核小体组装,3.过氧化氢分解代谢过程,4.脂质分解代谢过程,5.木质素分解代谢过程,6.果实成熟,7.乙烯生物合成过程,8.寡肽转运,9.胞外区,10.核小体,11.细胞壁,12.质外体,13.植物型细胞壁,14.DNA 结合,15.蛋白异二聚化活性,16.血红素结合,17.过氧化物酶活性,18.铜离子结合,19.氧化还原酶活性、氧化金属离子,20.氢醌:氧气氧化还原酶活性,21.3-氧代花生酰辅酶A合成酶活性,22.3-氧代花生四烯酰辅酶A合成酶活性,23.3-氧代木糖基辅酶A合成酶活性,24.超长链3-酮酰辅酶A合成酶活性,25.细胞壁结构成分,26.葡聚糖外1,3-β-葡萄糖苷酶活性,27.蔗糖α-葡萄糖苷酶活性,28.硝酸盐跨膜转运活性,29.蔗糖 1F-果糖基转移酶活性,30.天冬酰胺酶活性。

Fig.2 GO enrichment analysis of differentially expressed genes (DEGs) in B87 and B94 A.Enriched GO terms of up-regulated genes in B87:1.Defense response,2.rRNA processing,3.Response to biotic stimulus,4.Drug transmembrane transport,5.Oxylipin biosynthetic process,6.Response to water,7.RNA catabolic process,8.snRNA pseudouridine synthesis,9.Positive regulation of seed germination,10.Maturation of SSU-rRNA,11.Protein complex oligomerization,12.Dhurrin biosynthetic process,13.rRNA pseudouridine synthesis,14.L-asparagine biosynthetic process,15.mRNA pseudouridine synthesis,16.Arginine catabolic process,17.Chloroplast membrane,18.Small-subunit processome,19.Box H/ACA snoRNP complex,20.Small nucleolar ribonucleoprotein complex,21.Iron ion binding,22.Toxin activity,23.snoRNA binding,24.Endoribonuclease activity,25.Linoleate 13S-lipoxygenase activity,26.Ribonuclease T2 activity,27.Chitinase activity,28.rRNA N-glycosylase activity,29.Asparagine synthase (glutamine-hydrolyzing) activity,30.Cadmium ion transmembrane transporter activity;B.Enriched GO terms of up-regulated genes in B94:1.Defense response,2.Response to cadmium ion,3.Defense response to fungus,4.Carbohydrate metabolic process,5.Response to biotic stimulus,6.Lipid transport,7.Response to wounding,8.Oxylipin biosynthetic process,9.Regulation of transcription by RNA polymerase Ⅱ,10.Response to arsenic-containing substance,11.Response to nitric oxide,12.Response to water,13.Regulation of ethylene-activated signaling pathway,14.Response to iron ion starvation,15.Killing of cells of other organism,16.Anthocyanin-containing compound biosynthetic process,17.Positive regulation of seed germination,18.L-pipecolic acid biosynthetic process,19.Extracellular region,20.Toxin activity,21.Monooxygenase activity,22.Lipid binding,23.Glucan endo-1,3-beta-D-glucosidase activity,24.Scopolin beta-glucosidase activity,25.Beta-glucosidase activity,26.Serine-type endopeptidase inhibitor activity,27.Linoleate 13S-lipoxygenase activity,28.Protein C-terminus binding,29.Ribonuclease T2 activity,30.Sucrose synthase activity;C.Enriched GO terms of down-regulated genes in B87:1.Response to oxidative stress,2.Hydrogen peroxide catabolic process,3.Photosynthesis,light harvesting,4.Cell wall biogenesis,5.Xyloglucan metabolic process,6.Microtubule-based process,7.Zinc ion transmembrane transport,8.Cutin biosynthetic process,9.Plasmodesmata-mediated intercellular transport,10.Oligopeptide transport,11.Arabinan catabolic process,12.Plasma membrane,13.Extracellular region,14.Cell wall,15.Plasmodesma,16.Apoplast,17.Plant-type cell wall,18.Anchored component of membrane,19.Anchored component of plasma membrane,20.DNA-binding transcription factor activity,21.Peroxidase activity,22.Manganese ion binding,23.Nutrient reservoir activity,24.Xyloglucan:xyloglucosyl transferase activity,25.Structural constituent of cytoskeleton,26.Hydrolase activity,hydrolyzing O-glycosyl compounds,27.Protochlorophyllide reductase activity,28.Zinc ion transmembrane transporter activity,29.Nitrate transmembrane transporter activity,30.Alpha-L-arabinofuranosidase activity;D.Enriched GO terms of down-regulated genes in B94:1.Cell wall organization,2.Nucleosome assembly,3.Hydrogen peroxide catabolic process,4.Lipid catabolic process,5.Lignin catabolic process,6.Fruit ripening,7.Ethylene biosynthetic process,8.Oligopeptide transport,9.Extracellular region,10.Nucleosome,11.Cell wall,12.Apoplast,13.Plant-type cell wall,14.DNA binding,15.Protein heterodimerization activity,16.Heme binding,17.Peroxidase activity,18.Copper ion binding,19.Oxidoreductase activity,oxidizing metal ions,20.Hydroquinone:oxygen oxidoreductase activity,21.3-oxo-arachidoyl-CoA synthase activity,22.3-oxo-cerotoyl-CoA synthase activity,23.3-oxo-lignoceronyl-CoA synthase activity,24.Very-long-chain 3-ketoacyl-CoA synthase activity,25.Structural constituent of cell wall,26.Glucan exo-1,3-beta-glucosidase activity,27.Sucrose alpha-glucosidase activity,28.Nitrate transmembrane transporter activity,29.Sucrose 1F-fructosyltransferase activity,30.Asparaginase activity.

图3 B87和B94差异表达基因的KEEG富集分析 A.B87上调基因富集KEGG通路:1.真核生物核糖体生物合成,2.亚麻酸代谢,3.丙氨酸、天冬氨酸和谷氨酸代谢,4.氰基氨基酸代谢,5.芥子油苷生物合成,6.异喹啉生物碱生物合成,7.精氨酸和脯氨酸代谢,8.ABC转运体,9.牛磺酸和低牛磺酸代谢,10.β-丙氨酸代谢,11.酪氨酸代谢,12.丁酸代谢,13.MAPK信号通路-植物,14.苯丙氨酸代谢,15.半胱氨酸和蛋氨酸代谢,16.RNA降解,17.甘氨酸、丝氨酸和苏氨酸代谢,18.托品烷、哌啶和吡啶生物碱生物合成,19.谷胱甘肽代谢,20.淀粉和蔗糖代谢,21.吲哚生物碱生物合成,22.黄酮和黄酮醇生物合成;B.B94上调基因富集KEGG通路:1.亚麻酸代谢,2.氰氨基酸代谢,3.MAPK信号通路-植物,4.植物激素信号转导,5.角质、栓质和蜡质生物合成,6.黄酮和黄酮醇生物合成,7.芥子油苷生物合成,8.淀粉和蔗糖代谢,9.其他糖苷降解,10.丙氨酸、天冬氨酸和谷氨酸代谢,11.丁酸代谢;C.B87下调基因富集KEGG通路:1.苯丙烷生物合成,2.光合作用-天线蛋白,3.植物-病原体相互作用,4.卟啉和叶绿素代谢,5.吞噬体,6.二萜生物合成,7.MAPK信号通路-植物,8.糖胺聚糖降解,9.角质、栓质和蜡质生物合成,10.氮代谢,11.吲哚生物碱生物合成,12.氨基糖和核苷酸糖代谢,13.甘油磷脂代谢;D.B94下调基因富集KEGG通路:1.脂肪酸延长,2.苯丙烷生物合成,3.氮代谢,4.植物-病原体相互作用,5.二萜生物合成,6.半萜和三萜生物合成,7.半胱氨酸和蛋氨酸代谢,8.吲哚生物碱生物合成,9.其他聚糖生物合成,10.丙氨酸、天冬氨酸和谷氨酸代谢,11.植物激素信号转导,12.氰基氨基酸代谢,13.不饱和脂肪酸生物合成,14.MAPK信号通路-植物,15.生长素生物合成。

Fig.3 KEGG enrichment analysis of differentially expressed genes (DEGs) in B87 and B94 A.Enriched KEGG pathways of up-regulated gene in B87:1.Ribosome biogenesis in eukaryotes,2.Linoleic acid metabolism,3.Alanine,aspartate and glutamate metabolism,4.Cyanoamino acid metabolism,5.Glucosinolate biosynthesis,6.Isoquinoline alkaloid biosynthesis,7.Arginine and proline metabolism,8.ABC transporters,9.Taurine and hypotaurine metabolism,10.beta-Alanine metabolism,11.Tyrosine metabolism,12.Butanoate metabolism,13.MAPK signaling pathway-plant,14.Phenylalanine metabolism,15.Cysteine and methionine metabolism,16.RNA degradation,17.Glycine,serine and threonine metabolism,18.Tropane,piperidine and pyridine alkaloid biosynthesis,19.Glutathione metabolism,20.Starch and sucrose metabolism,21.Indole alkaloid biosynthesis,22.Flavone and flavonol biosynthesis;B.Enriched KEGG pathways of up-regulated genes in B94:1.Linoleic acid metabolism,2.Cyanoamino acid metabolism,3.MAPK signaling pathway-plant,4.Plant hormone signal transduction,5.Cutin,suberine and wax biosynthesis,6.Flavone and flavonol biosynthesis,7.Glucosinolate biosynthesis,8.Starch and sucrose metabolism,9.Other glycan degradation,10.Alanine,aspartate and glutamate metabolism,11.Butanoate metabolism;C.Enriched KEGG pathways of down-regulated genes in B87:1.Phenylpropanoid biosynthesis,2.Photosynthesis-antenna proteins,3.Plant-pathogen interaction,4.Porphyrin and chlorophyll metabolism,5.Phagosome,6.Diterpenoid biosynthesis,7.MAPK signaling pathway-plant,8.Glycosaminoglycan degradation,9.Cutin,suberine and wax biosynthesis,10.Nitrogen metabolism,11.Indole alkaloid biosynthesis,12.Amino sugar and nucleotide sugar metabolism,13.Glycerophospholipid metabolism;D.Enriched KEGG pathways of down-regulated genes in B94:1.Fatty acid elongation,2.Phenylpropanoid biosynthesis,3.Nitrogen metabolism,4.Plant-pathogen interaction,5.Diterpenoid biosynthesis,6.Sesquiterpenoid and triterpenoid biosynthesis,7.Cysteine and methionine metabolism,8.Indole alkaloid biosynthesis,9.Betalain biosynthesis,10.Alanine,aspartate and glutamate metabolism,11.Plant hormone signal transduction,12.Cyanoamino acid metabolism,13.Biosynthesis of unsaturated fatty acids,14.MAPK signaling pathway-plant,15.Brassinosteroid biosynthesis.

图4 盐胁迫下B87和B94幼苗地上部差异丰度代谢物分析 A.所有样品代谢物的主成分分析;B.差异丰度代谢物数量柱状图;C.B87与B94间上调代谢物韦恩图;D.B87与B94间下调代谢物韦恩图。

Fig.4 Analysis of differentially abundant metabolites (DAMs) in shoots of B87 and B94 seedlings under salt stress A.Principal component analysis (PCA) of metabolites in all samples;B.The histogram of DAMs;C.Venn diagram of up-regulated abundant metabolites between B87 and B94;D.Venn diagram of down-regulated abundant metabolites between B87 and B94.

图5 B87和B94差异丰度代谢物KEEG富集分析 A.B87上调代谢物富集通路:1.色氨酸代谢,2.D-氨基酸代谢,3.甘氨酸、丝氨酸和苏氨酸代谢,4.酪氨酸代谢;B.B94上调代谢物富集通路:1.α-亚麻酸代谢,2.甘氨酸、丝氨酸和苏氨酸代谢,3.色氨酸代谢;C.B87下调代谢物富集通路:1.光合生物碳固定,2.丙氨酸、天冬氨酸和谷氨酸代谢,3.氰基氨基酸代谢,4.半乳糖代谢,5.氨酰-tRNA生物合成,6.甘油磷脂代谢,7.D-氨基酸代谢,8.叶酸介导的一碳单位库;D.B94下调代谢物富集通路:1.丙氨酸、天冬氨酸和谷氨酸代谢。

Fig.5 KEGG enrichment analysis of differentially abundant metabolites (DAMs) in B87 and B94 A.Enriched pathways of up-regulated metabolites in B87:1.Tryptophan metabolism,2.D-Amino acid metabolism,3.Glycine,serine and threonine metabolism,4.Tyrosine metabolism;B.Enriched pathways of up-regulated metabolites in B94:1.Alpha-Linolenic acid metabolism,2.Glycine,serine and threonine metabolism,3.Tryptophan metabolism;C.Enriched pathways of down-regulated metabolites in B94:1.Carbon fixation in photosynthetic organisms,2.Alanine,aspartate and glutamate metabolism,3.Cyanoamino acid metabolism,4.Galactose metabolism,5.Aminoacyl-tRNA biosynthesis,6.Glycerophospholipid metabolism,7.D-Amino acid metabolism,8.One carbon pool by folate;D.Enriched pathways of down-regulated metabolites in B94:1.Alanine,aspartate and glutamate metabolism.

图6 差异表达基因与差异丰度代谢互作的Cytoscape网络图 A.B87;B.B94。圆形代表基因,方形代表代谢物,红线和绿线分别表示正和负相关关系。

Fig.6 Cytoscape network of DEGs and DAMs A.B87;B.B94.Circles represent genes,squares represent metabolites,and red and green lines indicate positive and negative correlations,respectively.

图7 定量PCR验证每个柱状图矩形框上方不同字母,代表对应基因的表达量在对照与处理间存在显著差异(P<0.05)。右下角的热图为对应基因在转录组分析中的情况。

Fig.7 Validation by qPCR Different letters above the rectangular box of each histogram represent that the expression level of the corresponding gene is significantly different between the control(CK) and the salt treatment (ST) (P<0.05).The heatmap in the lower right corner shows their situations in transcriptome analysis.

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基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制

Molecular Mechanisms of Salt Tolerance in Naked Barley at Seedling Stage Based on Integrated Transcriptomic and Metabolomic Analysis

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基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制

Molecular Mechanisms of Salt Tolerance in Naked Barley at Seedling Stage Based on Integrated Transcriptomic and Metabolomic Analysis

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