大叶落地生根细胞周期蛋白依赖激酶<em>KdCDK</em>基因克隆及表达分析

doi:10.7668/hbnxb.2017.03.006

华北农学报 ›› 2017, Vol. 32 ›› Issue (3): 33-41. doi: 10.7668/hbnxb.2017.03.006

所属专题： 生物技术；

大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析

朱敏群¹, 梁小红², 黄慧青¹, 欧素荣¹, 葛永强¹, 张利娟³, 钟天秀⁴

1. 深圳市日昇园林绿化有限公司, 广东深圳 518040;
2. 北京林业大学林学院草坪研究所, 北京 100083;
3. 暨南大学深圳旅游学院, 广东深圳 518053;
4. 华南农业大学林学与风景园林学院, 广东省草业工程技术研究中心, 广东广州 510642

收稿日期:2017-04-12 出版日期:2017-06-28
作者简介:朱敏群(1978-),女,江西赣州人,工程师,主要从事园林绿化工作。
基金资助:
深圳市科技计划项目（CXZZ20140418110342522）；北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目

cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase (KdCDK) Gene in Kalanchoe daigremontiana

ZHU Minqun¹, LIANG Xiaohong², HUANG Huiqing¹, OU Surong¹, GE Yongqiang¹, ZHANG Lijuan³, ZHONG Tianxiu⁴

1. Shenzhen Risheng Landscape Company Limited, Shenzhen 518040, China;
2. Turfgrass Research Institute, The College of Forestry, Beijing Forestry University, Beijing 100083, China;
3. Shenzhen Tourism College of Ji'nan University, Shenzhen 518053, China;
4. College of Forestry and Landscape Architecture, South China Agricultural University, Guangdong Engineering Research Center of Grassland Science, Guangzhou 510642, China

Received:2017-04-12 Published:2017-06-28

摘要/Abstract

摘要： 为了更好理解大叶落地生根中细胞周期调控的分子机制，利用RACE-PCR技术从大叶落地生根中克隆了1个新的基因KdCDK。该基因编码295个氨基酸残基，分子量为34.12 kDa，等电点为6.15，其开放阅读框长度为888 bp。其蛋白与笋瓜CDK蛋白的同源性最高，属于CDKA类蛋白家族。实时荧光定量PCR分析表明，该蛋白基因在大叶落地生根的根中表达量最高，且受渗透胁迫（甘露醇）的诱导上调表达。从大叶落地生根中克隆出KdCDK基因，为将来该基因的功能研究打下了基础。

关键词: 大叶落地生根, 细胞周期蛋白依赖激酶, 基因表达

Abstract: To better understand the molecular mechanisms of CDKs involved in Kalanchoe daigremontiana,an A-Type CDK gene, KdCD K,was identified using rapid amplification of cDNA end(RACE)PCR. KdCDK gene consists of an ORF of 888 bp that was predicted to encode a 295 amino acid residue-long protein of 34.12 kDa with an isoelectric point of 6.15.KdCDK was related most closely to CmCDK and was clustered to CDKA subfamily.Real-time PCR analysis revealed that KdCDK transcript was expressed highly in root and upregulated under osmotic stress.This study characterized the novel KdCDK gene from Kalanchoe daigremontiana for the first time and the results would be useful for further functional determination of the gene.

Key words: Kalanchoe daigremontiana, Cyclin-dependent kinase, Gene expression

中图分类号:

朱敏群, 梁小红, 黄慧青, 欧素荣, 葛永强, 张利娟, 钟天秀. 大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析[J]. 华北农学报, 2017, 32(3): 33-41. doi: 10.7668/hbnxb.2017.03.006.

ZHU Minqun, LIANG Xiaohong, HUANG Huiqing, OU Surong, GE Yongqiang, ZHANG Lijuan, ZHONG Tianxiu. cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase (KdCDK) Gene in Kalanchoe daigremontiana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 33-41. doi: 10.7668/hbnxb.2017.03.006.

http://www.hbnxb.net/CN/Y2017/V32/I3/33

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大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析

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大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析

cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase (KdCDK) Gene in Kalanchoe daigremontiana

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