蓖麻RcHSF基因家族鉴定与冷胁迫下的表达模式分析

doi:10.7668/hbnxb.20191312

华北农学报 ›› 2020, Vol. 35 ›› Issue (5): 62-71. doi: 10.7668/hbnxb.20191312

所属专题： 生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

蓖麻RcHSF基因家族鉴定与冷胁迫下的表达模式分析

刘栩铭^1,2, 李敏³, 段琼^1,2, 张洪雨^1,2, 孟迪^1,2, 张丽雪^1,2, 张继星^1,2,4, 王晓宇^1,2,4

1. 内蒙古民族大学生命科学与食品学院, 内蒙古通辽 028000;
2. 内蒙古民族大学科尔沁植物逆境生物学研究所, 内蒙古通辽 028000;
3. 内蒙古民族大学农学院, 内蒙古通辽 028000;
4. 内蒙古自治区高校蓖麻产业工程技术研究中心, 内蒙古自治区蓖麻育种重点实验室, 内蒙古自治区蓖麻产业协同创新培育中心, 内蒙古通辽 028000

收稿日期:2020-06-11 出版日期:2020-10-28
通讯作者: 王晓宇(1987-),男,内蒙古通辽人,副教授,博士,主要从事植物抗逆分子生物学研究;张继星(1971-),男,内蒙古呼和浩特人,教授,博士,主要从事植物抗逆分子生物学研究。
作者简介:刘栩铭(1994-),男,内蒙古阿拉善人,硕士,主要从事植物抗逆分子生物学研究。
基金资助:
国家自然科学基金项目（31701467；31760399）；内蒙古自治区高等学校“青年科技英才”支持计划项目（NJYT-20-B25）；国家服务特殊需求蒙药学博士点建设专项开放基金项目（MDMYBJ2019008）；内蒙古民族大学博士科研启动基金项目（BS398）；内蒙古民族大学自治区科技储备项目（2018NDCB05-3）

Identification of RcHSF Gene Family in Castor and Analysis of Expression Pattern under Cold Stress

LIU Xuming^1,2, LI Min³, DUAN Qiong^1,2, ZHANG Hongyu^1,2, MENG Di^1,2, ZHANG Lixue^1,2, ZHANG Jixing^1,2,4, WANG Xiaoyu^1,2,4

1. College of Life Sciences and Food Engineering, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Horqin Plant Stress Biology Research Institute of Inner Mongolia University for Nationalities, Tongliao 028000, China;
3. Agricultural College, Inner Mongolia University for Nationalities, Tongliao 028000, China;
4. Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Inner Mongolia Key Laboratory of Castor Breeding, Inner Mongolia Collaborative Innovation Cultivate Center for Castor, Tongliao 028000, China

Received:2020-06-11 Published:2020-10-28

摘要/Abstract

摘要： 为明确蓖麻HSF基因家族的结构特征与冷胁迫下的表达模式，利用序列比对法实现蓖麻HSF基因家族成员的全基因组鉴定，通过生物信息学手段对基因家族成员的序列结构、理化性质、染色体定位、启动子顺式作用元件、蛋白保守结构域、系统进化关系以及聚类方式进行分析，并结合RNA-Seq与qRT-PCR对RcHSFs的表达水平进行验证。结果表明，蓖麻基因组中含有19个RcHSF基因家族成员；基因分析发现RcHSFs分别含有1~3个外显子，定位在17个染色体片段上且呈不均匀分布，其中大多数RcHSFs均含有AAGAA-motif、MYB与MYC元件，暗示其响应植物激素与逆境胁迫；蛋白分析表明，RcHSFs按照基序类型与排布方式被聚为A、B、C 3类，推测不同类型HSF的蛋白功能存在差异，尽管所有成员均存在不同程度的motif缺失，但结构域高度保守；RNA-Seq分析发现仅RcHSF4与RcHSF10受冷胁迫诱导表达，与qRT-PCR验证结果一致。结果为RcHSFs基因的克隆与功能研究提供重要的理论基础，为蓖麻抗冷新种质资源的建立提供候选基因。

关键词: 蓖麻, HSF基因家族, 冷胁迫, 表达模式

Abstract: The purpose of this study was to illustrate the structure traits of HSF genes family in castor and its expression pattern in different tissues. The bioinformatics method was used to analyze the sequence structure, physical and chemical properties, chromosome positioning, and cis-acting elements of promoters, the conserved domains, phylogenetic relationships, and clustering methods of the encoded protein. RNA-Seq and qRT-PCR was used to assess the expression of RcHSFs. The results showed that the castor genome contained 19 members of the RcHSF gene family.Gene structure analysis showed that RcHSFs contained 1-3 exons, and were located on 17 chromosome fragments with uneven distribution; most RcHSFs contained AAGAA-motif, MYB and MYC elements, which indicated that these genes were responsive to plant hormone and stress; Protein analysis showed that RcHSFs were grouped into A, B, and C categories according to motif type and arrangement, which indicated that the protein functions of different types of HSF are different. Although all members have different degrees of motif deletion, the domain is highly conserved;RNA-Seq analysis showed that only RcHSF4 and RcHSF10 were induced by cold stress, which was consistent with qRT-PCR verification results. This result provides an important theoretical basis for the cloning and functional research of RcHSFs, and also provides candidate genes for the establishment of new germplasm resources of castor cold resistance.

Key words: Castor, HSF gene family, Cold stress, Expression pattern

中图分类号:

Q78
S563.03

刘栩铭, 李敏, 段琼, 张洪雨, 孟迪, 张丽雪, 张继星, 王晓宇. 蓖麻RcHSF基因家族鉴定与冷胁迫下的表达模式分析[J]. 华北农学报, 2020, 35(5): 62-71. doi: 10.7668/hbnxb.20191312.

LIU Xuming, LI Min, DUAN Qiong, ZHANG Hongyu, MENG Di, ZHANG Lixue, ZHANG Jixing, WANG Xiaoyu. Identification of RcHSF Gene Family in Castor and Analysis of Expression Pattern under Cold Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 62-71. doi: 10.7668/hbnxb.20191312.

http://www.hbnxb.net/CN/Y2020/V35/I5/62

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蓖麻RcHSF基因家族鉴定与冷胁迫下的表达模式分析

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蓖麻RcHSF基因家族鉴定与冷胁迫下的表达模式分析

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