摘要： 为筛选重要地方鸭品种——高邮鸭早期肌肉生长标记，利用连接酶检测反应，以高邮鸭群体为研究对象，筛选肌分化因子基因MyoD1第一内含子区域的SNP位点，以期获得高邮鸭早期生长分子标记。筛选获得MyoD1基因第一内含子区的位点突变T→A，检测到AA，TA和TT 3种基因型，其中AA和TA是优势基因型，处于Hardy-Weinberg平衡状态。关联分析结果表明TA基因型群体在49日龄的体重、胸肌重、胸肌横截面积、胸肌纤维直径以及胸肌纤维密度均显著高于AA、TT基因型群体（P<0.05），TT和AA基因型间差异不显著（P>0.05）。相较于传统育种，分子标记辅助育种具有快速、精准的优点。结果预示TA基因型可作为高邮鸭胸肌早期选择的分子标记。

关键词: 高邮鸭, MyoD1基因, 多态性, 胸肌

Abstract: In order to explore the molecular marker for breeding the Gaoyou duck breed. In this study,based on the LDR method,a new SNPs was found on Gaoyou duck MyoD1 gene with its transition T→A. Three genotypes AA,TA and TT were found in the population,and AA and TA were dominant genotypes. The transition of this locus was at Hardy-Weinberg equilibrium in Gaoyou duck. Relationship with the breast muscle development traits was analysised. Statistical analysis revealed that a higher value of body weight,breast weight,breast fiber area,breast average diameter and breast density for individuals with genotype TA than AA and TT(P<0.05),and there was no difference between the genotype AA and TT(P>0.05). These results indicated that genotype TA could be used as molecular marker for developing of Gaoyou duck breast muscle,further studies on a bigger population size and more duck breeds are needed to confirm the observed effect of MyoD1 genotypes on breast muscle traits.

Key words: Gaoyou duck, MyoD1 gene, Polymorphism, Breast muscle

中图分类号: 

• Q78

• S834.03