姜黄素通过促进自噬改善TGF-&#x003B2;1诱导的BEND上皮-间充质转化

doi:10.7668/hbnxb.20195408

摘要/Abstract

摘要：

为研究姜黄素(CUR)通过调控自噬改善转化生长因子-β1(TGF-β1)诱导的奶牛子宫内膜上皮细胞系(BEND)上皮-间充质转化(EMT)的作用机制,使用不同浓度TGF-β1(2.5,5.0,10.0 ng/mL)诱导EMT模型;应用Western Blot检测不同浓度姜黄素(5,10,20 μmol/L)对TGF-β1诱导的BEND EMT相关蛋白和自噬相关蛋白表达的影响;免疫荧光检测细胞EMT相关蛋白(E-cadherin、N-cadherin、α-SMA)的表达水平;使用自噬抑制剂氯喹(CQ)(10 μmol/L)处理BEND,检测EMT相关蛋白和自噬相关蛋白的表达。TGF-β1诱导的BEND中E-cadherin、Beclin-1和LC3蛋白表达水平显著降低,N-cadherin、α-SMA和P62蛋白表达水平显著上升;而姜黄素处理能够显著增加E-cadherin、Beclin-1、LC3蛋白表达水平,显著降低N-cadherin、α-SMA和P62蛋白表达水平;CQ处理后相较于姜黄素处理能够显著增加N-cadherin、α-SMA、P62和LC3蛋白表达水平,显著降低E-cadherin和Beclin-1蛋白表达水平。姜黄素可以通过促进自噬改善TGF-β1诱导的BEND EMT功能。

关键词: 姜黄素, 转化生长因子β1, 奶牛子宫内膜上皮细胞, 自噬, 上皮-间充质转化

Abstract:

To study the mechanism of curcumin(CUR)improving epithelial-mesenchymal transition(EMT)of bovine endometrial epithelial cells(BEND)induced by transforming growth factor-β1(TGF-β1)by regulating autophagy,different concentrations of TGF-β1(2.5,5.0 and 10.0 ng/mL)were used to induce the EMT model.Western Blot was used to detect the effects of different concentrations of curcumin(5,10 and 20 μmol/L)on the expression of the EMT-related proteins and autophagy-related proteins in BEND induced by TGF-β1.The expression levels of the EMT-related proteins(E-cadherin,N-cadherin and α-SMA)were detected by immunofluorescence.BEND was treated with autophagy inhibitor chloroquine(CQ)(10 μmol/L)to detect the expression of the EMT-related proteins and autophagy-related proteins.The expression levels of E-cadherin,Beclin-1 and LC3 proteins in BEND induced by TGF-β1 were significantly decreased,and the expression levels of N-cadherin,α-SMA and P62 proteins were significantly increased.Curcumin treatment could significantly increase the expression levels of E-cadherin,Beclin-1 and LC3 proteins,and significantly reduce the expression levels of N-cadherin,α-SMA and P62 proteins.Compared with curcumin treatment,CQ treatment significantly increased the expression levels of N-cadherin,α-SMA,P62 and LC3 proteins,and significantly decreased the expression levels of E-cadherin and Beclin-1 proteins.Curcumin can improve TGF-β1 induced BEND EMT function by promoting autophagy.

Key words: Curcumin, TGF-β1, Bovine endometrial epithelial cells, Autophagy, Epithelial-mesenchymal transition

中图分类号:

R285.5

王林楠, 李相辰, 王冠清, 屠久速, 杨玉秋, 宋亮丽. 姜黄素通过促进自噬改善TGF-β1诱导的BEND上皮-间充质转化[J]. 华北农学报, 2025, 40(5): 224-230. doi: 10.7668/hbnxb.20195408.

WANG Linnan, LI Xiangchen, WANG Guanqing, TU Jiusu, YANG Yuqiu, SONG Liangli. Curcumin Improves TGF-β1-induced BEND Epithelial-Mesenchymal Transition by Promoting Autophagy[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 224-230. doi: 10.7668/hbnxb.20195408.

http://www.hbnxb.net/CN/Y2025/V40/I5/224

图/表 4

图1 TGF-β1对BEND中EMT和自噬相关蛋白表达的影响 A—B.EMT相关蛋白表达;C—D.自噬相关蛋白表达。不同小写字母表示差异显著(P<0.05)。图2,4同。

Fig.1 The effects of TGF-β1 on the expression of EMT and autophagy-related proteins in BEND A—B.EMT-related proteins expression;C—D.Autophagy-related proteins expression. Different lowercase letters showed significant difference(P<0.05).The same as Fig.2,4.

图2 姜黄素(CUR)对TGF-β1诱导的BEND中EMT和自噬相关蛋白表达的影响 A—B.EMT相关蛋白表达;C—D.自噬相关蛋白表达。

Fig.2 The effects of curcumin(CUR)on the expression of EMT and autophagy-related proteins in BEND induced by TGF-β1 A—B.EMT-related proteins expression; C—D.Autophagy-related proteins expression.

图3 免疫荧光法检测姜黄素对TGF-β1诱导的BEND EMT相关蛋白表达的影响 EMT相关蛋白免疫荧光(400×)。

Fig.3 The effect of curcumin on the expression of BEND EMT-related proteins induced by TGF-β1 was detected by immunofluorescence Immunofluorescence expression of EMT-related proteins (400×).

图4 姜黄素对TGF-β1诱导的BEND EMT的作用机制

Fig.4 The mechanism of curcumin on TGF-β1-induced BEND EMT

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姜黄素通过促进自噬改善TGF-β1诱导的BEND上皮-间充质转化

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姜黄素通过促进自噬改善TGF-β1诱导的BEND上皮-间充质转化

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