花生<em>MAPK</em>基因的筛选和盐胁迫分析

doi:10.7668/hbnxb.2017.03.005

华北农学报 ›› 2017, Vol. 32 ›› Issue (3): 27-32. doi: 10.7668/hbnxb.2017.03.005

所属专题： 油料作物；盐碱胁迫；生物技术；

花生MAPK基因的筛选和盐胁迫分析

孔祥远, 陈冠旭, 秦贵龙, 隋炯明, 乔利仙, 王晶珊, 徐丽丽

青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛 266109

收稿日期:2017-01-25 出版日期:2017-06-28
通讯作者: 徐丽丽(1979-),女,山东诸城人,讲师,硕士,主要从事基因工程研究。
作者简介:孔祥远(1989-),男,山东菏泽人,在读硕士,主要从事作物分子育种研究。
基金资助:
国家自然基金项目（31571705；31301356；31471542）；山东省科技发展计划项目（2014GNC110002）

Screening and Salinity Stress Analysis of MAPK Genes in Peanut

KONG Xiangyuan, CHEN Guanxu, QIN Guilong, SUI Jiongming, QIAO Lixian, WANG Jingshan, XU Lili

College of Life Science, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China

Received:2017-01-25 Published:2017-06-28

摘要/Abstract

摘要： 促有丝分裂原活化蛋白激酶（MAPK）在植物生长发育及抗逆胁迫过程中发挥重要作用。为了解花生中MAPK基因的情况，利用RNA测序技术对花生耐盐突变体（S2）和对照（S4）进行了转录组分析，筛选出了14个MAPK基因，位于花生野生种基因组A组的6条染色体上。聚类分析表明，花生14个MAPK基因中13个可以聚到已报道的4个亚类中。利用花生S2和S4构建了盐胁迫处理前后的表达谱，根据调整后的P值，筛选出6个MAPK基因在S2和（或）S4受盐胁迫诱导表达，分别属于A（1个）和D亚类（5个）。为花生MAPK基因的功能验证和利用奠定了基础。

关键词: 花生, MAPK基因, 盐胁迫, RNA测序

Abstract: Mitogen activated protein kinase (MAPK) plays an important role in plant growth and development,and stress resistance in plant.In order to improve our understanding of the MAPK genes in peanut,RNA-seq was used to analyze one transcriptomic library constructed using a mutant with higher salinity resistance (S2) and its control (S4) in this study,14 MAPK genes were screened and located on six chromosomes of A genome from wild species.Clustering analysis showed that 13 out of 14 MAPK genes were assorted to four subgroups reported previously.Digital gene expression profiles were constructed using S2 and S4 samples before and after salinity treatment,according to the adjusted P value,six MAPK genes were found to be responsive to salinity treatment in S2 and (or) S4,which belonged to A (1)and D (5) subgroups,respectively.This study can provide the basis for functional identification and application of MAPK genes in peanut.

Key words: Peanut, MAPK gene, Salinity stress, RNA-seq

中图分类号:

Q78
S565.03

孔祥远, 陈冠旭, 秦贵龙, 隋炯明, 乔利仙, 王晶珊, 徐丽丽. 花生MAPK基因的筛选和盐胁迫分析[J]. 华北农学报, 2017, 32(3): 27-32. doi: 10.7668/hbnxb.2017.03.005.

KONG Xiangyuan, CHEN Guanxu, QIN Guilong, SUI Jiongming, QIAO Lixian, WANG Jingshan, XU Lili. Screening and Salinity Stress Analysis of MAPK Genes in Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 27-32. doi: 10.7668/hbnxb.2017.03.005.

http://www.hbnxb.net/CN/Y2017/V32/I3/27

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Screening and Salinity Stress Analysis of MAPK Genes in Peanut

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