中国大鲵<i>Galectin-3</i>基因克隆、生物信息学及表达特性分析

doi:10.7668/hbnxb.20195469

华北农学报 ›› 2025, Vol. 40 ›› Issue (5): 231-238. doi: 10.7668/hbnxb.20195469

• 畜牧·水产·兽医 • 上一篇

中国大鲵Galectin-3基因克隆、生物信息学及表达特性分析

商文聪¹^,²^,³^,⁴, 黎琴容¹^,²^,³^,⁴, 吴飞凡¹^,²^,³, 苏淑贤¹^,²^,³, 骆建林¹^,²^,³^,⁴, 王开卓¹^,²^,³^,⁴, 王慧¹^,²^,³^,⁴, 孟永禄¹^,²^,³^,⁴, 叶婷¹^,²^,³^,⁴, 李灿¹^,²^,³^,⁴^,

¹ 贵阳学院, 贵州贵阳 550005
² 贵州省山地生物资源保护和高效利用工程研究中心, 贵州贵阳 550005
³ 贵州省山地珍稀动物与经济昆虫重点实验室, 贵州贵阳 550005
⁴ 贵阳市农业(生态渔业)产业技术创新中心, 贵州贵阳 550005

收稿日期:2024-11-29 出版日期:2025-11-03
通讯作者:
李灿(1979—),男,贵州贵阳人,教授,博士,主要从事山地珍稀动物与经济昆虫研究。
作者简介:
商文聪(1986—),女,山东荣成人,副教授,博士,硕士生导师,主要从事中国大鲵病害免疫及大鲵功能蛋白的开发与应用研究。
基金资助:
贵州省基础研究计划项目(黔科合基础-ZK〔2022〕一般011(黔科合基础-ZK〔2022〕一般011); 贵阳学院博士科研启动基金项目(2019039510819); 贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2020]091); 中央财政引导地方科技发展专项(QKZYD〔2022〕4013); 贵州省科技厅贵州省山地珍稀动物与经济昆虫重点实验室项目(k18200006)

Cloning,Bioinformatics and Expression Characteristics Analysis of Galectin-3 Gene in Chinese Giant Salamander

SHANG Wencong¹^,²^,³^,⁴, LI Qinrong¹^,²^,³^,⁴, WU Feifan¹^,²^,³, SU Shuxian¹^,²^,³, LUO Jianlin¹^,²^,³^,⁴, WANG Kaizhuo¹^,²^,³^,⁴, WANG Hui¹^,²^,³^,⁴, MENG Yonglu¹^,²^,³^,⁴, YE Ting¹^,²^,³^,⁴, LI Can¹^,²^,³^,⁴^,

¹ Guiyang University, Guiyang 550005, China
² Guizhou Provincial Engineering Research Center for Biological Resources Protection and Efficient Utilization of the Mountainous Region, Guiyang 550005, China
³ Guizhou Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang 550005, China
⁴ Guiyang Agricultural(Ecological Fishery) Industry Technology Innovation Center,, Guiyang 550005, China

Received:2024-11-29 Published:2025-11-03

摘要/Abstract

摘要： 为明确中国大鲵Galectin-3基因的分子生物学特征以及染菌前后的表达模式,通过RT-PCR技术对大鲵Galectin-3基因进行了扩增,通过生物信息学技术分析其理化性质和蛋白结构,利用实时荧光定量PCR(qRT-PCR)技术检测了嗜水气单胞菌侵染前后中国大鲵不同组织中Galectin-3的表达情况。结果表明,大鲵Galectin-3基因CDS区长度为351 bp,编码116个氨基酸,理论等电点(pI)为5.87,相对分子质量为13.53 ku,为稳定的亲水蛋白,定位于细胞质;结构分析显示,大鲵Galectin-3蛋白含有一个GLECT结构域;系统发育进化树显示,大鲵Galectin-3氨基酸序列与双条吻蚓的亲缘关系最近。qRT-PCR结果显示,大鲵Galectin-3在所测各组织中均有表达,且在皮肤中的相对表达量最高;在大鲵肝脏、肌肉、肠和肾脏组织中,嗜水气单胞菌感染12 h,Galectin-3的相对表达量显著上升并达到最高值;而在大鲵脾脏和皮肤组织中,嗜水气单胞菌感染48 h,Galectin-3的相对表达量显著上升并达到最高值。综上,在外源菌的侵染下大鲵Galectin-3基因在早期积极响应表达,揭示Galectin-3基因可能参与了大鲵的免疫调控作用。

关键词: 大鲵, Galectin-3, 生物信息学分析, 实时荧光定量PCR, 嗜水气单胞菌

Abstract:

In order to identify the molecular biological characteristics and expression patterns of Galectin-3 gene in Chinese giant salamander before and after infection,the Galectin-3 gene was amplified by RT-PCR,and its physicochemical properties and protein structure were analyzed by bioinformatics.Real-time Quantitative PCR(qRT-PCR)was used to detect the expression of Galectin-3 in different tissues of Chinese giant salamanders before and after Aeromonas hydrophila.The results showed that the length of Galectin-3 gene CDS region was 351 bp,encoding 116 amino acids,the theoretical isoelectric point(pI)was 5.87,the relative molecular weight was 13.53 ku,and it was a stable hydrophilic protein located in the cytoplasm.Structural analysis showed that Galectin-3 protein contained a GLECT domain.The phylogenetic tree showed that the Galectin-3 amino acid sequence was closely related to Rhinatrema bivittatum.qRT-PCR results showed that Galectin-3 was expressed in all tested tissues of the salamander,and the relative expression level was the highest in the skin.The relative expression levels of Galectin-3 in the liver,muscle,intestines and kidneys of Chinese giant salamander increased significantly and reached the highest value at 12 h of infection with A.hydrophila.The relative expression levels of Galectin-3 in the spleen and skin of Chinese giant salamander increased significantly and reached the highest value at 48 h of infection with A.hydrophila.In conclusion,Galectin-3 gene was actively expressed in the early stage of salamander infected by foreign bacteria,suggesting that Galectin-3 gene may be involved in the immune regulation of salamander.

Key words: Andrias davidianus, Galectin-3, Bioinformatics analysis, Real-time Fluorescence Quantitative PCR, Aeromonas hydrophila

中图分类号:

S917水产生物学

商文聪, 黎琴容, 吴飞凡, 苏淑贤, 骆建林, 王开卓, 王慧, 孟永禄, 叶婷, 李灿. 中国大鲵Galectin-3基因克隆、生物信息学及表达特性分析[J]. 华北农学报, 2025, 40(5): 231-238. doi: 10.7668/hbnxb.20195469.

SHANG Wencong, LI Qinrong, WU Feifan, SU Shuxian, LUO Jianlin, WANG Kaizhuo, WANG Hui, MENG Yonglu, YE Ting, LI Can. Cloning,Bioinformatics and Expression Characteristics Analysis of Galectin-3 Gene in Chinese Giant Salamander[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 231-238. doi: 10.7668/hbnxb.20195469.

http://www.hbnxb.net/CN/Y2025/V40/I5/231

图/表 10

表1 引物信息

Tab.1 Primer information

引物名称 Primer names	引物序列(5'—3') Primer sequence(5'—3')	用途 Usage
Galectin-3-RT-F	CTTCTTCATAAAGCACC	Galectin-3片段扩增
Galectin-3-RT-R	TATTCCGCATTGGGTGAC
Galectin-3-CDS-RT-F	TGGCATCAGACCATCAAGAC	Galectin-3 CDS扩增
Galectin-3-CDS-RT-R	TCATAGGATTTTTATATTCAACG
β-actin-F	TGAACCCAAAAGCCAACCGAGAAAAGAT	内参基因
β-actin-R	TACGACCAGAGGCATACAGGGACAGGAC
Galectin-3-Q-F	ACTTGGCTAACGGAGTTGGA	实时荧光定量PCR
Galectin-3-Q-R	AATCCCCAGAGGCGGAAAAC

图1 大鲵Galectin-3基因PCR扩增产物琼脂糖凝胶电泳 M.DL2000 DNA Marker;1.全长扩增产物;2.CDS区扩增产物。

Fig.1 Agarose gel electrophoresis of PCR amplified products of Galectin-3 gene in Andrias davidianus M.DL2000 DNA Marker;1.Full length amplification product; 2.CDS amplification products.

图2 大鲵Galectin-3推导的氨基酸序列大鲵Galectin-3基因编码的氨基酸序列,红色为起始密码子与终止密码子。

Fig.2 Deduced amino acid sequence of the giant salamander Galectin-3 Coded amino acid sequence of A.davidianus Galectin-3 gene with start and stop codons in red.

图3 大鲵Galectin-3编码蛋白的疏/亲水性分析结果

Fig.3 Hydrophobic/hydrophilic analysis of Galectin-3-encoded proteins in the giant salamander

图4 中国大鲵Galectin-3蛋白的保守结构域预测

Fig.4 Prediction of conserved domain of A.davidianus Galectin-3 protein

图5 大鲵Galectin-3氨基酸序列磷酸化位点分析

Fig.5 Phosphorylation site analysis of Galectin-3 amino acid sequence in the giant salamander

图6 大鲵Galectin-3蛋白二级结构预测(A)和三级结构预测(B) A.二级结构预测:黄色.无规卷曲,紫色.延伸链,蓝色.α-螺旋;B.三级结构预测。

Fig 6 Secondary structure prediction(A)and tertiary structure prediction(B)of A.davidianus Galectin-3 protein A.Secondary structure prediction:Yellow.Random coil, Purple.Extended strand,Blue.α-helix;B.Tertiary structure prediction.

图7 Galectin-3蛋白系统进化树

Fig 7 Galectin-3 protein phylogenetic tree

图8 健康大鲵不同组织中Galectin-3的表达水平不同组织间差异显著(P<0.05)用不同的小写字母表示。

Fig.8 Expression levels of Galectin-3 in different tissues of healthy giant salamander Different lowercase letters indicate significant differences between different tissues(P<0.05).

图9 染菌后Galectin-3基因在大鲵不同组织中的表达不同的大写字母代表同一时间点不同处理组之间的差异显著(P<0.05);不同小写字母代表不同时间点同一处理组间差异显著(P<0.05)。

Fig.9 The expression of Galectin-3 in different tissues of A.davidianus after infection Different capital letters indicate significant differences(P<0.05)between different treatment groups at the same time point,and different lowercase letters indicate significant difference(P<0.05)between the same treatment groups at different time points.

参考文献 35

[9]	Soares L C, Al-Dalahmah O, Hillis J, Young C C, Asbed I, Sakaguchi M, O'Neill E, Szele F G. Novel galectin-3 roles in neurogenesis,inflammation and neurological diseases[J]. Cells, 2021, 10(11):3047.doi:10.3390/cells10113047.
[10]	Sygitowicz G, Maciejak-Jastrzębska A, Sitkiewicz D. The diagnostic and therapeutic potential of galectin-3 in cardiovascular diseases[J]. Biomolecules, 2022, 12(1):46.doi:10.3390/biom12010046.
[11]	Blanda V, Bracale U M, Di Taranto M D, Fortunato G. Galectin-3 in cardiovascular diseases[J]. International Journal of Molecular Sciences, 2020, 21(23):9232.doi:10.3390/ijms21239232.
[12]	Bouffette S, Botez I, Targeting galectin-3 in inflammatory and fibrotic diseases[J]. Trends in Pharmacological Sciences, 2023, 44(8):519-531.doi:10.1016/j.tips.2023.06.001.
[13]	Díaz-Alvarez L, Ortega E. The many roles of galectin-3,a multifaceted molecule,in innate immune responses against pathogens[J]. Mediators of Inflammation, 2017, 2017:9247574.doi:10.1155/2017/9247574.
[14]	Yang M L, Chen Y C, Wang C T, Chong H E, Chung N H, Leu C H, Liu F T, Lai M M C, Ling P, Wu C L, Shiau A L. Upregulation of galectin-3 in Influenza A virus infection promotes viral RNA synthesis through its association with viral PA protein[J]. Journal of Biomedical Science, 2023, 30(1):14.doi:10.1186/s12929-023-00901-x.
[15]	Niu J Z, Huang Y, Liu X C, Luo G L, Tang J F, Wang B, Lu Y S, Cai J, Jian J C. Functional characterization of galectin-3 from Nile tilapia(Oreochromis niloticus)and its regulatory role on monocytes/macrophages[J]. Fish & Shellfish Immunology, 2019, 95:268-276.doi:10.1016/j.fsi.2019.10.043.
[16]	Zhu D H, Huang R, Chu P F, Chen L M, Li Y Y, He L B, Li Y M, Liao L J, Zhu Z Y, Wang Y P. Characterization and expression of galectin-3 in grass carp(Ctenopharyngodon idella)[J]. Developmental & Comparative Immunology, 2020, 104:103567.doi:10.1016/j.dci.2019.103567.
[17]	Xu H Z, Liu H X, Liu C R, Shangguan X Y, Cheng X, Zhang R F, Lu Y T, Li P, Cai Y J. Molecular characterization and antibacterial ability of galectin-3 and galectin-9 in Onychostoma macrolepis[J].Developmental & Comparative Immunology, 2022, 128:104333.doi:10.1016/j.dci.2021.104333.
[18]	Tian M Y, Yang N, Zhang L, Fu Q, Tan F H, Li C. Expression profiling and functional characterization of galectin-3 of turbot(Scophthalmus maximus L.) in host mucosal immunity[J].Fish & Shellfish Immunology, 2019, 84:333-340.doi:10.1016/j.fsi.2018.10.009.
[19]	Yang Y, Wu B L, Li W B, Han F. Molecular characterization of galectin-3 in large yellow croaker Larimichthys crocea functioning in antibacterial activity[J]. International Journal of Molecular Sciences, 2023, 24(14):11539.doi:10.3390/ijms241411539.
[20]	Patel D M, Kitani Y, Korsnes K, Iversen M H, Brinchmann M F. A truncated galectin-3 isolated from skin mucus of Atlantic salmon Salmo salar binds to and modulates the proteome of the gram-negative bacteria Moritella viscosa[J].Marine Drugs, 2020, 18(2):102.doi:10.3390/md18020102.
[21]	王文博, 刘品, 窦玲玲, 王平, 宁越, 惠瑞敏, 冯亚龙, 李爱华, 张义兵. 中国大鲵研究概况[J]. 水生生物学报, 2021, 45(2):464-472.doi:10.7541/2021.2020.184.
	Wang W B, Liu P, Dou L L, Wang P, Ning Y, Xi R M, Feng Y L, Li A H, Zhang Y B. A survey of Chinese giant salamander(Andrias davidianus)[J]. Acta Hydrobiologica Sinica, 2021, 45(2):464-472.
[22]	牟洪民, 李媛, 姚俊杰, 马珊. 大鲵生物学研究的新进展[J]. 水产科学, 2011, 30(8):513-516.doi:10.16378/j.cnki.1003-1111.2011.08.018.
	Mu H M, Li Y, Yao J J, Ma S. A review:current research on biology of Chinese giant salamander[J]. Fisheries Science, 2011, 30(8):513-516.
[23]	张树明, 孙增民, 夏广济, 魏美乐. 陕西大鲵资源养护与产业发展[J]. 中国水产, 2010(7):29-30.doi:10.3969/j.issn.1002-6681.2010.07.013.
	Zhang S M, Sun Z M, Xia G J, Wei M L. Conservation of giant salamander(Andrias davidianus)resources and industrial development in Shaanxi[J]. Aquarium, 2010(7):29-30.
[24]	Janda J M, Abbott S L. The Genus Aeromonas:taxonomy,pathogenicity,and infection[J]. Clinical Microbiology Reviews, 2010, 23(1):35-73.doi:10.1128/cmr.00039-09.
[25]	周小愿, 蔺爱英, 边保团, 贾秋红, 张星朗. 中国大鲵疾病研究进展[J]. 山地农业生物学报, 2012, 31(3):255-260.doi:10.15958/j.cnki.sdnyswxb.2012.03.023.
	Zhou X Y, Lin A Y, Bian B T, Jia Q H, Zhang X L. Progress on giant salamander diseases research[J]. Journal of Mountain Agriculture and Biology, 2012, 31(3):255-260.
[26]	钟蕾, 刘大志, 肖调义, 刘晓燕, 陈开健. 大鲵细菌性败血症病原分离及生物学特性研究[J]. 生物学杂志, 2010, 27(4):11-14.doi:10.3969/j.issn.1008-9632.2010.04.011.
	Zhong L, Liu D Z, Xiao T Y, Liu X Y, Chen K J. Aetiological isolation of the bacterial septicemia of giant salamander and its biological characteristics[J]. Journal of Biology, 2010, 27(4):11-14.
[27]	张晗, 邓捷, 赵虎, 孔飞, 马红英, 姜维, 张红星, 王启军. 大鲵细菌性疾病研究进展[J]. 河北渔业, 2020(3):50-56,62.doi:10.3969/j.issn.1004-6755.2020.03.013.
	Zhang H, Deng J, Zhao H, Kong F, Ma H Y, Jiang W, Zhang H X, Wang Q J. Research progress on bacterial disease of Chinese giant salamander[J]. Hebei Fisheries, 2020(3):50-56,62.
[28]	张联丽, 张向前, 白占涛, 王高学. 大鲵赤皮病病原分离鉴定及防治试验[J]. 西北农林科技大学学报(自然科学版), 1999, 27(4):71-74.doi:10.3321/j.issn:1671-9387.1999.04.016.
	Zhang L L, Zhang X Q, Bai Z T, Wang G X. Isolation and identification of the pathogen and control test of the disease in the giant salamander,Andrias davidianus[J].Journal of Northwest Agriculture and Forestry University(Natural Science Edition), 1999, 27(4):71-74.
[29]	王慧, 杨文佳, 骆建林, 李国勇, 张婷, 白禹, 李灿. 大鲵抗菌肽基因KK1/KK5/KK8对嗜水气单胞菌的侵染响应[J]. 南方农业学报, 2018, 49(8):1627-1632.doi:10.3969/j.issn.2095-1191.2018.08.23.
	Wang H, Yang W J, Luo J L, Li G Y, Zhang T, Bai Y, Li C. Response of Andrias davidianus antimicrobial peptide genes to infection of Aeromonas hydrophila[J].Journal of Southern Agriculture, 2018, 49(8):1627-1632.
[30]	Yang H, Lan Q J, Liu R R, Cui D, Liu H X, Xiong D M, Li F G, Liu X L, Wang L X. Characterization of galectin-1 from Chinese giant salamanders Andrias davidianus and its involvements during immune response[J]. Developmental & Comparative Immunology, 2017, 70:59-68.doi:10.1016/j.dci.2017.01.004.
[31]	Davidson P J, Davis M J, Patterson R J, Ripoche M A, Poirier F, Wang J L. Shuttling of galectin-3 between the nucleus and cytoplasm[J]. Glycobiology, 2002, 12(5):329-337.doi:10.1093/glycob/12.5.329.
[32]	Hsu D K, Liu F T. Regulation of cellular homeostasis by galectins[J]. Glycoconjugate Journal, 2002, 19(7-9):507-515.doi:10.1023/B:GLYC.0000014080.95829.52.
[33]	仉晓文. 甲壳类C-型凝集素的功能研究[D]. 济南: 山东大学, 2011.doi:10.7666/d.Y1939356.
	Zhang X W. Functional studies on the C-type lectins of crustaceans[D]. Jinan: Shandong University,2011.
[34]	Chen M K, Liu X, Zhou J, Wang X, Liu R T, Peng H Y, Li B Y, Cai Z L, Jiang C. Molecular characterization and expression analysis of galectins in Japanese pufferfish(Takifugu rubripes)in response to Vibrio harveyi infection[J]. Fish & Shellfish Immunology, 2019, 86:347-354.doi:10.1016/j.fsi.2018.11.054.
[35]	Zhu J J, Wei M, Wang Q H, Ao Q W, Tan Y, Luo Y J, Wang H, Jiang H S, Hu Q M. Characterization and expression of galectin-3 after Streptococcus agalactiae and Aeromonas hydrophila challenge in GIFT strain Nile tilapia(Oreochromis niloticus)[J]. Fish & Shellfish Immunology, 2019, 86:974-980.doi:10.1016/j.fsi.2018.12.036.
[1]	吕常欢. 大黄鱼凝集素galectin-9和Nattectin-like的功能研究[D]. 厦门: 集美大学, 2016.doi:10.7666/d.D845490.
	Lyu C H. Functional study on galectin-9 and nattectin-like from Pseudosciaena crocea[D]. Xiamen: Jimei University,2016.
[2]	刘品, 王文博. 大鲵蛙病毒致病分子机制及免疫防控策略研究进展[J]. 水产科学, 2022, 41(6):1072-1082.doi:10.16378/j.cnki.1003-1111.21060.
	Liu P, Wang W B. Progress on pathogenesis molecular mechanism and immune control strategies of Chinese giant salamander Andrias davidianus ranavirus:a review[J]. Fisheries Science, 2022, 41(6):1072-1082.
[3]	Almkvist J, Karlsson A. Galectins as inflammatory mediators[J]. Glycoconjugate Journal, 2002, 19(7):575-581.doi:10.1023/B:GLYC.0000014088.21242.e0.
[4]	Leffler H, Carlsson S, Hedlund M, Qian Y N, Poirier F. Introduction to galectins[J]. Glycoconjugate Journal, 2002, 19(7):433-440.doi:10.1023/B:GLYC.0000014072.34840.04.
[5]	Nio-Kobayashi J. Tissue and cell-specific localization of galectins,β-galactose-binding animal lectins,and their potential functions in health and disease[J]. Anatomical Science International, 2017, 92(1):25-36.doi:10.1007/s12565-016-0366-6.
[6]	Wang D W, You D, Li L. Galectin-3 regulates chemotherapy sensitivity in epithelial ovarian carcinoma via regulating mitochondrial function[J]. The Journal of Toxicological Sciences, 2019, 44(1):47-56.doi:10.2131/jts.44.47.
[7]	Darrow A L, Shohet R V. Galectin-3 deficiency exacerbates hyperglycemia and the endothelial response to diabetes[J]. Cardiovascular Diabetology, 2015, 14(1):73.doi:10.1186/s12933-015-0230-3.
[8]	Gruszewska E, Cylwik B, Gińdzieńska-Sies'kiewicz E, Kowal-Bielecka O, Mroczko B, Chrostek L. Diagnostic power of galectin-3 in rheumatic diseases[J]. Journal of Clinical Medicine, 2020, 9(10):3312.doi:10.3390/jcm9103312.

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中国大鲵Galectin-3基因克隆、生物信息学及表达特性分析

Cloning,Bioinformatics and Expression Characteristics Analysis of Galectin-3 Gene in Chinese Giant Salamander

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